Quantifying ecological effects of sedimentation in streams in differing land use management zones

<p>This ecological and geomorphological assessment of Horokiri Stream and Ration Creek was conducted across four longitudinal zones to explore the effects of sediment delivery, run-off, channel form, riparian and in-stream habitat. The Horokiri Stream channel has moved approximately 7 metres westward over the last 20 years, with both banks now covered in long grass, flaxes, natives with a mix of tall canopy trees. Looking at stream, Spearman’s for Ration at Figure 27 (n = 16, rho -0.243, p = 0.36) as deposited sediment increased, MCI decreased, non-significant. Spearman’s for Horokiri at Figure 28 (n = 16, rho 0.247, p = 0.35) as MCI increased with sediment, non-significant. Results from upstream of the riparian zones showed more deposited fine sediment. However, within both the riparian zones the sediment deposition was much lower. The native riparian planting along the stream banks had a positive effect on reducing sedimentation. The findings support the concept that the restoration of riparian zones with buffer widths exceeding 10 metres can improve stream habitat and invertebrate health. There was no relationship between flow and deposition rate P(X2>241.84) = 0.24. Figure 24 shows deposited sediment on MCI depending on land use groups (X2 = 11.81, df = 4, p = 0.019). No statistically significant differences were found (comparing the effect of sediment between different land use management groups). An experiment investigated a disturbance hypothesis in both Ration Creek and Horokiri Stream was conducted during February 2019. The experiment was designed to be long enough to study the effects of four weekly pulse flushing events created by scrapping the stream bed with a drain drag tool and the effects of a press sustained disturbance on the macroinvertebrate community. I measured the sediment and the macroinvertebrate captured in each trap within the experiment site every seven days. My prediction was that macroinvertebrate communities subject to sustained fine sediment delivery (press disturbance) are affected by simulated pulse flushing events (pulse disturbance). A comparison of sediment depositional rate before and after the manipulative experiment (Figure 36) showed higher sediment deposition after the pulse flushing events (1.55 W/A/D) compared to before during the assessment phase (0.88 W/A/D) in Horokiri (t = 2.35, df = 8.95, p = 0.04), but no significant difference before (1.57 W/A/D) or after (1.38 W/A/D) in Ration (t = -0.818, df = 7.71, p = 0.44). It appeared that the smaller riparian buffer width of 2-5m at Ration Creek did not limit sediment deposition. The effects of sediment disturbance in the experiment reflect the rapid ability of macroinvertebrates to respond to sediment by drifting out of unsuitable areas. The weekly pulse disturbance events resulted in increased sediment deposition compared to the background levels of sediment deposition (indicative of a press disturbance) in both streams. As pulse disturbance events increased, the number of macroinvertebrate taxa decreased. Horokiri Stream invertebrate communities declined by 33% compared to Ration Creek which declined by 50%.</p>

Quantifying ecological effects of sedimentation in streams in differing land use management zones

<p>This ecological and geomorphological assessment of Horokiri Stream and Ration Creek was conducted across four longitudinal zones to explore the effects of sediment delivery, run-off, channel form, riparian and in-stream habitat. The Horokiri Stream channel has moved approximately 7 metres westward over the last 20 years, with both banks now covered in long grass, flaxes, natives with a mix of tall canopy trees. Looking at stream, Spearman’s for Ration at Figure 27 (n = 16, rho -0.243, p = 0.36) as deposited sediment increased, MCI decreased, non-significant. Spearman’s for Horokiri at Figure 28 (n = 16, rho 0.247, p = 0.35) as MCI increased with sediment, non-significant. Results from upstream of the riparian zones showed more deposited fine sediment. However, within both the riparian zones the sediment deposition was much lower. The native riparian planting along the stream banks had a positive effect on reducing sedimentation. The findings support the concept that the restoration of riparian zones with buffer widths exceeding 10 metres can improve stream habitat and invertebrate health. There was no relationship between flow and deposition rate P(X2>241.84) = 0.24. Figure 24 shows deposited sediment on MCI depending on land use groups (X2 = 11.81, df = 4, p = 0.019). No statistically significant differences were found (comparing the effect of sediment between different land use management groups). An experiment investigated a disturbance hypothesis in both Ration Creek and Horokiri Stream was conducted during February 2019. The experiment was designed to be long enough to study the effects of four weekly pulse flushing events created by scrapping the stream bed with a drain drag tool and the effects of a press sustained disturbance on the macroinvertebrate community. I measured the sediment and the macroinvertebrate captured in each trap within the experiment site every seven days. My prediction was that macroinvertebrate communities subject to sustained fine sediment delivery (press disturbance) are affected by simulated pulse flushing events (pulse disturbance). A comparison of sediment depositional rate before and after the manipulative experiment (Figure 36) showed higher sediment deposition after the pulse flushing events (1.55 W/A/D) compared to before during the assessment phase (0.88 W/A/D) in Horokiri (t = 2.35, df = 8.95, p = 0.04), but no significant difference before (1.57 W/A/D) or after (1.38 W/A/D) in Ration (t = -0.818, df = 7.71, p = 0.44). It appeared that the smaller riparian buffer width of 2-5m at Ration Creek did not limit sediment deposition. The effects of sediment disturbance in the experiment reflect the rapid ability of macroinvertebrates to respond to sediment by drifting out of unsuitable areas. The weekly pulse disturbance events resulted in increased sediment deposition compared to the background levels of sediment deposition (indicative of a press disturbance) in both streams. As pulse disturbance events increased, the number of macroinvertebrate taxa decreased. Horokiri Stream invertebrate communities declined by 33% compared to Ration Creek which declined by 50%.</p>

Does extracellular DNA mask microbial responses to a pulse disturbance?

A major goal in microbial ecology is to predict how microbial communities will respond to global change. However, DNA-based sequencing that is intended to characterize live microbial communities includes extracellular DNA (exDNA) from non-viable cells. This could obscure relevant microbial responses, particularly to pulse disturbances which kill bacteria and have disproportionate effects on ecosystems. Here, we characterize bacterial communities before and after a drying-rewetting pulse disturbance, using an improved method for exDNA exclusion. We find that exDNA removal is important for detecting subtle yet significant changes in microbial abundance, diversity, and community composition across the disturbance. However, inclusion of exDNA did not obscure results to a large extent, only sometimes altering statistical significance but rarely changing the direction of the response or general conclusions about bacterial disturbance dynamics. Although there may be instances where exDNA removal is essential for accurate representation of microbial communities, our study suggests these scenarios will be difficult to predict a priori. Overall, we found no evidence that certain time points across the distrubance were more affected by exDNA inclusion, nor did the size or composition of exDNA pools accurately predict when exDNA would alter significance levels. However, exDNA dynamics did vary strongly across the two soil types tested.

Differential response of forest-forming seaweeds to elevated turbidity may facilitate ecosystem shifts on temperate reefs

Underwater light is essential for fuelling coastal productivity. However, elevated turbidity, resulting from land-based activities and climate change, is often overlooked as a threat to coastal ecosystems. Understanding how low light, and specifically the temporal delivery of light, affects the productivity of forest-forming species is necessary to predict how ecosystems and species will respond to future increases in turbidity. Outdoor mesocosm experiments were used to compare the low-light tolerance of 2 forest-forming macrophytes that vary in their distribution in relation to turbidity, and investigate how the temporal delivery of light, i.e. press vs. pulse low-light disturbance, affects net primary productivity (NPP). We showed that the kelp Ecklonia radiata, which dominates reefs with low turbidity, is more productive per unit biomass under high-light conditions than the fucoid Carpophyllum flexuosum, which typifies more turbid waters. Under low light, E. radiata suffered greater tissue loss and had lower NPP than C. flexuosum. Under both press and pulse treatments, E. radiata showed significant losses of lamina biomass and reduced NPP, while C. flexuosum showed net growth under press disturbance, and only lost tissue and had reduced NPP under pulse disturbance. The greater tolerance of C. flexuosum to decreased light, and differential responses of E. radiata and C. flexuosum to press and pulse low-light conditions, provide mechanistic support for C. flexuosum being better suited to turbid low-light environments than E. radiata. These results suggest future increases in turbidity may facilitate a shift from kelp-dominated forests to alternate states, resulting in reduced primary productivity.