Nanodispersions of Polyelectrolytes Based on Humic Substances: Isolation, Physico-Chemical Characterization and Evaluation of Biological Activity

Natural polyelectrolytes, including in the form of complexes with colloidal particles, are increasingly used in pharmacy due to the possibility of regulated attachment of medicinal substances and their targeted delivery to the target organ. However, the formation, stability, and molecular-mass characteristics of polyelectrolyte nanodispersions (ND) vary depending on the nature and composition of the medium of their origin. This is due to the lack of standardized approaches to quality control and regulatory documentation for most natural ND. In this paper, we first introduced the isolation, followed by investigations into their physico-chemical properties and bioactivity. Using the dried droplet method, we were able to detect the “coffee ring effect”. Fractographic studies of the surface structure of EHA and FA dried samples using SEM showed its heterogeneity and the presence of submicron particles encapsulated in the internal molecular cavities of polyelectrolyte. FTIR spectroscopy revealed the ND chemical structure of benzo-α-pyron and benzo-γ-pyron, consisting of nanoparticles and a branched frame part. The main elements detected by X-ray fluorescence in humic substance extract and fulvic acid include Si, P, S, K, Ca, Mn, Fe, Cu, Zn, whereas Fe is in high concentrations. The UV-spectra and fluorescent radiation demonstrated the possibility of studying the effect of the fulvate chromone structure on its optical properties. It is shown that dilution of the initial solutions of polyelectrolytes 1:10 contributes to the detection of smaller nanoparticles and an increase in the absolute value of the negative ζ-potential as a factor of ND stability. A study of the EHS effect on the SARS-CoV-2 virus infectious titer in the Vero E6 cell showed the effective against virus both in the virucidal scheme (the SI is 11.90–22.43) and treatment/prevention scheme (the SI is 34.85–57.33). We assume that polyelectrolyte ND prevent the binding of the coronavirus spike glycoprotein to the receptor. Taking into account the results obtained, we expect that the developed approach can become unified for the standardization of the ND natural polyelectrolytes complex, which has great prospects for use in pharmacy and medicine as a drug with antiviral activity.

Driven Qubit by Train of Gaussian-Pulses

The simplest non-dissipative 2-level atom system, a qubit, excited by a train of resonant n-Gaussian laser pulses is investigated. This concerns examination of the averaged atomic variables, the intensity-intensity correlation function, and the transient fluorescent radiation. Analytical formulas for the above expressions are obtained. Computational results show that the transient spectra with the initial ground and coherent atomic states exhibit asymmetric Mollow structure with dip structure, dense oscillation, and narrowing, and depends on the pulse number (n), the repetition time (τR), and the observed time.

Scattered and Fluorescent Photon Track Reconstruction in a Biological Tissue

Appropriate analysis of biological tissue deep regions is important for tumor targeting. This paper is concentrated on photons’ paths analysis in such biotissue as brain, because optical probing depth of fluorescent and excitation radiation differs. A method for photon track reconstruction was developed. Images were captured focusing on the transparent wall close and parallel to the source fibres, placed in brain tissue phantoms. The images were processed to reconstruct the photons most probable paths between two fibres. Results were compared with Monte Carlo simulations and diffusion approximation of the radiative transfer equation. It was shown that the excitation radiation optical probing depth is twice more than for the fluorescent photons. The way of fluorescent radiation spreading was discussed. Because of fluorescent and excitation radiation spreads in different ways, and the effective anisotropy factor,geff, was proposed for fluorescent radiation. For the brain tissue phantoms it were found to be0.62±0.05and0.66±0.05for the irradiation wavelengths 532 nm and 632.8 nm, respectively. These calculations give more accurate information about the tumor location in biotissue. Reconstruction of photon paths allows fluorescent and excitation probing depths determination. Thegeffcan be used as simplified parameter for calculations of fluorescence probing depth.

SPECTRUM OF SPIN-½ SYSTEM DRIVEN BY RESONANT EXPONENTIAL PULSE

The exact operator solution for a system of a single 2-level atom (spin-1/2 system) driven by a short resonant exponential pulse is used to examine the atomic dynamics and to calculate the time-dependent spectrum of the fluorescent radiation. The spectrum is expressed analytically in terms of the incomplete Gamma funtion (of complex arguments). Computational display of the results are presented for various system parameters.