Experimental calibration of trapping methods for addressing bias in arthropod biodiversity monitoring

1.SummaryAll approaches to biodiversity monitoring have inherent biases in the taxa captured, yet, as environments, sampling goals, and conventions vary, it is not uncommon for sampling approaches to be customized to reflect the study objectives, optimizing findings to be locally relevant but at the cost of transferability. Here, we developed a calibration study to directly examine how researcher trap choice affects observations made in insect biodiversity sampling. Sampling efficiency of four types of traps: pitfalls, yellow ramp traps, a novel jar ramp trap, and yellow sticky cards, were compared with respect to an array of biodiversity metrics associated with the arthropods they captured. We found that trapping efficiency and functional groups of arthropods (flying versus ground-crawling) varied by trap type. Pitfalls and jar ramp traps performed similarly for all biodiversity metrics measured, suggesting that jar ramp traps provide a more comparable measurement of ground-crawling arthropod communities to pitfall sampling than the yellow ramp traps and should be considered when pitfall sampling cannot be used. This study illustrates the implications for biodiversity sampling of arthropods in environments with physical constraints on trapping, and the importance of directly comparing adapted methods to established sampling protocol. Future biodiversity monitoring schemes should conduct calibration experiments to provide important information on performance and potential limitations of sampling methodology.

Dietary Animal to Plant Protein Ratio Is Associated with Risk Factors of Metabolic Syndrome in Participants of the AHS-2 Calibration Study

Background: Few research studies have focused on the effects of dietary protein on metabolic syndrome and its components. Our objective was to determine the relationship between the type of dietary protein intake and animal to plant (AP) protein ratio with metabolic syndrome and its components. Methods: This population-based study had a cross sectional design and conducted on 518 participants of the Adventist Health Study 2 (AHS-2) Calibration Study. Two sets of three dietary 24-h recalls were obtained six months apart. Anthropometric measures and biochemical tests were performed in clinics. Regression calibration models were used to determine the association of type of dietary protein with metabolic syndrome and its components (raised triglyceride, raised blood pressure, reduced high-density lipoprotein cholesterol (HDL), raised fasting blood glucose and increased waist circumference). Results: The likelihood of metabolic syndrome was lower in those with higher total dietary protein and animal protein intake (p = 0.02).Total protein (β = 0.004, [95%CI: 0.002, 0.007]), animal protein intake (β = 0.004, [95%CI: 0.001, 0.007]) and AP protein intake ratio (β = 0.034, [95%CI: 0.021, 0.047]) were positively associated with waist circumference. Higher AP protein ratio was related to higher fasting blood glucose (β = 0.023, [95%CI: 0.005, 0.041]). Conclusion: Our study suggests that considering a significant amount of plant protein as a part of total dietary protein has beneficial effects on cardiometabolic risk factors.

Evaluation of otolith microstructures for ageing of larval white sturgeon

Sturgeon populations are endangered worldwide, mainly due to habitat degradation and overexploitation causing recruitment failure. Understanding of early development, survival and growth in sturgeon is limited by a lack of a validated method to directly estimate larval age. In a laboratory calibration study, we reared white sturgeon larvae from hatch for 3 weeks at 12 and 16°C and two feeding regimes (fed and unfed) to determine the usefulness of their vateritic micro-otoconia for larval ageing and the influence of environmental factors on ring structure and size. By marking the otoliths twice at known ages with Alizarin Red S, we were able to confirmed the presence of daily rings in the largest micro-otoconia and the feasibility of ageing larval sturgeon using otoliths. Three observers blind to age and treatment assessed larval age from daily rings with an overall precision of 67-82% and 30-70% accuracy, dependent on larval age. Neither temperature nor feeding had a significant effect on ring width or readability. Thus, ageing with otoliths in sturgeon is a promising tool in sturgeon conservation efforts.

Soil test crop response based phosphorus calibration study for bread wheat in Kofele District, West Arsi Zone Oromia, Ethiopia

Nowadays, a balanced fertilizer recommendation is of paramount importance in order to confirm the security and sustainably increase crop productivity for farmers and other stakeholders. Soil test crop response based phosphorus calibration study in two years (2017 and 2018) was done for bread wheat in kofele district with objectives to assess and evaluate yield response of bread wheat to phosphorus-fertilizer applications in soils that have initial high/medium/low levels of phosphorus on Eutric Vertisols. A composite soil samples collection were made in zigzag method from farmer’s land and analyzed for available P in order to identify the level of the required parameters in the soil to select farmland for actual experiment. Accordingly, phosphorus calibration study treatments include application of 0, 10,20,30,40 and 50 kg P ha-1 with recommended nitrogen 69 kg N ha-1 with RCBD design was used with two replications. The plot size of 5mx4m with a seed rate of 150 kg ha-1 and Ogolcho variety which had been recommended for the area was used. So that the result showed that phosphorus fertilizer application significantly affects yield and yield components of bread wheat. Similarly, phosphorous fertilizer application at different rates increased grain yield of bread wheat by 28 to 44% compared to the control. Furthermore, the study was revealed that phosphorus critical (Pc) point for bread wheat was 19, and phosphorus requirement factor was also 3.30. Therefore, future research should focus on verification of the result on farmland before disseminating the technology to the end-user.

δ2Hn-alkane and δ18Osugar biomarker proxies from leaves and topsoils of the Bale Mountains, Ethiopia, and implications for paleoclimate reconstructions

The hydrogen isotopic composition of leaf wax–derived n-alkane (δ2Hn-alkane) and oxygen isotopic composition of hemicellulose–derived sugar (δ18Osugar) biomarkers are valuable proxies for paleoclimate reconstructions. Here, we present a calibration study along the Bale Mountains in Ethiopia to evaluate how accurately and precisely the isotopic composition of precipitation is imprinted in these biomarkers. n-Alkanes and sugars were extracted from the leaf and topsoil samples and compound–specific δ2Hn-alkane and δ18Osugar values were measured using a gas chromatograph–thermal conversion–isotope ratio mass spectrometer (GC–TC–IRMS). The weighted mean δ2Hn-alkane and δ18Osugar values range from − 186 to − 89‰ and from + 27 to + 46‰, respectively. Degradation and root inputs did not appear to alter the isotopic composition of the biomarkers in the soil samples analyzed. Yet, the δ2Hn-alkane values show a statistically significant species dependence and δ18Osugar yielded the same species–dependent trends. The reconstructed leaf water of Erica arborea and Erica trimera is 2H– and 18O–enriched by + 55 ± 5 and + 9 ± 1‰, respectively, compared to precipitation. By contrast, Festuca abyssinica reveals the most negative δ2Hn-alkane and least positive δ18Osugar values. This can be attributed to “signal–dampening” caused by basal grass leaf growth. The intermediate values for Alchemilla haumannii and Helichrysum splendidum can be likely explained with plant physiological differences or microclimatic conditions affecting relative humidity (RH) and thus RH–dependent leaf water isotope enrichment. While the actual RH values range from 69 to 82% (x̄ = 80 ± 3.4%), the reconstructed RH values based on a recently suggested coupled δ2Hn-alkane –δ18Osugar (paleo–) hygrometer approach yielded a mean of 78 ± 21%. Our findings corroborate (i) that vegetation changes, particularly in terms of grass versus non–grassy vegetation, need to be considered in paleoclimate studies based on δ2Hn-alkane and δ18Osugar records and (ii) that the coupled δ2Hn-alkane –δ18Osugar (paleo–) hygrometer approach holds great potential for deriving additional paleoclimatic information compared to single isotope approaches.