Implantation of toric intraocular lenses with modified marking of the cylindrical component

Purpose. Is to propose a marking of a toric intraocular lens (TIOL) to accurately determine its position in the capsular bag during surgery in patients with corneal astigmatism in a narrow rigid pupil. Material and methods. The study included 19 patients (24 eyes) with corneal astigmatism from 1.25 to 5.0 diopters. The maximum observation period for patients after surgery is 6 months. Results. The mean uncorrected visual acuity (UVA) after surgery was 0.6±0.21. The spherical postoperative component of refraction was 0.09±0.81, the cylindrical component was 0.25±0.52 diopters. During the entire observation period after surgery, stable refraction and visual functions were noted. Conclusion. The proposed improved lens marking technique makes it possible to accurately determine the position of the cylindrical component of the THIOL, ensuring the correct position of the lens under conditions of a narrow rigid pupil. Key words: cataract phacoemulsification, astigmatism, toric intraocular lenses, marking.

Initial experience of virtual-assisted lung mapping utilizing both indocyanine green and indigo carmine

Virtual-assisted lung mapping is a bronchoscopic multiple dye marking technique that facilitates sublobar lung resections for unidentifiable pulmonary tumors. Marking failure reportedly occurs in 10% of cases. To overcome this limitation, we developed indocyanine green virtual-assisted lung mapping that uses indocyanine green in addition to indigo carmine. Here, we report our initial experience of indocyanine green virtual-assisted lung mapping.

Simultaneous Mastopexy Explantation With a Vertical Bipedicle and Novel Open Pattern Marking Technique

There are limited studies in the plastic surgery literature that detail technical considerations in simultaneous breast implant removal and mastopexy procedures. These procedures are difficult, with significant potential for complications and poor cosmesis. The current plastic surgery literature describes a limited number of techniques that address these concerns, virtually all of which are variants of superior or superior medial pedicle vertical pattern mastopexy. This paper details a safe technique for simultaneous explantation and mastopexy with a novel open marking pattern and vertical bipedicle, which can restore breast cosmesis following implant removal. The study will briefly retrospectively review the results of a consecutive series of 86 patients who underwent this procedure from November 2018 to November 2019, with no incidence of partial or total nipple-areola complex necrosis. Thus, the technique is safe and allows the flexibility for intraoperative adjustments that are necessary for these procedures. A future study will conduct a more in-depth analysis of the results. Level of Evidence: 4

Can post-capture photographic identification as a wildlife marking technique be undermined by observer error? A case study using King Cobras in northeast Thailand

Identifying individuals with natural markings is increasing in popularity to non-invasively support population studies. However, applying natural variation among individuals requires careful evaluation among target species, snakes for example have little validation of such methods. Here we introduce a mark-free identification method for King Cobras (Ophiophagus hannah) from the Sakaerat Biosphere Reserve, in northeast Thailand using both subcaudal scale pholidosis (scale arrangement and number) and unique ventral body markings to distinguish individuals. This project aims to evaluate the impact of observer error on individual identification. Observers of varying expertise, will distinguish between King Cobra individuals using identifying photographs from a previous study. We will ask randomly assigned observers to distinguish individuals via: 1) subcaudal pholidosis, 2) ventral body markings, and 3) combination of both measures. Using Bayesian logistic regression, we will assess the probability observers correctly distinguish individuals. Based on exploratory observations, we hypothesise that there will be a high probability of correct identifications using subcaudal pholidosis and ventral body markings. We aim to stimulate other studies implementing identification techniques for scrutinous assessment of such methods, in order to avoid subsequent errors during long-term population studies.

A novel fluorescent lung-marking technique using the photodynamic diagnosis endoscope system and vitamin B2

OBJECTIVES For small pulmonary nodules that are unidentifiable by palpation or in endoscopic surgeries wherein palpation is not feasible, visualizing their location is necessary when performing pulmonary sublobar resection procedures, such as wedge resection or segmentectomy. We invented a new transbronchial lung-marking technique using the photodynamic diagnosis endoscope system and vitamin B2 and examined its feasibility and safety via porcine studies. METHODS We established the marking procedure in pigs and examined the marking clarity and size, fluorescence intensity and duration and possible complications. In another study, sublobar resection for virtual target lesions was performed in pigs based on the fluorescent markings. The procedure duration, marking visibility, surgical margin from the lesions and technique-related complications were assessed. RESULTS All 36 markings in 6 pigs were identifiable and were widely distributed over the right lung. The median diameter and fluorescence intensity at 60 min after marking were 6.0 (5.5–6.7) mm and 137.5 (122–168), respectively. All 18 markings for the 6 virtual target lesions (3 markings for each target) were clearly identified, and all target lesions were found in the resected specimens. The median duration per marking was 244 (194–255) seconds. The shortest median surgical margin from a target lesion was 11.5 (9.3–13.5) mm. No procedure-related complications were observed. CONCLUSIONS This novel transbronchial fluorescent lung-marking technique was useful and safe in sublobar resections for small non-palpable pulmonary lesions.

Validation of Acrylic Paint as a Marking Technique for Examining Mating Success of the Black Soldier Fly (Diptera: Stratiomyidae)

The black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), is mass produced worldwide for use in waste management and the production of an alternative protein for use as feed. However, few publications have explored its adult behavior, particularly mating, as a means to optimize egg production in colony. In addition, there is limited knowledge of appropriate marking techniques to explore the mating behavior of this insect. The goal of this study was to validate water-based acrylic paint pens as a possible marking tool for behavioral studies with adult black soldier flies. Adult black soldier flies (<24-h old) were marked with green, gold, red, or white acrylic paint. Adult longevity, as well as the number of matings (mating success) were recorded for each treatment. Regardless of marking, 1) adult females lived ~7 d, whereas males lived 9 d, 2) mating frequency peaked 2 d after initiation of the experiment, 3) approximately 30% of adults across treatments were recorded mating, and 4) egg production and hatch rate were not affected. This marking technique did not affect the parameters measured, suggesting that it is suitable for experiments requiring a reliable marking technique.