Investigating the influence of block copolymer micelle length on cellular uptake and penetration in a multicellular tumor spheroid model

The efficient penetration of drug nanocarriers into tumors is an important prerequisite for therapeutic and diagnostic success.

Minimization of ion transport resistance: diblock copolymer micelle derived nitrogen-doped hierarchically porous carbon spheres for superior rate and power Zn-ion capacitors

N-doped hierarchically porous carbon spheres are fabricated for Zn-ion capacitors, and they possess isotropic Zn2+ diffusion routes and abundant active sites, resulting in minimized transport resistance for fast Zn2+ storage and high capacity.

Ultrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood

Development of plasmonic biosensors combining reliability and ease of use is still a challenge. Gold nanoparticle arrays made by block copolymer micelle nanolithography (BCMN) stand out for their scalability, cost-effectiveness and tunable plasmonic properties, making them ideal substrates for fluorescence enhancement. Here, we describe a plasmon-enhanced fluorescence immunosensor for the specific and ultrasensitive detection of Plasmodium falciparum lactate dehydrogenase (PfLDH)—a malaria marker—in whole blood. Analyte recognition is realized by oriented antibodies immobilized in a close-packed configuration via the photochemical immobilization technique (PIT), with a top bioreceptor of nucleic acid aptamers recognizing a different surface of PfLDH in a sandwich conformation. The combination of BCMN and PIT enabled maximum control over the nanoparticle size and lattice constant as well as the distance of the fluorophore from the sensing surface. The device achieved a limit of detection smaller than 1 pg/mL (<30 fM) with very high specificity without any sample pretreatment. This limit of detection is several orders of magnitude lower than that found in malaria rapid diagnostic tests or even commercial ELISA kits. Thanks to its overall dimensions, ease of use and high-throughput analysis, the device can be used as a substrate in automated multi-well plate readers and improve the efficiency of conventional fluorescence immunoassays.