MICROPROPAGATION OF SOME SORT GROUPS OF LILIES

The article presents data on micropropagation of ten cultivars of the genus Lilium L. belonging to four groups: the Oriental hybrids, the Trumpet hybrids, Lilium Asiatic hybrids, the Longiflorum and Asiatic hybrids (LA-hybrids). It is found that for the sterilization of explants better use the 7% sodium hypo-chlorite solution (20 min) and 70% ethanol (10 sec). The average percentage of sterile culture for the Ori-ental hybrids was 66.00±7.07, the Trumpet hybrids – 81.00±5.65, Lilium Asiatic hybrids – 85.00±4.69, LA-hybrids – 87.00±6.36. There were no statistically significant differences between cultivars within the group (p=0.33, p<0.05). It is recommended to use a medium Murashige and Skuga with the addition of auxin α-naphthylacetic acid 1 ml/l – for the Oriental, Trumpet, Asiatic hybrids or cytokinin 6-benzylaminopurine 1 m/l – for LA hybrids. The percentage of explant with signs of regeneration was more than 82% for Trumpet and LA hybrids and more than 71% for Oriental and Asiatic hybrids. The reproduction rate can vary from 2 to 14 pcs/explant. Plant survival was more than 60%.

Analyses of asiatic hybrids resistance at introduction in northern forest steppe of west Siberia

The contents of main pigments of photosynthetic apparatus (chlorophyll a, b and carotinoid) in the leaves of Asiatic hybrids varieties during different stage of vegetation was first determined In conditions of northern forest steppe. It was established that 19 varieties of Asiatic hybrids had maximum level of accumulation of chlorophyll pigments (a and b) during the budding period, 4 – during the flowering period, 2 – during the fructification period; the most important value a/b was proper to 8 varieties in the budding period, 6 – in the fructification period and 11 in the flowering period, the maximum accumulation of carotinoind contained in the budding period that was expressed in the adaptive reaction of plants aimed to increase of photosynthetic apparatus resistance during the most dry season period. Analyses of photosynthetic pigment contents in the leaves of Asiatic hybrids varieties showed that the flowing changes in the contents of pigment complex may be considered as indicators of a mechanism of adaptation of lily varieties to the complex of climatic and natural conditions of northern forest steppe of West Siberia.

Variation in Floral Scent Compositions of Different Lily Hybrid Groups

Lilium cultivars have a wide range of variation in floral scent phenotypes. Using gas chromatography–mass spectrometry (GC/MS) analyses of volatile emissions during the night, the floral scent compositions of 35 lily cultivars from seven different hybrid groups were studied. The results showed that there was a positive correlation between volatile emission levels and scent intensities. Nonscented lily cultivars belonging to Asiatic hybrids hardly emitted volatiles, light-scented Longiflorum × Asiatic hybrids emitted low levels of volatiles, and scented lily cultivars (belonging to Oriental, Trumpet, Longiflorum, Longiflorum × Oriental, and Oriental × Trumpet hybrids) emitted significantly high levels of volatiles. In general, the scent compositions of lily cultivars were similar within the same hybrid group, and the differences among hybrid groups reflect their pedigree. Monoterpenoids and benzenoids dominated the floral scents of most volatile-emitting lily cultivars, whereas monoterpenoids alone dominated the floral scents of some volatile-emitting lilies. Although various scent compounds were released from volatile-emitting lily cultivars, the dominant scent compounds were focused on three monoterpenoids [1.8-cineole, linalool, and (E)-β-ocimene] and one benzenoid (methyl benzoate). The scent traits of lily cultivars could be traced back to their parents.