Influence of temperature processing on the constitutive and inducible resistance of rye seedlings to chloride salinization

We studied the growth rate of rye seedlings, as well as the dynamics of the content of soluble proteins and proline in the shoots during their adaptation to sharp (300 mM NaCl once, exposure time 9 days) and gradual (100 mM NaCl, then 100 mM NaCl after 2 days to the final concentration of 400 mM) salinity with sodium chloride in the presence or absence of thermal hardening (+40°C, 3 h). The established dy-namics of the content of proline and soluble proteins in the shoots suggests that the formation of re-sistance to salinity is determined by the high constitutive level of proline, as well as the stress-inducible synthesis of proline and water-soluble proteins. Thermal pretreatment of the seedlings stimulated their constitutive stability to a greater extent. The detected metabolic changes are obviously related to one of the possible mechanisms of the protective effect of thermal hardening on subsequent salinization.

Birds of the family Motacillidae in Omsk and in the Omsk province: species richness, numbers and distribution

The materials of year-round bird counts conducted by the authors in Omsk from September 2006 to Au-gust 2009 in nine habitats, as well as on the territory of four municipal districts of the Omsk Province from June 1 to August 10, 2020 have been analyzed. The species richness was studied, the analysis of the species numbers and distribution was carried out, and the abundance of Motacillidae on the territory of the studied administrative districts is indicated. Species sketches are given, in the compilation of which special attention is paid to the nature and duration of stay, as well as the distribution of birds by habitat. It was found that the encountered representatives of this family inhabit all studied biotopes, but some spe-cies are not evenly distributed.

Structure of spider-harvestman assemblages (Arachnida, Araneae, Opiliones) of model biotopes of Visimskiy natural reserve

The assemblages of herpetobion spiders from four habitats: two seven-year-old burns, spruce-birch and fir-spruce forests, were examined in the Visimskiy Reserve (Sverdlovsk Region) during May-September 2017 using pitfall-traps. The structure of the spiders population of burns significantly differs from that of forests in species composition, total occurrence rate (ind./100 trap-days), seasonal dynamics and composi-tion of the dominant species. Differences in the structure of the population are most pronounced in the summer. In summer spider assemblages of burns, the most abundant are Agyneta allosubtilis, Alopecosa pulverulenta, P. fulvipes, P. lugubris, P. riparia and Piratula hygrophila, in forests - Allomengea scopigera and Haplodrassus soerenseni.

Assessment of the state of populations of protected plants species in Russko-Polyansky municipal district of Omsk region

The results of monitoring the populations of protected plant species of the Omsk region in the territories of Russko-Polyansky municipal district are presented. During the research 210 cenopopulations of 32 of protected plants species were found in total. The locations of 20 protected species at the regional level were identified at the studied district for the first time: Adonis villosa, A. volgensis, Allium clathratum, Alyssum lenense, Astragalus buchtormensis, A. stenoceras, Dianthus ramosissimus, Ephedra distachya, Fritillaria meleagroides, Hedysarum gmelinii, Iris halophila, I. humilis, Linum perenne, Orostachys spinosa, Puccinellia gigantea, Ranunculus polyrhizos, Stipa lessingiana, Tanacetum millefolium, Tulipa patens, Valeriana tuberose. Two species (Stipa pennata, S. zalesskii) have considered as federal protected objects. Three sites were identified where the maximum number of protected species in natural habitats is concentrated, one of them has now received the status of a specially protected natural area of local and regional significance.

The fauna and benthic communities of invertebrates of the Ural river within the Atuar steppe (Orenburg state nature reserve)

There are 70 species and forms in the composition of zoobenthos of the river Ural and in water bodies of its floodplain have been identified. Mayflies and chironomids are represented in various ways (13 and 12 species and forms, respectively). Caddisflies (10) and stone flies (7) are rich in species. Among other invertebrates, bivalves (6) and gastropods (5), small-bristled worms (5), non-chironomid dipterans (4), leeches, crustaceans and dragonflies, bugs and beetles were recorded. On sandy-gravel-pebble soils is formed a zoobenthocenosis, dominated by the mayflies Ephoron virgo. They are accompanied by Chironomidae larvae. The biomass of the community varies between of 29.32–31.68 g/m2 with an abundance of 18.1–32.44 thousand ind./m2. In the coastal of the channel is formed a pelophilic zoobenthocenosis, the basis of it abundance and biomass are constituted by the small-bristle worms Limnodrilus hoffmeisteri. They are accompanied by mollusks Stagnicola palustris and mysids P. lacustris. The biomass of the pelophilic community is 13.68 g/m2 with a population of 15.45 thousand ind./m2. In floodplain water bodies are formed pelophilic zoobenthocenoses, the biomass of which varies in the range of 19.2–34.24 g/m2 with an abundance of 5.64–30.04 thousand ind./m2. Chironomid larvae constitute the base of the population. The biomass is composed by dragonflies Libellula sp., Chironomids, and mollusks Bithynia tentaculata.

New records of water beetles (Coleoptera) in the territories of Udmurtiya and Bashkiriya

This publication serves as a continuation of the inventory of aquatic beetle faunas in the Udmurtiya and Bashkortostan, a summary of which was published in 2020. The known composition of the faunas of the republics at the time of the study consisted of 230 (for Udmurtiya) and 120 (for Bashkiriya) species of aquatic beetles (including Donaciinae and Bagoiinae). The material for the article was the collections carried out in the framework of the study of the macrozoobenthos of rivers, oxbow lakes and temporary waterbidies from 2019 to 2020. Sampling of adults and beetle larvae from substrates submerged in water (stones and wood) was carried out manually. When collecting aquatic organisms in overgrown water bodies and rivers, a hydrobiological scraper was used. Data on the first finds of 5 species from 5 families in the territory of Udmurtiya and 6 species from 4 families in Bashkiriya are given. They are respectively as following: Orectochilus villosus (Gyrinidae), Haliplus confinis (Haliplidae), Rhantus bistriatus (Dytiscidae), Helophorus redtenbacheri (Helophoridae), Limnebius crinifer (Hydraenidae) in Udmurtiya, and Haliplus varius (Haliplidae), Ilybius fenestratus, Hydroporus palustris, Graptodytes bilineatus (Dytiscidae), Helophorus brevipalpis (Helophoridae), Limnebius cf. parvulus (Hydraenidae) in Bashkiriya.

Phylogenetic diversity of bacteria in rock salt of the Verkhnekamskoe deposit (Perm Krai)

New data on the phylogenetic diversity of bacteria in the salt of the Verkhnekamskoe deposit (Perm Krai) have been obtained using molecular genetic methods. Analysis of the 16S rRNA genes of total DNA from the rock salt sample (depth intervals of 239.7-239 m) evinces the presence of bacteria belonging to the classes: Actinobacteria (closely related to the genera Rhodococcus, Demequina), Gammaproteobacteria (the genera Pseudomonas, Serratia, Shigella), Betaproteobacteria (the genus Ralstonia) and Alphaproteobacteria (the genus Phyllobacterium). In addition, we identified two phylotypes of the Alphaproteobacteria (clone 66BA (GenBank MH410136) and clone 12BA (GenBank MH410128) that show lower similarity in 16S rRNA genes (98.46%) with the closest type strains of the genus Mesorhizobium (M. alhagi CCNWXJ12-2T) and the genus Chelativorans (C. multitrophicus DSM9103T). The identified phylotypes may represent new taxonomic units.

Spatial analysis of competition and cooperation of trees in pine forest crops

In forest cultures of scots pine 1 in bonita at the age of 55 years with a standing density of 940-1620 pcs./ha with a fullness of 0.84-1.02 on an area of 1.9 ha, places with low and medium density (5 sample areas, 433 trees) were selected to study the influence of microcenoses on the diameters of trees in their center. To do this, in the "ArcMap-ArcView" program, food polygons for the age of 30-40 years and 41-55 years were built around the trees. Five indicators were used in the spatial analysis of the data: 1 - simple pressure of neighbors: the diameters of the neighbors on the polygon were summed up; 2 - specific pressure of neighbors: indicator 1 was divided by the feeding area of the central tree; 3 - pressure of neighbors taking into account the distances to the central tree: the diameter of the neighbor was divided by the distance to the central tree and the data were summed; 4 - specific pressure of neighbors taking into account the distances: indicator 3 was divided by the feeding area of the central tree; 5 - cooperation of trees on the sides of the polygon: the diameter of the neighbor was multiplied by the contact distance with the central tree on the side of the polygon and the data were summed. It was not possible to choose the best indicator, since all five were weaker than the influence of a simple power supply area. When using the feeding area at the age of 30-40 years, and then at 41-55 years, the specific pressure of neighbors varied in strength from 5.8 to 8.9%, averaging 7.4%, and when taking into account the distances to neighbors, this pressure increased to an average of 8.5%. At an average density, the strength of the trees ' cooperation was equal to 11.6%, and at a low density of 7.5%, averaging 9.6%. Thus, the cooperation of trees in the microcenosis was generally 1.13 times stronger than the competitive pressure of neighboring trees

Diversity of culturable aerobic bacteria isolated from lindane-contaminated soils

It was found that in the soils sampled on the territory of OJSC "Sredne-Volzhsky plant of chemicals" in Chapaevsk, Samara region in 2013, the concentration of lindane varies in the range of 0.393 - 53.449 mg / kg of soil. 26 strains of aerobic culturable bacteria were isolated from soils. It was shown that there is a direct correlation between the level of contamination of the soil sample with lindane and the number of isolated strains. The dominant position is occupied by representatives of the phylum Proteobacteria, clas-ses α-, β- and γ-Proteobacteria. It was found that strains of Ochrobactrum sp. NE3 and Ochrobactrum sp. 132 decompose by-products of biotransformation of lindane (1,2,4-TCB and 2,5-DCP), and can be recommended for use in biotechnologies for remediation of lindane-contaminated soils to prevent the ac-cumulation of hazardous compounds.

Comparative evaluation of microbial contamination in and periodontitis and periimplantitis. Review

At the present stage of the development of dentistry, dental implantation occupies a leading place in the system of complex rehabilitation of patients with defects in the dentition. The success of dental implant placement depends on many factors. Among the significant reasons influencing the positive outcome of transplantation, the state of microbiocinosis of the oral cavity of patients should be noted. The perimplant zone is a favorable habitat for anaerobes. The pathological process in the form of periodontitis, which is often the reason for the loss of teeth and the placement of implants, contributes to the progression of the inflammatory process in the periodontal tissues. This article provides an overview of the scientific litera-ture devoted to the study of microflora in inflammatory periodontal diseases. We conducted a comparative assessment of the microbiota in periodontitis and peri-implantitis, which, according to individual authors, are different ecosystems in terms of the composition of microorganisms. Alternative views on this issue were considered. Based on the analyzed scientific data, we made a conclusion about the similarities and differences in the oral microbiota in periodontitis and peri-implantitis, as well as in their combined course. We substantiated the feasibility of assessing microbial pathogens in the peri-implant area in order to develop preventive and therapeutic measures aimed at the long-term survival of dental implants.