Biosensing Studies on Cuo-Mgo Nanocomposites for Glucose Detection

Approximately 3 million people around the world suffer from diabetes. One of the basic indications of an individual suffering from diabetes can be observed in the form of peaked levels of glucose in the blood. Thus, it is imperative for a non-invasive mechanism to be derived through which glucose levels in the blood can be detected throughout a regular time frame. The aim of this project focuses on synthesis of a nanocomposite, which can be used to detect glucose levels in the blood in a non-invasive manner. The selected nanocomposite was made from a mixture of CuO and MgO. Once synthesized, it was subjected to a series of tests and scans. The results demonstrated effective and efficient glucose detection activity of the CuO-MgO nanocomposite.

Nuclear Phosphatidylinositol-Phosphate Type I Kinase α-Coupled Star-PAP Polyadenylation Regulates Cell Invasion

ABSTRACTStar-PAP, a nuclear phosphatidylinositol (PI) signal-regulated poly(A) polymerase (PAP), couples with type I PI phosphate kinase α (PIPKIα) and controls gene expression. We show that Star-PAP and PIPKIα together regulate 3′-end processing and expression of pre-mRNAs encoding key anti-invasive factors (KISS1R,CDH1,NME1,CDH13,FEZ1, andWIF1) in breast cancer. Consistently, the endogenous Star-PAP level is negatively correlated with the cellular invasiveness of breast cancer cells. While silencing Star-PAP or PIPKIα increases cellular invasiveness in low-invasiveness MCF7 cells, Star-PAP overexpression decreases invasiveness in highly invasive MDA-MB-231 cells in a cellular Star-PAP level-dependent manner. However, expression of the PIPKIα-noninteracting Star-PAP mutant or the phosphodeficient Star-PAP (S6A mutant) has no effect on cellular invasiveness. These results strongly indicate that PIPKIα interaction and Star-PAP S6 phosphorylation are required for Star-PAP-mediated regulation of cancer cell invasion and give specificity to target anti-invasive gene expression. Our study establishes Star-PAP–PIPKIα-mediated 3′-end processing as a key anti-invasive mechanism in breast cancer.

Place and role of nonabsorbable antibiotics in the treatment of acute infectious diarrhea

Objectives. In the present study we wanted to evaluate the efficiency of Rifaximin-α on acute infectious diarrhea. Materials and methods. We revised the literature, as well as the studies which have been published within recent years. Discussions and conclusions. Rifaximin-α is an nonabsorbable antibiotic, bactericid, with a wide spectrum of action. It is recommended in acute diarrheic syndromes without invasive mechanism. Its properties (lack of intestinal absorbtion, minimal effect on colonic flora and no medication interactions) confer Rifaximin-α an excellent safety profile. Taking into account its wide spectrum of action and its high intestinal concentrations, it is considered an efficient and safe option in the treatment of acute infectious noninvasive diarrhea, as well as in the treatment and the prophilaxy of traveller´s diarrhea. Further more ample studies are needed to evaluate its efficiency in the treatment of recurrent infections with Clostridium difficile.

Association of Providencia alcalifacienswith Diarrhea in Children

It has been demonstrated in previous studies that Providencia alcalifaciens can produce diarrhea by an invasive mechanism. In the present study, P. alcalifaciens was isolated from the stool specimens of 17 of 814 diarrheal children younger than 5 years of age (2.1%) and from those of 4 of 814 matched controls (0.49%) (P = 0.004), indicating that the organism is significantly associated with diarrhea. However, 71% of P. alcalifaciens-positive diarrheal children had simultaneous infections with other recognized enteric pathogens.