Effect of the seed coat on dormancy and germination in Stylosanthes humilis H. B. K. Seeds

Seed of Townsville stylo (Stylosanthes humilis H.B.K.) is known to exhibit a hard seed coat and when freshly harvested also show a physiological dormancy, however, the nature of the co-actions between seed coat and embryo growth that determine dormancy is poorly understood. In this study, physical dormancy of Townsville stylo seeds was not reduced during natural ageing at room temperature, in contrast to the physiological dormancy, which is gradually overcome during after-ripening. Furthermore, the permeability of seed coat was affected by scarification treatments as well as by low-pH solutions. Together, these data indicate that physical dormancy overcome of seed is prerequisite for radicle protrusion and physiological dormancy of Townsville stylo seeds contribute to its timing.

Dormancy breakage of Stylosanthes humilis seeds by aluminium

Physiological dormancy of scarified seeds of Townsville stylo (Stylosanthes humilis HBK) was released by acidic aluminium (Al3+) solution. Antiethylenic substances inhibited germination of low-pH-stimulated dormant seeds, with a correspondingly low ethylene production and low activity of 1-aminocyclopropane-1-carboxylate (ACC) oxidase in seeds. On the other hand, antiethylenic substances did not decrease the germination of Al3+-stimulated seeds, but ACC oxidase activity and ethylene production by the seeds was decreased to a large extent. These data provide evidence that dormancy breakage by Al3+ differs from that caused by low pH and is not associated with ethylene production. Similarly to Al3+ action, methyl viologen (MV), a reactive oxygen species-generating compound, broke dormancy of Townsville stylo seeds. Sodium selenate and N-acetyl cysteine, antioxidant compounds, largely decreased germination of MV- and Al3+-stimulated dormant seeds. Altogether these data point to oxidative radicals constituting key molecules in the chain of events triggered by Al3+ leading to dormancy breakage.

Sensitivity to ethylene as a major component in the germination of seeds of Stylosanthes humilis

Physiological dormancy of scarified seeds of Townsville stylo (Stylosanthes humilisH.B.K.) is broken by ethylene. When the biosynthesis of this gas was impaired by 2-aminoethoxyvinylglycine (AVG) plus Co2+, the response to ethylene at very low concentrations was appreciable in non-dormant seeds and nil in the dormant ones. Complete inhibition of germination of non-dormant seeds occurred only when they were treated with AVG plus Co2+under an ethylene-free atmosphere, a condition in which no trace of the gas in the atmosphere of Erlenmeyer flasks could be detected. Injection of ethylene into that system triggered germination of both dormant and non-dormant seeds, demonstrating a requirement for the gas. Non-dormant seeds were at least 50-fold more sensitive to ethylene than the dormant ones. Perception of ethylene occurred within a very short time (at most 15 min), since exposure of both dormant and non-dormant seeds to the gas, at a steeply declining concentration, sufficed to cause substantial germination.

Germination of dormant seeds of Stylosanthes humilis as promoted by ethylene accumulation in closed environments

Germination of scarified dormant seeds of Townsville stylo (Stylosanthes humilis) is very low in Petri dishes, but may be appreciable in sealed Erlenmeyer flasks if ethylene accumulates in their atmospheres. When ethylene in the flask atmosphere was fixed by a mercuric perchlorate solution, emanation of the gaseous regulator and germination of dormant seeds were drastically decreased. On the other hand, ethylene produced by dormant seeds and accumulated in the flask atmosphere led to a further enhancement of ethylene accumulation and a corresponding increased seed germination. Germination of both dormant and non-dormant seeds did not occur when ethylene biosynthesis was completely blocked.

Management options for increasing the productivity of tropical savanna pastures. IV. Population biology of introduced Stylosanthes spp

In a large-scale grazing experiment in the Monsoon Tallgrass savannas of the Northern Territory, Townsville stylo (Stylosanthes humilis), Caribbean stylo (S. hamata) and perennial stylo (S. scabra + S. viscosa) were sown into native pasture and grazed at three stocking rates, under two fertilizer rates and with trees killed and unkilled. These treatments, together with the occurrence of anthracnose, led to the rapid decline in both yield and numbers of plants in those pastures sown to Townsville stylo. In contrast, yields of Caribbean stylo and Perennial stylo stabilized after three years in both fertilized and unfertilized swards. While total legume yield of these latter two treatments was similar, the dominant contribution in Caribbean stylo was made by annual plants, while in Perennial stylo swards individuals more than 18 months old contributed 80% of the yield.There were marked differences between all species in their population biology. Townsville stylo declined in numbers with death of individuals prior to seed set and the rapid depletion of the soil seed pool which had little long-lived seed. In contrast, in swards of Caribbean stylo a large amount of hard seed rapidly built up in the soil seed pool and ensured continuity of productivity even if seed was not set in some years. In the perennial stylos, seed softened quickly, and the soil seed pool remained small and short-lived. In these latter Stylosanthes spp., long-term stability depended on the survival of perennating plants rather than seed.Pasture management must take into account these different patterns of population dynamics. In particular, while fire is a potentially useful tool in renovating Caribbean stylo swards with their large soil seed pool, it can be extremely detrimental to perennial stylo swards where individuals are susceptible to burning and there is little soil seed available to re-establish the sward.

Evaluation of management options for increasing the productivity of tropical savanna pastures. 2. Legume species

The effect of legume species, used for augmentation of native perennial grasses, upon forage production and quality and animal production was studied over 4 vears in the semi-arid troPics of northwestern Australia. The legumes were Stylosanthes humilis cv. Paterson (Townsville stylo), S. hamata cv. Verano (Caribbean stylo) and a mixture of perennial species S. scabra cvv. Fitzroy and Seca and S. viscosa (perennial stylo). They were compared at 2 levels of fertility, unfertilised (UF) and with small inputs of superphosphate (F); and at stocking rates of 0.45,0.6 and 0.75 steers/ha for UF and 0.6, 0.8 and 1.0 steers/ha for F. By 1980 only half of the 3 x 12 treatments persisted, 3 Townsville stylo, 9 Caribbean stylo and 6 perennial stylo. Without fertiliser the perennial stylos had the highest wet season yields of 1-2 t/ha which constituted 4040% of pasture yield, compared with 2-20% for the other legumes. Caribbean stylo yields were highest with fertiliser, yielding 2-4 t/ha and 50-90% of the pasture at the end of the wet season, compared with 60-70% for perennial stylo and 5-30% for Townsville stylo. Anthracnose devastated Townsville stylo, and also reduced the productivity of Fitzroy. The perennial stylos had the highest concentrations of nitrogen (N), phosphorus (P), and sulfur (S) during the dry season while Caribbean stylo and its associated grasses had the lowest P and S concentrations during the wet season. When annual liveweight gains for each legume were compared at the same stocking rate-fertiliser level, differences were less than 15 kg, but there were considerable differences in the patterns of growth within the year; these differences were related to the seasonal availability and relative palatability of grass and legume. The overall mediocre animal growth of 30-110 kg/steer.year was attributed to the inadequate supply of nutrients for most of the year, with the possible exception of N.

Improvement in soil structure resulting from sown pastures on degraded areas in the dry savanna woodlands of northern Australia

The structure of the surface soil under pastures sown on a former Themada australis native grassland and on degraded areas in the red earths of northern Australia was examined micromorphologically. Heavily grazed pastures containing the legumes Stylosanthes hamata cv. Verano and Alysicarpus vaginalis had more macropore space in the surface soil than arlightly grazed native grassland, while pastures containing S. humilis (Townsville stylo) had as little macropore space as degraded areas. Macropore space in a S. hamata pasture increased between the third and fourth wet seasons after establishment. Where macropore space was high, infiltration measurements showed that sorptivities were as high as those in the native grassland. Where macropore space was low, sorptivities were as low as those in degraded areas. However, all sown pastures showed low hydraulic conductivities equivalent to those in the degraded areas, and this was attributed to trampling during the wet season under the higher stocking rates involved. The improvement and maintenance of soil structure in the S. hamata and A. vaginalis pastures, together with their high sorptivities, indicates that they are stable in the long term.

Effect of superphosphate application on the phosphorus status of breeding cattle grazing Townsville stylo based pastures in northern Queensland

The performance of Brahman crossbred cows grazing annual grass plus Townsville stylo (Stylosanthes humils) pasture with or without superphosphate fertilizer was monitored over a two year period. The animals were introduced to the experimental areas as yearling helfers and remained until weaning of their first calf. Levels of phosphorus In rib bone varied between 145 and 172 mg/ml. At weanlng, levels were higher on the fertilized than on the unfertilized pasture (172 vs. 157 mg/ml). Calcium levels in rib bone varied between 329 and 362 rng/ml and paralleled bone phosphorus levels. Pasture nitrogen levels were similar on both pastures but phosphorus levels were higher on the fertilized area. Faecal analyses reflected this same pattern with faecal nitrogen levels being similar on both pastures, and faecal phosphorus levels higher on the fertilized pasture. Liveweight changes of cows and calf weaning weights were greater in groups grazing the fertilized pasture. It is hypothesized that this superior performance is a function of increased feed intake resulting from increased phosphorus intake on the fertilized area although the possible contribution of sulfur cannot be overlooked.