Remobilizing deleted piggyBac vector post-integration for transgene stability in silkworm

2015 ◽  
Vol 290 (3) ◽  
pp. 1181-1189 ◽  
Author(s):  
Feng Wang ◽  
Riyuan Wang ◽  
Yuancheng Wang ◽  
Hanfu Xu ◽  
Lin Yuan ◽  
...  
Keyword(s):  
Transgene Stability ◽  
Piggybac Vector

Genetically manipulated pineapple: transgene stability, gene expression and herbicide tolerance under field conditions

2006 ◽  
Vol 125 (4) ◽  
pp. 411-413 ◽  
Author(s):  
S. Sripaoraya ◽  
S. Keawsompong ◽  
P. Insupa ◽  
J. B. Power ◽  
M. R. Davey ◽  
...  
Keyword(s):  
Gene Expression ◽  
Herbicide Tolerance ◽  
Field Conditions ◽  
Transgene Stability ◽  
Genetically Manipulated

Transgene stability and features of rasH2 mice as an animal model for short-term carcinogenicity testing

2002 ◽  
Vol 34 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Hiroshi Suemizu ◽  
Kaori Muguruma ◽  
Chika Maruyama ◽  
Masashi Tomisawa ◽  
Minoru Kimura ◽  
...  
Keyword(s):  
Animal Model ◽  
Short Term ◽  
Transgene Stability ◽  
Rash2 Mice

scholarly journals Direct conversion of human pluripotent stem cells into cranial motor neurons using a piggyBac vector

2018 ◽  
Vol 29 ◽  
pp. 189-196 ◽  
Author(s):  
Riccardo De Santis ◽  
Maria Giovanna Garone ◽  
Francesca Pagani ◽  
Valeria de Turris ◽  
Silvia Di Angelantonio ◽  
...  
Keyword(s):  
Stem Cells ◽  
Motor Neurons ◽  
Pluripotent Stem Cells ◽  
Direct Conversion ◽  
Human Pluripotent Stem Cells ◽  
Piggybac Vector

Germ-line transformation of the Queensland fruit fly, Bactrocera tryoni, using a piggyBac vector in the presence of endogenous piggyBac elements

Genetica ◽  
2010 ◽  
Vol 139 (1) ◽  
pp. 91-97 ◽  
Author(s):  
K. A. Raphael ◽  
D. C. A. Shearman ◽  
K. Streamer ◽  
J. L. Morrow ◽  
A. M. Handler ◽  
...  
Keyword(s):  
Germ Line ◽  
Fruit Fly ◽  
Bactrocera Tryoni ◽  
Piggybac Vector ◽  
Germ Line Transformation

scholarly journals Pogostick: A New Versatile piggyBac Vector for Inducible Gene Over-Expression and Down-Regulation in Emerging Model Systems

PLoS ONE ◽  
2011 ◽  
Vol 6 (4) ◽  
pp. e18659 ◽  
Author(s):  
Bin Chen ◽  
Steven Hrycaj ◽  
Johannes B. Schinko ◽  
Ondrej Podlaha ◽  
Ernst A. Wimmer ◽  
...  
Keyword(s):  
Model Systems ◽  
Down Regulation ◽  
Over Expression ◽  
Piggybac Vector ◽  
Inducible Gene

Stably Express Spider Flagelliform Silk Protein in Bombyx Mori Cell Line by PiggyBac Transposon-Derived Vector

2011 ◽  
Vol 332-334 ◽  
pp. 779-782 ◽  
Author(s):  
Yuan Song Zhang ◽  
Tian Fu Zhao ◽  
Ai Chun Zhao ◽  
Masao Nakagaki
Keyword(s):  
Cell Line ◽  
Blot Analysis ◽  
Spider Silk ◽  
Silk Protein ◽  
Transgenic Silkworm ◽  
Helper Plasmid ◽  
Antibiotic Selection ◽  
Spider Silk Protein ◽  
Piggybac Vector ◽  
Turn Structure

Silkworm BmN4 cells were transfected with the helper plasmid and the piggyBac vector( piggyBac-FLAG) in which was inserted with the spider flagelliform silk expression cassette. Via antibiotic selection, most cells showed stable DsRed-expression. Immuno blot analysis showed that flagelliform silk protein of spider was expressed stably in BmN4 cells. Circular dichroism spectra indicated the existence of β-turn structure in recombinant spider flagelliform protein. The present results suggested that transgenic silkworm directly secreting functional spider silk protein in cocoon by using piggyBac system is feasible.

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