Evaluation of productivity and costs of Malwa forest machine in sanitary fellings in Latvia

The topicality of the study is determined by the increasing demand for sanitary felling services currently mainly provided by manual work. Harvesting using hand-held motor instruments is a physically hard work whose costs are increasing rapidly, but labor availability is declining. In sanitary cuttings, additional complications are caused by the use of conventional machinery in harvesting, which necessitates the creation of wide (4 m) technological corridors and significantly increases the proportion of damaged remaining trees in a stand (according to past studies, a set of middle-class forest machines in thinning of spruce stands results in 4–5% of damaged trees, but in pine stands – in approximately twice less damaged trees than in spruce stands). The compact class forest machines in thinnings result in mechanical damage to not more than 1% of remaining trees. During field trials in sanitary felling 9329 trees were felled. The average productivity working in one shift is 5.35 m3 h-1 with average stands 10 cm. The cost of harvester’s productive hour is 92 € but of a forwarder it is 78. The compact class forwarder creates significantly smaller impact on the soil by reducing ruts depth and soil compaction, which is especially important in sanitary fillings and extraction of seed trees in regenerated areas.

SIMULATION OF PULSATILE FLOW THROUGH ARTERIAL TREE MODELS

In this work, an analytical scheme for evaluating the local wave characteristics of flow and pressure in 1D arterial tree’s models is implemented. An interactive computational environment was developed to simulate this scheme. The presented environment contains a compact class structure to enable the iteration and visualization of multiple objects. The results obtained are consistent with those from literature. The software developed provides the visualization of a blood flow simulation through a simple user interface.

Characterization and applications of Type I CRISPR-Cas systems

CRISPR-Cas constitutes the adaptive immune system of bacteria and archaea. This RNA-mediated sequence-specific recognition and targeting machinery has been used broadly for diverse applications in a wide range of organisms across the tree of life. The compact class 2 systems, that hinge on a single Cas effector nuclease have been harnessed for genome editing, transcriptional regulation, detection, imaging and other applications, in different research areas. However, most of the CRISPR-Cas systems belong to class 1, and the molecular machinery of the most widespread and diverse Type I systems afford tremendous opportunities for a broad range of applications. These highly abundant systems rely on a multi-protein effector complex, the CRISPR associated complex for antiviral defense (Cascade), which drives DNA targeting and cleavage. The complexity of these systems has somewhat hindered their widespread usage, but the pool of thousands of diverse Type I CRISPR-Cas systems opens new avenues for CRISPR-based applications in bacteria, archaea and eukaryotes. Here, we describe the features and mechanism of action of Type I CRISPR-Cas systems, illustrate how endogenous systems can be reprogrammed to target the host genome and perform genome editing and transcriptional regulation by co-delivering a minimal CRISPR array together with a repair template. Moreover, we discuss how these systems can also be used in eukaryotes. This review provides a framework for expanding the CRISPR toolbox, and repurposing the most abundant CRISPR-Cas systems for a wide range of applications.