scholarly journals The Bradyrhizobium Sp. LmicA16 Type VI Secretion System Is Required for Efficient Nodulation of Lupinus Spp.

2021 ◽  
Author(s):  
L. Tighilt ◽  
F. Boulila ◽  
B. F. S. De Sousa ◽  
E. Giraud ◽  
T. Ruiz-Argüeso ◽  
...  
Keyword(s):  
Secretion System ◽  
Type Vi Secretion System ◽  
Type Vi Secretion ◽  
Bradyrhizobium Sp

scholarly journals The Bradyrhizobium Sp. LmicA16 Type VI Secretion System is Required for Efficient Nodulation of Lupinus Spp.

2021 ◽  
Author(s):  
Tighilt L. ◽  
Boulila F. ◽  
De Sousa BFS ◽  
Giraud E ◽  
Ruiz-Argüeso T ◽  
...  
Keyword(s):  
Wild Type Strain ◽  
Gene Clusters ◽  
Secretion System ◽  
Type Vi Secretion System ◽  
Free Living ◽  
Type Vi Secretion ◽  
Divergent Gene ◽  
Bradyrhizobium Sp ◽  
Symbiotic Phenotype ◽  
Free Living Conditions

Abstract Many bacteria of the genus Bradyrhizobium are capable of inducing nodules in legumes. In this work, the importance of a type VI secretion system (T6SS) in a symbiotic strain of the genus Bradyrhizobium is described. T6SS of Bradyrhizobium sp. LmicA16 (A16) is necessary for efficient nodulation with Lupinus micranthus and L. angustifolius . A mutant in the gene vgrG, coding for a component of the T6SS nanostructure, induced less nodules and smaller plants than the wild type strain (wt) and was less competitive when co-inoculated with the wt strain. A16 T6SS genes are organized in a 26 kb DNA region in two divergent gene clusters of nine genes each. One of these genes codes for a protein (Tsb1) of unknown function but containing a methyltransferase domain. A tsb1 mutant showed an intermediate symbiotic phenotype regarding vgrG mutant and higher mucoidy and motility than the wt strain in free living conditions. T6SS promoter fusions to the lacZ reporter indicate expression in nodules but not in free living cells grown in different media and conditions. The analysis of nodule structure revealed that the level of nodule colonization was significantly reduced in the mutants with respect to the wt strain.

scholarly journals Clinical impact of the type VI secretion system on virulence of Campylobacter species during infection

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Jessica Agnetti ◽  
Helena M. B. Seth-Smith ◽  
Sebastian Ursich ◽  
Josiane Reist ◽  
Marek Basler ◽  
...  
Keyword(s):  
Secretion System ◽  
Clinical Impact ◽  
Type Vi Secretion System ◽  
Type Vi Secretion ◽  
Campylobacter Species

scholarly journals VgrG and PAAR Proteins Define Distinct Versions of a Functional Type VI Secretion System

2016 ◽  
Vol 12 (6) ◽  
pp. e1005735 ◽  
Author(s):  
Francesca R. Cianfanelli ◽  
Juliana Alcoforado Diniz ◽  
Manman Guo ◽  
Virginia De Cesare ◽  
Matthias Trost ◽  
...  
Keyword(s):  
Secretion System ◽  
Functional Type ◽  
Type Vi Secretion System ◽  
Type Vi Secretion

scholarly journals Structure of the type VI secretion system TssK–TssF–TssG baseplate subcomplex revealed by cryo-electron microscopy

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Young-Jun Park ◽  
Kaitlyn D. Lacourse ◽  
Christian Cambillau ◽  
Frank DiMaio ◽  
Joseph D. Mougous ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Secretion System ◽  
Type Vi Secretion System ◽  
Type Vi Secretion ◽  
Cryo Electron Microscopy

scholarly journals Expression of the Type VI Secretion System 1 Component Hcp1 Is Indirectly Repressed by OpaR inVibrio parahaemolyticus

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Lizhi Ma ◽  
Yiquan Zhang ◽  
Xiaojuan Yan ◽  
Liping Guo ◽  
Li Wang ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Stress Responses ◽  
Structural Component ◽  
Vibrio Parahaemolyticus ◽  
Secretion System ◽  
Upstream Region ◽  
Bacterial Protein ◽  
Type Vi Secretion System ◽  
Type Vi Secretion ◽  
System 1

The type VI secretion system (T6SS) is bacterial protein injection machinery with roles in virulence, symbiosis, interbacterial interaction, antipathogenesis, and environmental stress responses. There are two T6SS loci, T6SS1 and T6SS2, in the two chromosomes ofVibrio parahaemolyticus, respectively. This work disclosed that the master quorum sensing (QS) regulator OpaR repressed the transcription ofhcp1encoding the structural component Hcp1 of T6SS1 inV. parahaemolyticus, indicating that QS had a negative regulatory action on T6SS1. A singleσ54-dependent promoter was transcribed forhcp1inV. parahaemolyticus, and its activity was repressed by the OpaR regulator. Since the OpaR protein could not bind to the upstream region ofhcp1, OpaR would repress the transcription ofhcp1in an indirect manner.

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