scholarly journals Identificación de rizobios en nódulos de Lupinus alopecuroides Desr. desarrolladas en las chacras de Castrovirreyna, Huancavelica

Qantu Yachay ◽  
2021 ◽  
Vol 1 (1) ◽  
pp. 36-45
Author(s):  
Viane Marytrini Mejia Huaranca ◽  
Gregorio José Arone Gaspar
Keyword(s):  
16S Rrna ◽  
Bradyrhizobium Sp ◽  
Rhizobium Sp

Una de las leguminosas nativas de la región Puna y Janca de nuestro país es Lupinus alopecuroides Desr, esta leguminosa posee rol importante como planta pionera en colonizar espacios de suelos poco evolucionados y una vez establecida contribuye a regenerar la productividad natural de las chacras mediante la fijación biológica del N. Asimismo, los campesinos lo emplean en diversas actividades, en la salud de las personas y animales y para generar energía calorífica (leña). Dada su importancia se planteó analizar el endosimbionte de nódulos radicales de L. alopecuroides Desr desarrolladas en las chacras de Choccra Pucro (3996 m.s.n.m.), Castrovireyna, Huancavelica. Se evaluó 60 nódulos procedente de 20 plantas, se tomaron 3 nódulos por planta utilizando muestreo simple-aleatorio y se cultivaron en medio YEM. Lo que permitió obtener 30 aislados, cuando se amplificaron y secuenciaron el gen 16S rRNA se reagruparon en 14 géneros, 03 de ellos pertenecen a las rizobiaceas (Rhizobium sp., Bradyrhizobium sp. y Phyllobacterium sp.), y una vez inoculadas empleando plántulas de L. alopecuroides, el género Bradyrhizobium logró formar protuberancias de nódulos radicales. Asimismo, en los nódulos viven otros géneros no rizobianos como Erwinia, Rahnella, Serratia, Rhodococcus, Arthrobacter, Paenibacillus, Pseudomonas, Stenotrophomonas, Bacillus, Staphylococcus y Pantoea, cuya función queda por aclarar.

scholarly journals Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains

2010 ◽  
Vol 53 (5) ◽  
pp. 1185-1195
Author(s):  
Arlem Nascimento de Oliveira ◽  
Luiz Antonio de Oliveira ◽  
Jerusa Souza Andrade
Keyword(s):  
Nitrogen Sources ◽  
Serine Protease Inhibitor ◽  
Alkaline Proteases ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Crude Extracts ◽  
Bradyrhizobium Sp ◽  
Triton X ◽  
Acetone Hexane ◽  
Rhizobium Sp

Two rhizobia strains isolated from soils of the Central Amazonian floodplain produced appreciable quantities of crude alkaline protease extracts with inexpensive carbon and nitrogen sources. These protease crude extracts were optimally active at pH 9.0-11.0. The optimum temperatures were 35 ºC for Rhizobium sp. strain R-986 and 55 ºC for Bradyrhizobium sp. strain R-993. Protease activities in the crude extracts were enhanced in the presence of 5 mM metal ions, such as Na+, Ca2+, Mg2+ and Mn2+. Rhizobia proteases were strongly inhibited by PMSF, a serine-protease inhibitor. The enzymes were active in the presence of surfactants (SDS and Triton X-100) and stable in oxidizing (H2O2) and reducing agents (β-mercaptoethanol), and organic solvents (acetone, hexane, methanol, 1-propanol and toluene).

scholarly journals Effect of symbiotic association of rhizobia and endomycorrhizae from Moroccan arid littoral dunes on Acacia cyanophylla tolerance to drought

2018 ◽  
Vol 2 (2) ◽  
pp. 39-45
Author(s):  
ABDELHAKIM HATIMI ◽  
SAIDIA TAHROUCH ◽  
BRAHIM BOUIZGARNE
Keyword(s):  
Drought Stress ◽  
Water Content ◽  
High Efficiency ◽  
Stress Conditions ◽  
Symbiotic Association ◽  
Positive Effects ◽  
Acacia Cyanophylla ◽  
Growth And Nutrition ◽  
Bradyrhizobium Sp ◽  
Rhizobium Sp

Hatimi A, Tahrouch S, Bouizgarne B. 2018. Effect of symbiotic association of rhizobia and endomycorrhizae from Moroccan arid littoral dunes on Acacia cyanophylla tolerance to drought. Asian J For 2: 39-45. The research on behavior of A. cyanophylla Lindl plants associated with a symbiotic indigenous endomycorrhizal fungi M, and three rhizobia isolates: two low growing isolate R1 (Bradyrhizobium sp. RCM6), and R2 (Bradyrhizobium sp. RLC3) and a fast-growing isolates R3 (Rhizobium sp. S21), originated from coastal dunes of the Souss-Massa region in drought stress conditions, was investigated in greenhouse. Results have clearly shown that the growth and nutrition of seedlings of A. cyanophylla were drastically affected after two months in drought stress conditions. However, inoculation of the symbiotic microorganisms either alone (treatments M, RMC6, R2 or R3) or as inoculums consisting of combination of the rhizobia with the endomycorrhiza (treatments MR1, MR2 or MR3) resulted in enhanced tolerance of A. cyanophylla seedlings to drought stress. At 100% of field capacity (fc), all treatments showed a significant improvement of plant growth compared to non-inoculated plants in stress conditions. In addition, we have shown that Bradyrhizobium RCM6 (R1) holds a high efficiency to improve the growth and nutrition of the host plant. Indeed, higher number of nodules/plant and higher amount of total nitrogen were recorded in the seedlings inoculated with Bradyrhizobium sp. RCM6 in comparison with plants inoculated with the two other rhizobia Bradyrhizobium sp. RLC3 (R2) and Rhizobium sp. S21 (R3), and control plants. Dual inoculation with each of the three rhizobia and the endomycorrhizal complex (M) led to higher water content (W.C) and relative water content (RWC) and a significant increase in Phosphorus content of the aerial part. While positive effects were recorded for Phosphorus, no such effects were recorded for nitrogen. However, the overall results showed the importance of the use of microorganisms in the dune coastal environment particularly adequate tripartite association: rhizobia Endomycorrhizes-A. cyanophylla in enhancing tolerance to drought stress.

scholarly journals THE BIOLOGICAL PROPERTIES OF SOYBEAN NODULE BACTERIA WITH THE DIFFERENT SPEED OF GROWTH

2010 ◽  
Vol 10 ◽  
pp. 50-64
Author(s):  
D.V. Krutуlo
Keyword(s):  
16S Rrna ◽  
Biological Properties ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Soybean Nodule ◽  
Bradyrhizobium Sp ◽  
The One ◽  
Symbiotic Properties ◽  
Slow Growing ◽  
Intensive Growth

The phenotypical and genotypic properties of soybean microsymbionts with the different speed of growth were studied. It was established that the strains with the intensive growth were specific to the host-plant, but had differed by their serological, chemotaxonomical and symbiotic properties. The investigated intensive-growing strains were combined into the one serogroup by their antigenic content. The similarity of fatty acids spectrum was observed for the strains with the intensive growth Bradyrhizobium sp. КВ1-1, slow-growing strains B. japonicum 634b and B. japonicum КС2-3. The quantitative and qualitative differences in monosaccharides contents of EPS of the studied strains were revealed. Presence of xylose (2,7 %) and increased contents of rhamnose (18,4 %) were shown to be different in EPS of strain Bradyrhizobium sp. КВ1- 1 and EPS of strains B. japonicum КС2-3 and B. japonicum 634b. Analysis of the sequences of the 16S rRNA genes allowed attributing the strain with the intensive growth to the Bradyrhizobium genus. Genes consistency analysis of 16S rRNA had allowed to refer the intensivegrowing strains to the Bradyrhizobium genus. It was shown that studied intensive-growing strains had formed the nonspecific symbiosis with cow pea.

scholarly journals Response of Cratylia argentea (Desvaux) O. Kuntzeto inoculation with Rhizobium sp. and Bradyrhizobium sp. strains

2018 ◽  
Vol 12 (06) ◽  
pp. 849-854
Author(s):  
Eduardo P. L. Mattar ◽  
◽  
Tiago T. V. Barros ◽  
Bruno P. Brasileiro ◽  
Edson M. Mattielo ◽  
...  
Keyword(s):  
Bradyrhizobium Sp ◽  
Rhizobium Sp

Biological Nitrogen Fixation and Root-Nodule Bacteria (Rhizobium Sp. and Bradyrhizobium Sp.) In Two Rehabilitating Sand Dune Areas Planted With Acacia Spp

1985 ◽  
Vol 33 (5) ◽  
pp. 595 ◽  
Author(s):  
YM Barnet ◽  
PC Catt ◽  
DH Hearne
Keyword(s):  
Nitrogen Fixation ◽  
Biological Nitrogen Fixation ◽  
Sand Dune ◽  
Root Nodule ◽  
Nodule Bacteria ◽  
Root Nodule Bacteria ◽  
Bradyrhizobium Sp ◽  
Biological Nitrogen ◽  
Slow Growing ◽  
Rhizobium Sp

This paper reports a study of biological nitrogen fixation in two sand dune regions of New South Wales where planted Acacia spp. had been used in revegetation programmes. At one location (Bridge Hill Ridge), natural regrowth had produced a complex plant community, and native legumes in addition to the planted acacias were present. The other area (Wanda Beach) was a grossly disturbed site which contained only the planted species. Symbiotic fixation in association with Australian legumes occurred at both locations at rates within the range reported by other authors. Distinct seasonal changes were apparent, with higher activities in the cooler months. The legume association seemed the only source of biologically fixed nitrogen at Bridge Hill Ridge, but at Wanda Beach cyanobacteria in an algal mat also made a contribution. Fast and slow-growing bacterial strains were obtained from root nodules of native legumes at both sites and were classed as Rhizobium sp. and Bradyrhizobium sp., respectively. This division was supported by the pattern of serological affinities of the isolates and by differences in their protein profiles demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Two atypical types of root-nodule bacteria were found at Bridge Hill Ridge: non-nodulating, fast-growing isolates and an abnormally slow-growing Bradyrhizobium sp.

Phylogenetic analyses of Bradyrhizobium strains nodulating soybean (Glycine max) in Thailand with reference to the USDA strains of Bradyrhizobium

1999 ◽  
Vol 45 (8) ◽  
pp. 639-645 ◽  
Author(s):  
Shotaro Ando ◽  
Tadashi Yokoyama
Keyword(s):  
Glycine Max ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Phylogenetic Analyses ◽  
Rflp Analysis ◽  
Rrna Gene ◽  
Gene Probe ◽  
Close Relationship ◽  
Bradyrhizobium Sp

To elucidate the phylogenetic relationships between Thai soybean bradyrhizobia and USDA strains of Bradyrhizobium, restriction fragment length polymorphism (RFLP) analysis using the nifDK gene probe and sequencing of the partial 16S rRNA gene were performed. In our previous work, Thai isolates of Bradyrhizobium sp. (Glycine max) were separated clearly from Bradyrhizobium japonicum and Bradyrhizobium elkanii based on the RFLP analysis using the nodDYABC gene probe. RFLP analysis using the nifDK gene probe divided 14 Thai isolates and eight USDA strains of B. japonicum into different groups, respectively, but categorized into the same cluster. All of seven strains within these Thai isolates had the same sequence of the partial 16S rRNA gene, and it was an intermediate sequence between those of B. japonicum USDA 110 and B. elkanii USDA 76T. Furthermore, three USDA strains of B. japonicum, USDA 6 (B. japonicum ATCC 10324T), USDA 115 and USDA 129, had the same partial 16S rRNA gene sequence that seven Thai isolates had. These results suggest that Thai isolates of Bradyrhizobium sp. (Glycine max) are genetically distinct from USDA strains of B. japonicum and B. elkanii, but also indicate a close relationship between Thai isolates and USDA strains of B. japonicum.Key words: Bradyrhizobium, 16S rRNA gene, nif gene, soybean, Thailand.

scholarly journals Evaluation of Microbial Loads and Physico-Chemicals of Cassava Mill Effluent Simulated Soil

2021 ◽  
pp. 13-26
Author(s):  
Bassey Etta Agbo ◽  
Daniel Offiong Etim ◽  
Alfred Young Itah ◽  
Akan A. Brooks
Keyword(s):  
Bacterial Group ◽  
Nitrogen Fixing ◽  
Blue Green Algae ◽  
Bradyrhizobium Sp ◽  
Calopogonium Mucunoides ◽  
Anabaena Sp ◽  
Fusarium Sp ◽  
Corynebacterium Sp ◽  
Microbial Isolates ◽  
Rhizobium Sp

Evaluation of microbial loads and physico-chemicals of cassava mill effluent simulated soil was carried out using standard microbiological and biochemical techniques. This was to determine the effect of cassava mill effluent (CME) on rhizosphere microbial loads, physicochemical properties, nitrogenous salt and heavy metals. The results showed that CME effect on the physicochemical determinants (pH, Ca, Mg, K) and heavy metal determinant (Fe, Zn, Co, Ni, Pb and Mn) was concentration dependents. The nitrogenous salts (NO3, NH4+ and NO2) levels progressively increased with no significant differences (p>0.05 ANOVA). The microbial isolates were: Saccharomyces sp, Mucorindicus, Fusarium sp and Gliocladium sp for the fungal group. The bacterial group were Chromobacterium sp, Corynebacterium sp, Bacillus sp, Acinetobacter sp and Escherichia coli while the nitrogen-fixing bacterial group were Azotobacter sp., Azospirillum sp., Frankia sp., Bradyrhizobium sp., Hebaspirillum sp., Cyanobacteria (or blue green algae), Anabaena sp, Nostoc sp., Clostridium sp. and Rhizobium sp. There was no significant differences (p>0.05) in the rhizosphere microbial load across the concentration gradient at the CME-simulated plot phyto-remediated by Centrosema pubesscens and Calopogonium mucunoides. Agricultural wastes such as cassava mill effluent should be properly treated before discharging to the environment in other to prevent the loss of nitrogen-fixing bacteria and total heterotrophic bacterial genera that could be of immense importance to man.

Protein-induced conformational changes in 16S rRNA during assembly of the Escherichia Coli 30S ribosomal subunit: A STEM study

1987 ◽  
Vol 45 ◽  
pp. 910-911
Author(s):  
M. Boublik ◽  
V. Mandiyan ◽  
J.F. Hainfeld ◽  
J.S. Wall
Keyword(s):  
16S Rrna ◽  
Conformational Changes ◽  
Ribosomal Proteins ◽  
Structural Changes ◽  
Ribosomal Subunit ◽  
Compact Structure ◽  
E Coli ◽  
Freeze Dried ◽  
16S Rna ◽  
30S Subunit

The aim of this study is to understand the mechanism of 16S rRNA folding into the compact structure of the small 30S subunit of E. coli ribosome. The assembly of the 30S E. coli ribosomal subunit is a sequence of specific interactions of 16S rRNA with 21 ribosomal proteins (S1-S21). Using dedicated high resolution STEM we have monitored structural changes induced in 16S rRNA by the proteins S4, S8, S15 and S20 which are involved in the initial steps of 30S subunit assembly. S4 is the first protein to bind directly and stoichiometrically to 16S rRNA. Direct binding also occurs individually between 16S RNA and S8 and S15. However, binding of S20 requires the presence of S4 and S8. The RNA-protein complexes are prepared by the standard reconstitution procedure, dialyzed against 60 mM KCl, 2 mM Mg(OAc)2, 10 mM-Hepes-KOH pH 7.5 (Buffer A), freeze-dried and observed unstained in dark field at -160°.

Imaging of ribosomal RNA-protein interactions by dark-field STEM

1989 ◽  
Vol 47 ◽  
pp. 250-251
Author(s):  
M. Boublik ◽  
V. Mandiyan ◽  
S. Tumminia ◽  
J.F. Hainfeld ◽  
J.S. Wall
Keyword(s):  
Nucleic Acid ◽  
16S Rrna ◽  
Dark Field ◽  
Radius Of Gyration ◽  
Central Domain ◽  
The Core ◽  
16S Rna ◽  
30S Subunit ◽  
Core Particles

Success in protein-free deposition of native nucleic acid molecules from solutions of selected ionic conditions prompted attempts for high resolution imaging of nucleic acid interactions with proteins, not attainable by conventional EM. Since the nucleic acid molecules can be visualized in the dark-field STEM mode without contrasting by heavy atoms, the established linearity between scattering cross-section and molecular weight can be applied to the determination of their molecular mass (M) linear density (M/L), mass distribution and radius of gyration (RG). Determination of these parameters promotes electron microscopic imaging of biological macromolecules by STEM to a quantitative analytical level. This technique is applied to study the mechanism of 16S rRNA folding during the assembly process of the 30S ribosomal subunit of E. coli. The sequential addition of protein S4 which binds to the 5'end of the 16S rRNA and S8 and S15 which bind to the central domain of the molecule leads to a corresponding increase of mass and increased coiling of the 16S rRNA in the core particles. This increased compactness is evident from the decrease in RG values from 114Å to 91Å (in “ribosomal” buffer consisting of 10 mM Hepes pH 7.6, 60 mM KCl, 2 m Mg(OAc)2, 1 mM DTT). The binding of S20, S17 and S7 which interact with the 5'domain, the central domain and the 3'domain, respectively, continues the trend of mass increase. However, the RG values of the core particles exhibit a reverse trend, an increase to 108Å. In addition, the binding of S7 leads to the formation of a globular mass cluster with a diameter of about 115Å and a mass of ∽300 kDa. The rest of the mass, about 330 kDa, remains loosely coiled giving the particle a “medusa-like” appearance. These results provide direct evidence that 16S RNA undergoes significant structural reorganization during the 30S subunit assembly and show that its interactions with the six primary binding proteins are not sufficient for 16S rRNA coiling into particles resembling the native 30S subunit, contrary to what has been reported in the literature.

Sign in / Sign up

Export Citation Format

Share Document