The method of investigation employed by the author is as follows:— The heart with the vagus nerve intact having been removed from the body together with a portion of the oesophagus, a thread is tied to the very apex of the ventricle and another to the loose flap which is disclosed at the junction of the two auricles when the two aortic trunks are cut away. The piece of the oesophagus removed with the heart is held firmly in a suitable holder and the heart suspended between two horizontal levers by means of the two threads which are attached to the auricles and ventricle. Between the two levers a clamp is placed, the edges of which can be approximated to any degree by means of a fine micrometer screw j the two limbs of this clamp are placed one on each side of the suspended heart, and by means of the micrometer screw, the tissue between the two edges can be simply held firm or compressed to any extent required. In this way, with the clamp in the auriculo-ventricular groove, the beats of both auricles and ventricle are registered simultaneously and separately; the contractions of the auricles pull the upper lever downwards, those of the ventricle the lower lever upwards. Similarly by varying the position of the clamp the contractions of any two adjacent portions of the heart can be studied, as for example, sinus and 'auricles, base and apex of the ventricle, &c.; heat, cold, and poisons can be applied to the tissue on the one side of the clamp and not on the other; and under all these various conditions the effects of stimulation of the vagus can be observed.