scholarly journals PLASMA VITAMIN C LEVELS IN DERMATOPHILOSIS INFECTED AND ON-INFECTED CATTLE

2021 ◽  
Vol 15 ◽  
pp. 135-138
Author(s):  
S. I. ETTE ◽  
O. B. WILSON ◽  
S. R. AMAKIR1
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Plasma Vitamin ◽  
Infected Cattle ◽  
Blood Samples ◽  
Lower Lever

In two separate experiments, blood samples from dermatophilosis infected and non-infected cattle were analyzed for their vitamin C content. In the first experiment using abattoir samples, streptothricosis infected animals showed significantly lower lever (P < 0.01) Of ascorbic acid in mg per 100 ml of plasma (0.56) than non-infected ones (1.11). In the second experiment using samples from live animals, a non- significant decrease in the concentration of Vitamin in C was also observed in infected (0.94) compared with non-infected animals (1.05)

scholarly journals Role of Vitamin C (Ascorbic Acid) on Human Health- A Review

2005 ◽  
Vol 5 (8) ◽  
pp. 01-12
Author(s):  
Mary K Walingo ◽  
Keyword(s):  
Blood Pressure ◽  
Ascorbic Acid ◽  
Vitamin C ◽  
Human Health ◽  
The Body ◽  
Water Soluble ◽  
Plasma Vitamin ◽  
Risk Of Death ◽  
Increased Risk

Vitamin C, also known as ascorbic acid, abounds in nature and is highly labile. It is a water-soluble vitamin that is lost in large amounts during food processing. It is a vitamin whose prescribed requirement across cultures is not uniform. For example , the prescribed requirement of vitamin C in Great Britain is 30mg/day, while in the U.S.A., it is 60mg/day and 100mg/day in Japan. These variations are unusual and point to the need for further research to establish the acceptable RDAs for diverse populations. The RDA for vitamin C should be more than the amount needed to prevent the occurrence of disease. Vitamin C plays significant functions in the body that enhance its role in the health status of the human body. The biochemical functions of vitamin C include: stimulation of certain enzymes, collagen biosynthesis, hormonal activation, antioxidant, detoxification of histamine, phagocytic functions of leukocytes, formation of nitrosamine, and proline hydroxylation amongst others. These functions are related to the health effects of vitamin C status in an individual. In human health, vitamin C has been associated with reduction of incidence of cancer, blood pressure, immunity, and drug metabolism and urinary hydroxyproline excretion, tissue regeneration. This vitamin is needed for the proper metabolism of drugs in the body through adequate hepatic mixed function oxidase system. Epidemiological data have revealed the preventive and curative role of vitamin C on certain disease conditions in the body though controversies still persist. Vitamin C is effective in protecting against oxidative damage in tissues and also suppresses formation of carcinogens like nitrosamines. There is an inverse relationship with blood pressure and both plasma vitamin C and Vitamin C. Vitamin C has a lowering effect on blood pressure, especially on systolic pressure more than a diastolic pressure. Low levels of plasma vitamin C are associated with stroke and with an increased risk of all cause mortality. Increased consumption of ascorbic acid raises serum ascorbic levels and could decrease the risk of death.

scholarly journals Vitamin E Supplementation and Markers of Oxidative Stress in Indian Acute Myocardial Infarction Patients

2013 ◽  
Vol 5 (2) ◽  
pp. 46-53 ◽  
Author(s):  
Ijen Bhattacharya ◽  
Rahul Saxena ◽  
Raj Saxena ◽  
Alok Milton Lal
Keyword(s):  
Oxidative Stress ◽  
Myocardial Infarction ◽  
Uric Acid ◽  
Vitamin E ◽  
Vitamin C ◽  
Plasma Vitamin ◽  
Blood Samples ◽  
Markers Of Oxidative Stress ◽  
Vitamin E Supplementation

Background: Reactive oxygen species have been identified as mediators of cell injury in a variety of cardiovascular complications including Myocardial Infarction (MI). It is conceivable that vitamin E supplementation can be used therapeutically due to its role in ameliorating antioxidant status and free radicals scavenging activity. Aim: Therefore, the present study was undertaken to assess the markers of oxidative stress i.e. erythrocyte glutathione peroxidase (GSHPx) & malondialdehyde (MDA); plasma vitamin C, E, A and uric acid level in the blood samples of MI patients and to investigate the effect of in-vitro vitamin E supplementation in ameliorating the levels of these antioxidants in the blood sample of MI patients. Material & Method: 60 MI subjects (age group 30-60 years) were taken for the study and 60 healthy individuals served as controls. In-vitro vitamin E supplementation in the blood samples of MI subjects were performed and above mentioned parameters were estimated by using standard methods. Data was compared statistically by using student t-test. Result: Vitamin E supplementation brought about an improved antioxidants status with significantly raised vitamin C, E, A and GSHPx levels (p<0.05, p<0.001), and simultaneously depleted level of erythrocyte MDA (p<0.001) in blood samples of MI subjects. However, plasma uric acid levels remain unaltered (p<0.1). Conclusion: These findings further support the preventive and cardio protective role of vitamin E supplementation in reducing oxidative stress levels in the blood samples of MI patients. DOI: http://dx.doi.org/10.3126/ajms.v5i2.8430 Asian Journal of Medical Science, Volume-5(2) 2014: 46-53

SUPPLEMENTAL VALUE OF ASCORBIC ACID DURING LATE GESTATION ON PIGLET SURVIVAL AND EARLY GROWTH

1983 ◽  
Vol 63 (3) ◽  
pp. 683-687 ◽  
Author(s):  
E. R. CHAVEZ
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Late Gestation ◽  
Dietary Vitamin ◽  
Control Group ◽  
Plasma Vitamin ◽  
Significant Difference ◽  
Vitamin C Supplementation ◽  
Dietary Treatments ◽  
Piglet Survival

Twenty-six pregnant sows were included in four different dietary treatments as follows: (1) Control group with no vitamin C supplementation; (2) vitamin-C-supplemented group receiving 1 g/day ascorbic acid provided in a gelatin capsule; or (3) vitamin-C-supplemented group receiving 1 g/day ascorbic acid in a powder form spread on the top of the pelleted meal; (4) as (3) but receiving 10 g/day ascorbic acid. Vitamin-C-supplemented sows in treatments 2 and 4 showed a significant (P < 0.05) increase in blood plasma vitamin C prefarrowing. Pregnant sows receiving 10 g/day vitamin C supplement showed a significantly (P < 0.05) higher concentration of this vitamin in the urine than either control or sows receiving 1 g/day vitamin C supplementation. Sows in treatment 4 showed a significantly higher (P < 0.05) vitamin C content in colostrum than control animals. During lactation, no significant difference in vitamin C content in milk was observed between control and animals receiving 10 g/day vitamin C supplement. The vitamin C content in the plasma of the piglets at birth indicated that supplementing vitamin C to the mothers, significantly (P < 0.05) increased the concentration of this vitamin in the newborn piglet compared to control animals. However, the piglet survival and growth up to weaning at 3 wk of age was not significantly affected by dietary vitamin C supplementation of the mothers' diet. Key words: Ascorbic acid, gestation, piglet survival, Vitamin C

scholarly journals P21 Scurvy during home parenteral nutrition

2020 ◽  
Vol 105 (9) ◽  
pp. e17.1-e17
Author(s):  
Natalia Iglesias ◽  
Elena Cernat ◽  
Jennifer Trippett
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Parenteral Nutrition ◽  
Reference Range ◽  
Significant Risk ◽  
Home Parenteral Nutrition ◽  
The Other ◽  
Plasma Vitamin ◽  
List Type ◽  
Vitamin Deficiencies

IntroductionDegradation of ascorbic acid due to oxygen presence in parenteral nutrition (PN) is well documented.1 Although patients on home parenteral nutrition (HPN) are routinely monitored for some vitamin deficiencies, plasma vitamin C is rarely measured in this population.We report a case of clinical vitamin C deficiency in a patient with severe dysmotility for whom the only source of nutrition was parenteral nutrition with continuous infusion over 24 hours.MethodsA 6 years old girl with severe gastrointestinal dysmotility following a fundoplication tolerated no enteral feed and could not have time off PN due to hypoglycaemic episodes. She presented with gingival bleeding and epistaxis and also complaining of pain on her arms and shoulders. A clotting was requested which showed prolonged INR and she was treated with IV vitamin K. An x-ray of her wrist and shoulder showed osteopenia but no other abnormalities. Vitamin C measurement was requested.ResultsPlasma vitamin C was low at 3.5 micromol/L (26.1–84.6) which confirmed the diagnosis of scurvy. She was treated with 3 doses of Pabrinex® over 3 days (providing total of 450 mg vitamin C). 100 mg of ascorbic acid were also added to her PN, providing double the baseline amount. Plasma vitamin C measured after two weeks had risen to normal at 45 micromol/L. Her bleeding and pains resolved over a few days. During the next year plasma vitamin C was measured every three months and remained within reference range. She remained clinically well with no recurrence of bleeding. Due to methodology limitations, the amount of vitamin C in the PN bag could not be tested. Therefore we decided to measure plasma vitamin C in two other patients that had PN as the only source of nutrition and given over 12 hours. In both cases the result was within the reference range at 58.7 and 38 micromol/L respectively. Published literature2 suggests that temperature contributes to vitamin C degradation. The PN fluid would have been at room temperature for around 24 hours for the patient that developed scurvy, compared to half this time in the other two.ConclusionsThis case highlights that there is a significant risk of vitamin C degradation due to the oxygen present within the PN bag. However, the fact that for the other 2 patient’s plasma vitamin C was normal suggests that presence of oxygen alone might not be enough to cause vitamin C insufficiency. Temperature might be a contributing factor to vitamin C degradation, as has been shown with enteral feed.3 From these cases we concluded that measurement of vitamin C during HPN would be indicated not only if there were suggestive symptoms, but should also be added in to routine monitoring in any patient with a 24 hour infusion time and extremely restricted enteral intake.ReferencesNHS Pharmaceutical Quality Assurance Committee, Standard protocol for derivation and assessment of stability Part 4 – Parenteral Nutrition, May 2016Turmezei J, Javorszky E, Szabo E, et al. Effect of storage temperature on stability of total parenteral nutrition admixtures prepared for infants. Acta Poloniae Pharmaceutica, Drug Research 2015:72:843–9Gorman SR, Armstrong G, Allen KR, et al. Scarcity in the midst of plenty: enteral tube feeding complicated by scurvy. Journal of Pediatric Gastroenterology and Nutrition 2002;35:93–95

scholarly journals Bioavailability of vitamin C from kiwifruit in non-smoking males: determination of ‘healthy’ and ‘optimal’ intakes

2012 ◽  
Vol 1 ◽  
Author(s):  
Anitra C. Carr ◽  
Juliet M. Pullar ◽  
Stephanie Moran ◽  
Margreet C. M. Vissers
Keyword(s):  
Vitamin C ◽  
Washout Period ◽  
Plasma Levels ◽  
Plasma Vitamin ◽  
Actinidia Chinensis ◽  
Blood Samples ◽  
Study Population ◽  
Essential Nutrient ◽  
Male University Students

AbstractVitamin C is an essential nutrient in humans and must be obtained through the diet. The aim of this study was to determine vitamin C uptake in healthy volunteers after consuming kiwifruit (Actinidia chinensisvar.Hort. 16A), and to determine the amount of fruit required to raise plasma vitamin C to ‘healthy’ (i.e. >50 µmol/l) and ‘optimal’ or saturating levels (i.e. >70 µmol/l). Leucocyte and urinary vitamin C levels were also determined. A total of fifteen male university students with below average levels of plasma vitamin C were selected for the study. Weekly fasting blood samples were obtained for a 4-week lead-in period and following supplementation with, sequentially, half, one, two and three Gold kiwifruit per d for 4–6 weeks each, followed by a final 4-week washout period. The results showed that addition of as little as half a kiwifruit per d resulted in a significant increase in plasma vitamin C. However, one kiwifruit per d was required to reach what is considered healthy levels. Increasing the dose of kiwifruit to two per d resulted in further increases in plasma vitamin C levels as well as increased urinary output of the vitamin, indicating that plasma levels were saturating at this dosage. Dividing the participants into high and low vitamin C groups based on their baseline plasma and leucocyte vitamin C levels demonstrated that it is critical to obtain a study population with low initial levels of the vitamin in order to ascertain a consistent effect of supplementation.

scholarly journals Association between a SLC23A2 Gene Variation, Plasma Vitamin C Levels, and Risk of Different Diseases

2021 ◽  
pp. 6-14
Author(s):  
Hassan Sadek Darwish ◽  
Amal Al-Ghammari ◽  
Fatma Al-Amrani ◽  
Khaloud Al-Saadi ◽  
Munira Al-Hatmi ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Lipid Membranes ◽  
Protein Product ◽  
The Body ◽  
Water Soluble ◽  
Plasma Vitamin ◽  
Accession Number ◽  
Coronary Syndrome ◽  
Increased Risk

Background: Vitamin C is an important plasma water-soluble antioxidant that plays an essential role in the absorption of iron, detoxification of exogenous compounds, and remaking vitamin E for the protection of lipid membranes. In addition, vitamin C is essential in the synthesis of collagen. Vitamin C concentrations of plasma are determined by dietary intake and genetic factors. Ascorbic acid is the functional form of vitamin C, which is transported into the cell through sodium vitamin C transporters (SVCTs). There are two forms of SVCTs which are SVCT1 encoded by the SLC23A1 gene and SVCT2 encoded by the SLC23A2. The SLC23A2 gene locus on human chromosome 20P12. It expresses in most human tissues, except lung and skeletal muscle that it is important in regulating the intracellular concentration of ascorbic acid to protect the cell from oxidative stress and promote type 1 collagen maturation. Maintaining proper concentrations of plasma and cellular vitamin C concentration is important for the normal metabolic function of the body and preventing several diseases. In the contrast, a low concentration of vitamin C caused by SLC23A2 variation can cause several chronic diseases. Our systematic review discusses four diseases related to the variation of SLC23A2 gene and plasma vitamin C levels which are glaucoma, acute coronary syndrome among women, gastric cancer, and HPV16-associated head and neck cancer. Methods: By using NCBI databases, specifically GenBank to analyze DNA sequence and mRNA sequence of SLC23A2 gene. GenBank file format was helpful to extract an accession number of the gene, number of amino acids, number of exons and introns, and length of nucleotides. FASTA format was also useful to retrieve the nucleotide sequence and get the function of the protein. BLAST was used to compare the protein product of the SLC23A2 gene between humans and Macaca mulatta (Rhesus monkey). Results: the accession number of the SLC23A2 gene was NC_000020.11, the number of exons found was 18, and the gene was located in chromosome 20. This gene encodes one of the two required transporters, and the encoded protein accounts for tissue-specific uptake of vitamin C. This gene had an official symbol of SLC23A1. And they found a significant association between the single-nucleotide polymorphism (SNP) rs1279683 (A > G) in SLC23A2 and an increased risk of POAG in homozygous G allele (GG) carriers. Also, POAG patients with this SNP appear to have a significantly lower level of plasma vitamin C compared to other genotypes. Finally, many organisms have the same gene, such as dogs, mice, rats, and chickens. Conclusion: there is a significant association between SLC23A2 gene mutation, increased risk for vitamin C deficiency, and several diseases. SNP in the SLC23A2 gene was significantly associated with a higher risk of POAG in GG allele carriers as well as lower plasma vitamin C concentration.

Stability of antioxidant vitamins in whole human blood during overnight storage at 4°C and frozen storage up to 6 months

2018 ◽  
Vol 88 (3-4) ◽  
pp. 151-157 ◽  
Author(s):  
Scott W. Leonard ◽  
Gerd Bobe ◽  
Maret G. Traber
Keyword(s):  
Ascorbic Acid ◽  
Whole Blood ◽  
Healthy Subjects ◽  
Frozen Storage ◽  
Blood Collection ◽  
Plasma Samples ◽  
Optimal Conditions ◽  
Plasma Ascorbic Acid ◽  
Blood Samples ◽  
Sample Handling

Abstract. To determine optimal conditions for blood collection during clinical trials, where sample handling logistics might preclude prompt separation of erythrocytes from plasma, healthy subjects (n=8, 6 M/2F) were recruited and non-fasting blood samples were collected into tubes containing different anticoagulants (ethylenediaminetetra-acetic acid (EDTA), Li-heparin or Na-heparin). We hypothesized that heparin, but not EDTA, would effectively protect plasma tocopherols, ascorbic acid, and vitamin E catabolites (α- and γ-CEHC) from oxidative damage. To test this hypothesis, one set of tubes was processed immediately and plasma samples were stored at −80°C, while the other set was stored at 4°C and processed the following morning (~30 hours) and analyzed, or the samples were analyzed after 6 months of storage. Plasma ascorbic acid, as measured using HPLC with electrochemical detection (LC-ECD) decreased by 75% with overnight storage using EDTA as an anticoagulant, but was unchanged when heparin was used. Neither time prior to processing, nor anticoagulant, had any significant effects upon plasma α- or γ-tocopherols or α- or γ-CEHC concentrations. α- and γ-tocopherol concentrations remained unchanged after 6 months of storage at −80°C, when measured using either LC-ECD or LC/mass spectrometry. Thus, refrigeration of whole blood at 4°C overnight does not change plasma α- or γ-tocopherol concentrations or their catabolites. Ascorbic acid is unstable in whole blood when EDTA is used as an anticoagulant, but when whole blood is collected with heparin, it can be stored overnight and subsequently processed.

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