CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage

The Ff family of filamentous bacteriophages infect gram-negative bacteria, but do not cause lysis of their host cell. Instead, new virions are extruded via the phage-encoded pIV protein, which has homology with bacterial secretins. Here, we determine the structure of pIV from the f1 filamentous bacteriophage at 2.7 Å resolution by cryo-electron microscopy, the first near-atomic structure of a phage secretin. Fifteen f1 pIV subunits assemble to form a gated channel in the bacterial outer membrane, with associated soluble domains projecting into the periplasm. We model channel opening and propose a mechanism for phage egress. By single-cell microfluidics experiments, we demonstrate the potential for secretins such as pIV to be used as adjuvants to increase the uptake and efficacy of antibiotics in bacteria. Finally, we compare the f1 pIV structure to its homologues to reveal similarities and differences between phage and bacterial secretins.

Numerical simulation of the shock-wave structure of a reacting hydrogen-air mixture in a model channel

The paper deals with the results of numerical simulation of hydrogen-air combustion in a supersonic flow of a model channel of a known configuration, investigated in the “HyShot” project. The simulation is carried out by solving the Favre-averaged system of Navier-Stokes equations, supplemented by a turbulence and combustion model and a chemical-kinetic mechanism. The influence of different throat heights on the performance of the model due to combustion efficiency and total pressure loss coefficients is investigated.

Parametric computational study of particle trajectory deviation probabilistic nature influencing the unevenness of their localization in the model tract

The purpose of this research was to investigate the spatial structure of a two-phase flow in a supersonic model channel of circular cross-section with a diameter of the cylindrical part of ~10 mm. For modeling, we used the Euler-Lagrange approach in combination with a probabilistic estimate of the dispersed particles deviation from their base trajectory. Chromium-nickel alloy particles with a diameter of 15 to 40 μm move in the channel in a special way, which was considered in the paper. Furthermore, we analyzed how the nature of the distribution function of the particle’s root-mean-square deviation from its base trajectory influences the quality of mixing of the dispersed phase with the flow and the number of particles interacting with the walls of the flow path.

CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage

The Ff family of filamentous bacteriophages infect gram-negative bacteria, but do not cause lysis of their host cell. Instead, new virions are extruded via the phage-encoded pIV protein, which has homology with bacterial secretins. Here, we have determined the structure of pIV from the f1 filamentous bacteriophage at 2.7 A resolution by cryo-electron microscopy, the first near-atomic structure of a phage secretin. Fifteen f1 pIV monomers assemble to form a gated channel in the bacterial outer membrane, with associated soluble domains projecting into the periplasm. We model channel opening and propose a mechanism for phage-mediated gate movement. By single-cell microfluidics experiments, we demonstrate the potential for secretins such as pIV to be used as adjuvants to increase the uptake and efficacy of antibiotics in bacteria. Finally, we compare the f1 pIV structure to its homologues to reveal similarities and differences between phage and bacterial secretins.

A Simulational Performance of 5G MIMO Systems applying UFMC( Universal Filtered Multicarrier) Study

The advanced wireless communication system uses the new technology, preparing the limited radio spectrum and its low expensive resource. The Scenario of Wireless communication is needed for the flexible mechanism with available time and frequency resources. The OFDM system, which is a multicarrier process with a remote framework in Long Term Evolution (LTE), 4G, and 5G. Using of UFMC(Universal_Filtered_Multicarrier)system with OFDM system symmetrical recurrence division multiply with modulation system and the correlation between them. Using of UFMC system with QAM into the MIMO system the result of subchannel, the model channel correlation recreated in “ A Simulational Performance of 5G MIMO Systems applying UFMC (Universal_Filtered_Multicarrier ) Study” result shown in MATLAB.

A performance enhancement of physical layer at Wi-MAX system with RLDPC code

<span>In present wireless communication network, the error correction codes plays the major role for efficient data transmission in noisy environments. To get minimum BER and PAPR has been the main aim towards the field in forward error control coding. Majority of the researchers has considered turbo codes at specific SNR over AWGN channel but have complexity issues with the iterative output decoder and causes degradation in the Wi-Max network system. In this paper, the author presents and evaluates WiMAX physical layer performance with using MIMO technologies, where a Robust-LDPC technique of coding and decoding in OFDM based WiMAX system is consider. The decoding method of RLDPC has processed by Belief Propagation at the logarithmic domain in an iterative manner, the proposed methodology shows good decoding outcome for RLDPC codes at Rician and Rayleigh channel. Moreover, the applicability of our proposed model channel codes is defined under IEEE Wi-MAX standard and the results analysis is done under different code-rate and modulation schemes.</span>

Uncertainty‐driven business cycles: Assessing the markup channel

Precautionary pricing and increasing markups in representative‐agent DSGE models with nominal rigidities are commonly used to generate negative output effects of uncertainty shocks. We assess whether this theoretical model channel is consistent with the data. Three things stand out. First, consistent with precautionary wage setting, we find that wage markups increase after uncertainty shocks. Second, the impulse responses of price markups are largely inconsistent with the standard model, both at the aggregate as well as the industry level. Finally, and in contrast to times‐series evidence, our theoretical model robustly predicts that uncertainty shocks have a quantitatively small impact on the economy.

Targeted Esterase induced Dye loading supports Calcium Imaging in Eukaryotic Cell-Free Systems

ABSTRACTCalcium imaging is an important functional tool for addressing ion channels, transporters and pumps for drug screening in living cells. Depicted eukaryotic cell-free systems utilize microsomes, derived from endoplasmic reticulum to incorporate the synthesized membrane proteins. Absence or inadequate amount of carboxylesterase in the endoplasmic reticulum of eukaryotic cells, which is necessary to cleave the acetoxymethyl ester moiety of the chemical calcium indicators, advocates the hindrance to perform calcium imaging in microsomes. In this work, we try to overcome this drawback and adapt the cell-based calcium imaging principle to a cell-free protein synthesis platform. Carboxylesterase synthesized in a Spodoptera frugiperda Sf21 lysate translation system is established as a viable calcium imaging tool and hTRPV1 is used as a model channel protein to demonstrate the realization of this concept.