Antibiotic treatment of the breadcrumb sponge Halichondria panicea and subsequent recolonization v1

This protocol generates sponges (Halichondria panicea) with a disturbed microbiome under controlled experimental conditions, in order to study bacterial recolonization dynamics. Bacteria-bacteria interactions can be analysed with this set-up within the host environment aiming at a better understanding of sponge-microbe symbiosis in vivo. It is divided into the sections 1) preparation, 2) antibiotic treatment and recovery phase, 3) recolonization with the natural microbiome and 4) sampling.

DNA/RNA extraction and qPCR protocol to assess bacterial abundance in the spongeHalichondria panicea v1

This protocol summarizes experience and recommendations regarding DNA and RNA extractions from the sponge Halichondria panicea and qPCR to quantify bacterial abundance. We have used both bacterial and sponge DNA and RNA for subsequent qPCR. Further, bacterial 16S rRNA amplicon sequencing was performed on DNA and sponge transcriptomes were sequenced successfully from extracted RNA.

SECONDARY METABOLITES PRODUCED BY MARINE ACTINOMYCETE STREPTOMYCES SP. G246

In a recent study, we described two new lavandulylated flavonoids, along with eight known compounds from the culture broth of a Streptomyces sp. (strain G246), isolated from the sponge Halichondria panicea, collected in the sea of Son Tra peninsula (Da Nang). A comparison study was conducted to differentiate between solid and liquid fermentation technique for secondary metabolites production of strain G246. In this paper, we report the isolation and structural characterization of 9 secondary metabolites (1-9) from strain G246 by solid state fermentation. Compound 3 was the only one similarity between these fermentation techniques.