Metabolomic Profiles of the Creeping Wood Sorrel Oxalis corniculata in Radioactively Contaminated Fields in Fukushima: Dose-Dependent Changes in Key Metabolites

The biological impacts of the Fukushima nuclear accident, in 2011, on wildlife have been studied in many organisms, including the pale grass blue butterfly and its host plant, the creeping wood sorrel Oxalis corniculata. Here, we performed an LC–MS-based metabolomic analysis on leaves of this plant collected in 2018 from radioactively contaminated and control localities in Fukushima, Miyagi, and Niigata prefectures, Japan. Using 7967 peaks detected by LC–MS analysis, clustering analyses showed that nine Fukushima samples and one Miyagi sample were clustered together, irrespective of radiation dose, while two Fukushima (Iitate) and two Niigata samples were not in this cluster. However, 93 peaks were significantly different (FDR < 0.05) among the three dose-dependent groups based on background, low, and high radiation dose rates. Among them, seven upregulated and 15 downregulated peaks had single annotations, and their peak intensity values were positively and negatively correlated with ground radiation dose rates, respectively. Upregulated peaks were annotated as kudinoside D (saponin), andrachcinidine (alkaloid), pyridoxal phosphate (stress-related activated vitamin B6), and four microbe-related bioactive compounds, including antibiotics. Additionally, two peaks were singularly annotated and significantly upregulated (K1R1H1; peptide) or downregulated (DHAP(10:0); decanoyl dihydroxyacetone phosphate) most at the low dose rates. Therefore, this plant likely responded to radioactive pollution in Fukushima by upregulating and downregulating key metabolites. Furthermore, plant-associated endophytic microbes may also have responded to pollution, suggesting their contributions to the stress response of the plant.

A Systematic Review on the Toxicological Implications of Microplastic to Human Health

The environmental, economic, and social impacts of micro and nano plastic (MNPs) pollution have caused significant global concern, which is exacerbated by the MNPs associated chemicals and biological entities that could directly or indirectly impact human health. The systematic review of research accomplished on this issue could enhance the understanding surrounding the toxicological risks of MP and its pathways to the human system. A significant amount of research is available on microplastic; still, the research executed to define the chemical impact and risk scenarios of microplastics on human health is limited. The objective of this study was to catalogue the literature on the toxicological impacts of MNPs on humans and the resulting biological consequences. A total of 95 publications were reviewed following the Cochrane protocol and screened based on the direct and indirect health impacts of MNPs to the human systems using the RevMan 5 software. Processing of collected data resulted in a total of 19 publications (until July 2021) that fulfilled the inclusion criteria and provided evidence of the potential impacts of MNPs, particularly in relation to the average sizes of Large MPs - 5mm - 1mm; MPs - 1mm - 0.1μm; NPs - 1-1000nm. Detailed analysis of data categorized into scenarios of chemical, toxicological, and biological impacts of MNPs on human health indicated that additives and associated contaminants of MNPs would pose a significant risk to human health. This is the first review that streamlines the notion that indirect impacts of MNPs caused due to MNP-associated chemicals are more severe than the direct impacts such as ingestion of MNPs.

An overview of bottom trawl selectivity in the Mediterranean Sea

In the Mediterranean Sea, where bottom trawling for demersal species is the most important fishery in terms of landings, around 75% of the assessed fish stocks are overfished. Its status as one of the world’s most heavily exploited seas and the one subject to the highest trawling pressure has become a global concern. An extensive review of bottom trawl selectivity studies was performed to assess the sustainability of this fishery in the Mediterranean; the selectivity parameters were collected from 93 peer-reviewed publications of 10 countries, totalling 742 records and 65 species. The review highlighted that i) the catch of bottom trawls commonly employed in the Mediterranean, even complying with current regulations on codend meshes, still includes immature individuals for 64-68% of the species investigated, and individuals under the minimum conservation reference size (MCRS) for 78% of the species investigated, and that ii) the MCRS set for 59% of the species analyzed is well below their length at first maturity, and is therefore ecologically inadequate. Although square-mesh codends are slightly more selective, the models developed herein demonstrate that improving size and species selectivity would require considerably larger meshes, which may significantly reduce profitability. The urgent need to reduce the biological impacts of bottom trawling in the Mediterranean should be addressed by promoting the adoption of more ecologically sustainable fishing gears through the introduction of more selective meshes or gear modifications.

Impact of ABC Transporters in Osteosarcoma and Ewing’s Sarcoma: Which Are Involved in Chemoresistance and Which Are Not?

The ATP-binding cassette (ABC) transporter superfamily consists of several proteins with a wide repertoire of functions. Under physiological conditions, ABC transporters are involved in cellular trafficking of hormones, lipids, ions, xenobiotics, and several other molecules, including a broad spectrum of chemical substrates and chemotherapeutic drugs. In cancers, ABC transporters have been intensely studied over the past decades, mostly for their involvement in the multidrug resistance (MDR) phenotype. This review provides an overview of ABC transporters, both related and unrelated to MDR, which have been studied in osteosarcoma and Ewing’s sarcoma. Since different backbone drugs used in first-line or rescue chemotherapy for these two rare bone sarcomas are substrates of ABC transporters, this review particularly focused on studies that have provided findings that have been either translated to clinical practice or have indicated new candidate therapeutic targets; however, findings obtained from ABC transporters that were not directly involved in drug resistance were also discussed, in order to provide a more complete overview of the biological impacts of these molecules in osteosarcoma and Ewing’s sarcoma. Finally, therapeutic strategies and agents aimed to circumvent ABC-mediated chemoresistance were discussed to provide future perspectives about possible treatment improvements of these neoplasms.

Parasitism-evoked horizontal gene transfer between plants as a novel trigger for specialized metabolism evolution

Recent genomic studies of parasitic plants have revealed that there are numerous footprints indicative of horizontal gene transfer (HGT) to the parasites from their host plants. However, the molecular mechanisms and biological impacts of this phenomenon have remained largely unknown. Here, we made the striking observation that two parasitic dodders, Cuscuta campestris and C. australis, have functional homologues of Si_CYP81Q1, which encodes piperitol/sesamin synthase (PSS) in the phylogenetically remote plant Sesamum indicum (sesame). The apparent lack of sequence similarity between the regions flanking PSS in Sesamum and Cuscuta spp. suggests the occurrence of HGT tightly associated with the PSS gene. Upon parasitism, C. campestris induced expression of the host Si_CYP81Q1 at the parasitic interface and mature and intron-retained Si_CYP81Q1 mRNA was transferred to C. campestris, suggesting that CYP81Q1 was translocated via RNA-mediated HGT. Thus, parasitism-evoked HGT might have had an unexpected role in the metabolic evolution of plants.

Cold Atmospheric Plasma Cancer Treatment, a Critical Review

Cold atmospheric plasma (CAP) is an ionized gas, the product of a non-equilibrium discharge at atmospheric conditions. Both chemical and physical factors in CAP have been demonstrated to have unique biological impacts in cancer treatment. From a chemical-based perspective, the anti-cancer efficacy is determined by the cellular sensitivity to reactive species. CAP may also be used as a powerful anti-cancer modality based on its physical factors, mainly EM emission. Here, we delve into three CAP cancer treatment approaches, chemically based direct/indirect treatment and physical-based treatment by discussing their basic principles, features, advantages, and drawbacks. This review does not focus on the molecular mechanisms, which have been widely introduced in previous reviews. Based on these approaches and novel adaptive plasma concepts, we discuss the potential clinical application of CAP cancer treatment using a critical evaluation and forward-looking perspectives.