scholarly journals Metagenomic Analysis of Microbial Communities From Acidic Marshland Habitat of India

2021 ◽  
Author(s):  
Khairun Nisha ◽  
Jithin S Sunny ◽  
Anuradha Natrajan ◽  
Lilly M. Saleena
Keyword(s):  
Functional Analysis ◽  
Microbial Communities ◽  
Aquatic Ecosystems ◽  
High Throughput Sequencing ◽  
Degradation Pathway ◽  
Taxonomic Analysis ◽  
Benzoate Degradation ◽  
Polycyclic Aromatic ◽  
Xenobiotic Biodegradation ◽  
Polycyclic Aromatic Hydrocarbon Degradation

Abstract Understanding the diversity and functioning of microbial communities in acidic marsh land is extremely less investigated in contrast to soils and aquatic ecosystems. This study implemented Illumina high-throughput sequencing to explore the microbial communities and xenobiotic degrading enzymes in the acidic marshland. Taxonomic analysis using SILVA SSU database stated that Proteobacteria (66.74 %), Bacteroidetes (6.98%) and acidobacteria (2.35 %) were the most abundant phylum in the acidic marshland. Functional analysis by SEED subsystems showed that 1.62 % substitute to metabolism of aromatic compound while KO indicates 1.86% of metabolism are involved in xenobiotic biodegradation. KO analysis also indicated the benzoate degradation pathway (ko00362) are predominant while Chlorocyclohexane and chlorobenzene degradation pathway (ko00361), Polycyclic aromatic hydrocarbon degradation pathway (ko00624) Aminobenzoate degradation pathway (ko00627) is largely present in the acidic marshland.

scholarly journals Complete Genome Report of Sphingobium Yanoikuyae S72: A Bacterium Capable of Degrading Hydrocarbons

2021 ◽  
Author(s):  
Eric Sanchez-Lopez ◽  
Temidayo Oluyomi Elufisan ◽  
Patricia Bustos ◽  
Claudia Paola Charles-Mendoza ◽  
Alberto Mendoza-Hererra ◽  
...  
Keyword(s):  
Complete Genome ◽  
Minimal Medium ◽  
Core Genome ◽  
De Novo ◽  
Degradation Pathway ◽  
General Function ◽  
Protein Coding ◽  
Benzoate Degradation ◽  
Sorghum Plant ◽  
Polycyclic Aromatic

Abstract Sphingobium yanoikuyae S72 was isolated from the rhizosphere of sorghum plant, Mexico. Its role on the degradation of polycyclic aromatic hydrocarbon (PAH) was evaluated in a minimal medium containing one of biphenyl, naphthalene, phenanthrene, xylene and Toluene as the only carbon source. It grew effectively in each minimal medium (Bushnell Haas, BH). Gas column chromatography–mass spectrometry (GC-MS) analysis on the metabolite recovered after S72’s growth in BH showed that it successfully degraded the PAHs tested resulting in the degradation of biphenyl (85%), phenanthrene (93%), naphthalene (81%), xylene (19%) and toluene (30%). The complete genome of the S72 was sequenced to evaluate the genetic bases of S72`s degradation of PAH. The genome was assembled de novo and reduced to 1 contig and consists of 5,532,623 bp, 5005 protein-coding genes. The analysis of the COG categories in S72 in comparison with other Sphingobium yanoikuyae and other PAH degrading gene, showed that it has in higher abundance in some categories that have been associated with the degradation of PAH (energy production and conversion (COG C, 6.35), carbohydrate transport and metabolism (COG G, 6.20%), lipid transport and metabolism (COG I, 6.99), secondary metabolites biosynthesis (COG Q, 4.52%), general function prediction only in transport and catabolism (COG R, 9.93),) Pan-core genome analysis revealed that about 126 unique genes in S72 are associated with the degradation of xenobiotics and PAHs. Most gene found in S72 are associated with the peripheral degradation pathway for PAH. Based on our observation we proposed a possible benzoate degradation pathway.

scholarly journals Degradation of Polycyclic Aromatic Hydrocarbons by Consortium 5H Under Hypersaline Conditions

2021 ◽  
Author(s):  
Jiaqi Jin ◽  
Zuotao Zhang ◽  
Lu Han ◽  
Chicheng Yan ◽  
Xinxing Ding ◽  
...  
Keyword(s):  
Aromatic Hydrocarbons ◽  
High Throughput Sequencing ◽  
Degradation Pathway ◽  
Hypersaline Environments ◽  
Wide Range ◽  
Significant Difference ◽  
Polycyclic Aromatic ◽  
Alkaline Conditions ◽  
Pahs Concentration ◽  
Good Potential

Abstract PAHs were widely detected accumulated in saline and hypersaline environments. The halotolerant and halophilic microbes were considered as the most suitable player for the elimination of PAHs in such environments. In this study, consortium 5H was enriched under 5% salinity that was able to completely degrade phenanthrene in 5 days. By high-throughput sequencing, consortium 5H was identified mainly composed of Methylophaga, Marinobacter and Thalassospira. Combined with the investigation of intermediates and enzymatic activities, the degradation pathway of consortium 5H on phenanthrene was proposed. Meanwhile, consortium 5H was identified with ability to tolerate a wide range of salinity (1% to 10%) and initial PAHs concentration (50 mg/L to 400 mg/L). It was also able to work under neutral to week alkaline conditions (pH from 6 to 9) and the phytotoxicity of the produced intermediates showed no significant difference with distilled water. This study expanded the knowledge of PAH-degradation under hypersaline environments and consortium 5H was proposed with a good potential for the elimination of PAHs pollution under saline/hypersaline environments.

scholarly journals Extremely Halophilic Biohydrogen Producing Microbial Communities from High-Salinity Soil and Salt Evaporation Pond

Fuels ◽  
2021 ◽  
Vol 2 (2) ◽  
pp. 241-252
Author(s):  
Dyah Asri Handayani Taroepratjeka ◽  
Tsuyoshi Imai ◽  
Prapaipid Chairattanamanokorn ◽  
Alissara Reungsang
Keyword(s):  
Microbial Communities ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
High Salinity ◽  
Amplicon Sequencing ◽  
Spatial Proximity ◽  
Lignocellulosic Waste ◽  
Evaporation Pond ◽  
Operational Taxonomic Units ◽  
Determining Factor

Extreme halophiles offer the advantage to save on the costs of sterilization and water for biohydrogen production from lignocellulosic waste after the pretreatment process with their ability to withstand extreme salt concentrations. This study identifies the dominant hydrogen-producing genera and species among the acclimatized, extremely halotolerant microbial communities taken from two salt-damaged soil locations in Khon Kaen and one location from the salt evaporation pond in Samut Sakhon, Thailand. The microbial communities’ V3–V4 regions of 16srRNA were analyzed using high-throughput amplicon sequencing. A total of 345 operational taxonomic units were obtained and the high-throughput sequencing confirmed that Firmicutes was the dominant phyla of the three communities. Halanaerobium fermentans and Halanaerobacter lacunarum were the dominant hydrogen-producing species of the communities. Spatial proximity was not found to be a determining factor for similarities between these extremely halophilic microbial communities. Through the study of the microbial communities, strategies can be developed to increase biohydrogen molar yield.

Effect of biofilm parameters and extracellular polymeric substance composition on polycyclic aromatic hydrocarbon degradation

RSC Advances ◽  
2016 ◽  
Vol 6 (62) ◽  
pp. 57540-57551 ◽  
Author(s):  
Neelam Mangwani ◽  
Sudhir K. Shukla ◽  
Supriya Kumari ◽  
Surajit Das ◽  
T. Subba Rao
Keyword(s):  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Marine Bacteria ◽  
Extracellular Polymeric Substance ◽  
Significant Variation ◽  
Hydrocarbon Degradation ◽  
Substance Composition ◽  
Biofilm Architecture ◽  
Polycyclic Aromatic ◽  
Polycyclic Aromatic Hydrocarbon Degradation

This study with ten marine isolates demonstrates that the attached phenotypes of the marine bacteria showed significant variation in biofilm architecture and, in turn, biodegradation of PAHs.

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