Aromatic and medicinal plants used in traditional medicine in the region of Tiaret, North West of Algeria

Herbal medicine is gaining an increasing importance in the management of various ailments, but little is known about traditional practices in Algeria. This ethnopharmacological study aims to document medicinal and aromatic plants used in traditional medicine in the region of Tiaret (northwest of Algeria) in order to contribute to safeguard the local pharmacopoeia as cultural heritage, and to provide a scientific basis for developing novel strategies for such practices which can help for drugs discovery. Semi-structured interviews with 64 traditional healers and herbalists were realized in the region of Tiaret (Algeria) throughout field studies achieved from December 2018 to May 2020. Interviews covered sociodemographic information, popular and vernacular names of the medicinal plants, mode of use and toxicity among other data. Results reveal the use of 107 plant species belonging to 45 families and 97 genera for the treatment of various ailments. Lamiaceae, Apiaceae and Asteraceae were the most represented families. The most frequently cited species were Senna alexandrina Mill. (FC=27), Atriplex halimus L. and Bunium incrassatum Amo (FC=23 each), Foeniculum vulgare Mill. (FC=22), and Matricaria chamomilla L. (FC=21). However, the higher use values were reported for Nigella sativa L. (UV=1.5), Trigonella foenum-graecum L. (UV=1.38), Thymus serpyllum L. (UV=1.2), Ziziphus lotus (L.) Lam. (UV=1.14), Urtica dioica L. (1.13), and Senna alexandrina Mill. (UV=0.52) respectively. Interestingly, Bunium incrassatum Amo, Echinops spinosissimus Turra, Cucurbita moschata Duchesne, Pennisetum glaucum (L.) R.Br and Malus domestica Borkh. were reported for the first time as medicinal plants in the north Africa and Algeria. Moreover, 246 new therapeutic uses were described. It should be noted that Pistacia atlantica Desf., Tetraclinis articulata (Vahl) Mast., Oudneya africana R. Br., Euphorbia guyoniana Boiss. & Reut, Teucrium polium L. and Marrubium deserti (Noë) Coss. are endemic to North Africa-Algeria, Northern and Central Sahara. Furthermore, Artemisia herba-alba Asso, Anacyclus pyrethrum (L.) Lag., Cuminum cyminum L., Saussurea costus (Falc.) Lipsch., Boswellia sacra Flueck. and Pistacia atlantica Desf. are considered threatened, rare or endangered species. Our findings are relevant not only for the future studies and experiments in the search for novel compounds, but also for the safeguard of traditional knowledge and biodiversity.

Leaf powder supplementation of Senna alexandrina ameliorates oxidative stress, inflammation, and hepatic steatosis in high-fat diet-fed obese rats

Obesity is an enduring medical issue that has raised concerns around the world. Natural plant extracts have shown therapeutic potential in preventing oxidative stress and inflammation related to obesity complications. In this study, Senna alexandrina Mill. leaves were utilized to treat high-fat diet-related metabolic disorders and non-alcoholic fatty liver diseases. Plasma biochemical assays were conducted to determine the lipid profiles and oxidative stress parameters, and the gene expression of antioxidant enzymes and inflammatory mediators was measured. Histological stained livers of high-fat diet-fed rats were observed. S. alexandrina leaf powder supplementation prevented the increase in cholesterol and triglyceride levels in high-fat diet-fed rats. Moreover, S. alexandrina leaves also reduced lipid peroxidation and nitric oxide production in these rats. Prevention of oxidative stress by S. alexandrina leaf supplementation in high-fat diet-fed rats is regulated by enhancing the antioxidant enzyme activity, followed by the restoration of corresponding gene expressions, such as NRF-2, HO-1, SOD, and CAT. Histological staining provides further evidence that S. alexandrina leaf supplementation prevents inflammatory cell infiltration, lipid droplet deposition, and fibrosis in the liver of high-fat diet-fed rats. Furthermore, this investigation revealed that S. alexandrina leaf supplementation controlled non-alcoholic fatty liver disease by modulating the expression of fat metabolizing enzymes in high-fat diet-fed rats. Therefore, S. alexandrina leaf supplementation inhibits fatty liver inflammation and fibrosis, suggesting its usefulness in treating non-alcoholic steatohepatitis. Thus, this natural leaf extract has potential in treatment of obesity related liver dysfunction.

Efikasi Daun Jati Cina dalam Mengatasi Konstipasi

Konstipasi merupakan gangguan gastrointestinal yang umum terjadi pada seluruh tingkat usia dan gejalanya dapat secara signifikan mempengaruhi kualitas hidup penderita. Dewasa ini, teh maupun kapsul berisi serbuk daun jati cina banyak dipasarkan kepada masyarakat sebagai produk laksatif untuk penanganan konstipasi juga sebagai penurun berat badan. Pada daun jati cina dapat ditemukan beberapa komponen bioaktif yang berperan dalam manfaat-manfaat kesehatannya seperti senyawa antraquinoid (senosida (A, B, C, dan D), fenol, flavonoid, sakarin, turunan naftalen, fitosterol, serta senyawa-senyawa lain. Metode analisis yang digunakan adalah dengan studi literatur dengan cara meringkas topik diskusi lalu membandingkan hasilnya dalam artikel, dengan kriteria inklusinya adalah jurnal-jurnal saintifik nasional dan internasional dalam sepuluh tahun terakhir (2011-2020) yang diakses dari situs-situs terpercaya. Melalui hasil pencarian pada situs NCBI, Elsevier, Pubmed, dan lainnya dengan kata kunci yang digunakan berupa daun jati cina, laxative, alexandrian senna, weight-loss, Senna alexandrina Mill., didapatkan 12.549 artikel namun hanya 30 artikel yang dianggap relevan. Hasil yang didapatkan adalah bahwa meskipun daun jati cina menunjukkan manfaat yang besar dalam menangani konstipasi maupun pembersihan usus, tetapi perlu diperhatikan juga bahwa penggunaannya yang berlebih berpotensi memberikan efek toksisitas pada manusia.

The Influence of Mycorrhizal Fungi on the Accumulation of Sennosides A and B in Senna alexandrina and Senna italica

Symbiotic arbuscular mycorrhizal fungi (AMF) play a major role in plant development, growth, and relationships with the environment through a change in the accumulation of secondary metabolites; hence, we planned to investigate AMF’s influence on sennoside A and B accumulation in Senna alexandrina (SA) and Senna italica (SI). Seeds of SA (S. alexandrina free of mycorrhizae) and SI (S. italica free of mycorrhizae) were planted in two types of soils: +mycorrhiza and—mycorrhiza. The plant leaves of SA, SI, S. alexandrina with mycorrhizae (SAM) and S. italica with mycorrhizae (SIM) were collected and extracted (with 85% methanol), and sennoside A and B content was evaluated by the HPLC–UV method. The antioxidant activity of SA, SI, SAM and SIM was evaluated by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods, while antimicrobial properties were evaluated by the minimum inhibitory concentration method (MIC). The AMF colonization was 85.66% and 85%, respectively, in the roots of SA and SI. The HPLC analysis showed a significant increase in (%) the content of sennoside A/sennoside B by 71.11/88.21, respectively, in SAM and 6.76/36.37 in SIM, which clearly indicated positive AMF effects. The DPPH/ABTS [The half maximal inhibitory concentration (IC50): 235.9/321.5 µg/mL] scavenging activity of SAM was comparatively higher and it also exhibited strong antibacterial action (MIC: 156.25 µg/mL), which supported the increase in sennoside content. This finding may be useful for further investigations of the symbiotic relation of mycorrhizal fungi with other plant species.

Ethnobotanical Studies of flora of Jebel Aulia district, Khartoum state with emphasis to toxicity of the common medicinal plants: دراسة تصنيفية للنباتات واستخداماتها العشبية في منطقة جبل أولياء، ولاية الخرطوم مع التركيز علي سمية النباتات الطبية المحلية

Ethnobotany is the scientific study of the relationships that exist between people and plants. Ethnobotanists aim to document, describe and explain complex relationships between cultures and plants, focusing primarily on how plants are used, managed and perceived across human societies. This includes use for food, clothing, currency, ritual, medicine, dye, construction, cosmetics. Ethnobotanical studies are often significant in revealing locally important plant species especially for the discovery of crude drugs The documentation of traditional knowledge, especially on the medicinal uses of plants. has provided many important drugs of modern day. Out of the total flowering plants reported from the world, more than 50, 000 are used for medicinal purposes ( Govaerts, 2001 ). Extensive and intensive field trips were conducted to the study area during a course of two years from 2013 to 2015 representing several sites through Jebel Aulia district, Khartoum state. The study revealed a total of 117 species belonging to 100 genera and 45 families were recorded at Jebel Aulia locality. The family Poaceae was found to be the richest (13 species) followed by Ceasalpiniaceae, Euphorbiaceae, Mimosaceae (7 species) and Asteraceae (6 species). Herbs have dominated the vegetation of the study area (62. 40%) followed with trees (15. 40%), shrubs (8. 5%). Genus Euphorbia was considered as the most speciose (4 species) pursued with Senna and Ipomea which were represented by 3 species each. Tribulus longipetalus and Cyperus alopecuroides along with their Ethnomedicinal uses were documented for the first time. Fruits were the most commonly used plant part to prepare medicine followed with leaves and seeds. The main preparation methods of herbal remedies were infusion (25%) followed with decoction (14. 3%), edible (10. 7%) and pills (10. 7%). Oral administration was the most common route of herbal remedies within traditional medicine. Urinary tracts, kidney and abdominal complaints were the most frequent ailments traditionally healed. The source of medicinal plants was wild, (41. 66%), were cultivated (33. 34%) and (25%) were commercially bought from the market. Senna alexandrina was the most cited and important plant in the study area followed with Ziziphus spina-christi, Acacia nilotica, Solenostemma argel and Cymbopogon proximus. High informants consensus factor was indicated for Medicinal, furniture and food uses. Ziziphus spina Christi, Acacia senegal, Allium sativum and Cinnamomum verum have highest use-value ranging from (0. 062 to 0. 05). Cytotoxicity was evaluated using plants with a high relative frequency of citation. The results revealed that LC50 of water and ethanolic extracts of Acacia nilotica were more than 1000 µg/ml which considered not toxic and consequently safe at this dose. Whereas, water and ethanolic extracts of Senna alexandrina were less than LC 50 =256. 206 - 409. 6079 µg/ml. which is considered moderately toxic. Therefore, Senna extracts should be carefully used.