Effects of outplanting time on growth, shedding and quality of Saccharina latissima (Phaeophyceae) in its northern distribution range

To reach the goal of large-scale seaweed cultivation in Norway and the rest of Europe, new knowledge about the commercially important kelp speciesSaccharina latissimais needed. Efforts to maximise biomass by outplanting the seaweed in different seasons can affect seaweed quality. Here, we investigate the effects of outplanting time (February, April, and May) when cultivatingS. latissimain the northern range of the species’ distribution. We studied the quantity and quality of the seaweed biomass produced in the autumn following outplanting. Effects on quantity were evaluated as seaweed frond area, relative daily growth rate (DGR) and relative daily shedding rate (DSR). Quality was evaluated by tissue content of carbon and nitrogen compounds and number of fouling epizoans. Cultivation was successful when seedlings were outplanted in both February and April, but not in May. An earlier outplanting, in February, gave a prolonged time for grow-out at sea prior to the main recruitment event of epizoans that occurred in September, thereby earlier outplanting resulted in larger frond areas. The frond area reached in September was doubled when seedlings were outplanted in February compared to April, whereas a later outplanting in April gave a higher DGR and DSR, higher carbon content, and lower amount of fouling epizoans. The outplanting season did not affect tissue nitrate concentration or internally stored nitrate. These results show that outplanting time is an important factor to consider especially for biomass yield, but also for seaweed quality, including epibiosis of the seaweed biomass.

A test of the provisioning hypothesis of recruitment control in Georges Bank haddock

The haddock (Melanogrammus aeglefinus) stock of the Georges Bank region of the US Northeast Continental Shelf displays a pattern of large, episodic recruitments. Among the hypothesized controlling mechanisms is the idea that recruitment events are related to provisioning of pre-spawning haddock by the fall bloom the year before. With the occurrence of a recent large recruitment event in 2013, it would be prudent to retest this hypothesis. Fall bloom magnitude was positively correlated (r=0.645, p=0.005) with haddock survivor ratio (recruits per spawning biomass) including data from the 2013 recruitment. This relationship identifies a pathway of bottom up control of a resource species, thus focusing concern over recent changes in lower trophic level productivity.

The autophagy adaptor NDP52 and the FIP200 coiled-coil allosterically activate ULK1 complex membrane recruitment

The selective autophagy pathways of xenophagy and mitophagy are initiated when the adaptor NDP52 recruits the ULK1 complex to autophagic cargo. Hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) was used to map the membrane and NDP52 binding sites of the ULK1 complex to unique regions of the coiled coil of the FIP200 subunit. Electron microscopy of the full-length ULK1 complex shows that the FIP200 coiled coil projects away from the crescent-shaped FIP200 N-terminal domain dimer. NDP52 allosterically stimulates membrane-binding by FIP200 and the ULK1 complex by promoting a more dynamic conformation of the membrane-binding portion of the FIP200 coiled coil. Giant unilamellar vesicle (GUV) reconstitution confirmed that membrane recruitment by the ULK1 complex is triggered by NDP52 engagement. These data reveal how the allosteric linkage between NDP52 and the ULK1 complex could drive the first membrane recruitment event of phagophore biogenesis in xenophagy and mitophagy.

The autophagy adaptor NDP52 and the FIP200 coiled-coil allosterically activate ULK1 complex membrane recruitment

The selective autophagy pathways of xenophagy and mitophagy are initiated when the adaptor NDP52 recruits the ULK1 complex to autophagic cargo. Hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) was used to map the membrane and NDP52 binding sites of the ULK1 complex to unique regions of the coiled coil of the FIP200 subunit. Electron microscopy of the full-length ULK1 complex shows that the FIP200 coiled coil projects away from the crescent-shaped FIP200 N-terminal domain dimer. NDP52 allosterically stimulates membrane-binding by FIP200 and the ULK1 complex by promoting a more dynamic conformation of the membrane-binding portion of the FIP200 coiled coil. Giant unilamellar vesicle (GUV) reconstitution confirmed that membrane recruitment by the ULK1 complex is triggered by NDP52 engagement. These data reveal how the allosteric linkage between NDP52 and the ULK1 complex could drive the first membrane recruitment event of phagophore biogenesis in xenophagy and mitophagy.

Molecular basis for three-dimensional elaboration of the Aquilegia petal spur

By enforcing specific pollinator interactions, Aquilegia petal nectar spurs maintain reproductive isolation between species. Spur development is the result of three-dimensional elaboration from a comparatively two-dimensional primordium. Initiated by localized, oriented cell divisions surrounding the incipient nectary, this process creates a pouch that is extended by anisotropic cell elongation. We hypothesized that the development of this evolutionary novelty could be promoted by non-mutually exclusive factors, including (i) prolonged, KNOX-dependent cell fate indeterminacy, (ii) localized organ sculpting and/or (iii) redeployment of hormone-signalling modules. Using cell division markers to guide transcriptome analysis of microdissected spur tissue, we present candidate mechanisms underlying spur outgrowth. We see dynamic expression of factors controlling cell proliferation and hormone signalling, but no evidence of contribution from indeterminacy factors. Transcriptome dynamics point to a novel recruitment event in which auxin-related factors that normally function at the organ margin were co-opted to this central structure. Functional perturbation of the transition between cell division and expansion reveals an unexpected asymmetric component of spur development. These findings indicate that the production of this three-dimensional form is an example of organ sculpting via localized cell division with novel contributions from hormone signalling, rather than a product of prolonged indeterminacy.

Recruitment of Phalaris aquatica within existing swards. 1. Effects of biomass manipulation, seed level modification and site preparation

A successful recruitment event in perennial grasslands is infrequent and when it occurs, the rate of recruitment and survival is low, <1% in most occasions. This paper reports on two field experiments that investigate the effects of biomass manipulation, seed level modification and site preparation on the recruitment of Phalaris aquatica seedlings. The experiments were done through drier than average years, where P. aquatica achieved successful recruitment of seedlings. Recruitment rates proportional to total seed set were 1.3–13.3% in experiment 1 and 0.5–4.2% in experiment 2. The control treatment, on average, resulted in 352 seedlings/m2 in experiment 1 and 16/m2 in experiment 2 compared with the best treatments which had 500 and 38/m2, respectively, on average. There was poor seed set in experiment 2 before the recruitment event. Presence of existing biomass compared with either removing or leaving the plant material on the ground had greater success on seedling emergence, whereas seed addition had little effect suggesting that microsites may be more important than seed availability for P. aquatica seedling emergence. Soil scarification in general failed to have significant effects in both experiments. Seedlings survived until the following summer, but few then remained through the ensuing drought. This research showed that minimal intervention was needed to encourage emergence and early survival, and provides data on the mechanisms involved for recruitment of a perennial grass species in existing swards.