Identifikasi Standarisasi Laboratorium IPA di Salah Satu MTs Jember

Laboratorium sekolah yang baik harus memenuhi kelengkapan peralatan dan memperhatikan fasilitas penunjang sebagaimana yang ditetapkan oleh Permendiknas No. 24 Tahun 2007 tentang standarisasi laboratorium. Tujuan penelitian ini adalah untuk mengidentifikasi kelayakan dan optimalisasi laboratorium IPA yang terdapat di salah satu MTs yang ada di Kabupaten Jember. Penelitiaan ini berjenis peneltian deskriptif kualitatif. Sumber data yang digunakan berasal dari hasil wawancara. Sumber data primer adalah salah satu guru yang ada di MTs X dan sumber data sekunder berasal dari berbagai artikel jurnal nasional. Teknik pengumpulan data menggunakan teknik wawancara bebas terpimpin. Hasil wawancara menginformasikan bahwa kegiatan praktikum yang dilaksanakan di salah satu sekolah MTs Jember masih belum terlaksana dengan baik. Peralatan yang terdapat di laboratorium MTs X bisa dikatakan lengkap, termasuk ketersediaan KIT fisika. Dari hasil data yang diperolah dapat disimpulkan bahwa laboratorium yang terdapat disekolah tersebut kurang dioptimalkan dengan baik karena beberapa hal seperti guru yang mengajar di salah satu MTs X bukan berasal dari background bidang studi IPA, kemudian sulitnya mendapatkan perizinan dari pihak yayasan untuk menggunakan laboratorium karena takut alat-alat laboratorium menjadi rusak. Kata Kunci : laboratorium, pembelajaran IPA, praktikum A good school laboratory must meet the completeness of equipment and pay attention to supporting facilities as stipulated by Permendiknas No. 24 of 2007 concerning laboratory standardization. The purpose of this study was to identify the feasibility and optimization of the science laboratory in one of the MTs in Jember Regency. This research is a qualitative descriptive research. The source of the data used comes from the results of interviews. The primary data source is one of the teachers in MTs X and the secondary data sources are from various national journal articles. Data collection techniques using guided free interview techniques. The results of the interview informed that the practicum activities carried out in one of the MTs Jember schools were still not carried out properly. The equipment in the MTs X laboratory can be said to be complete, including the availability of a physics KIT. From the results of the data obtained, it can be concluded that the laboratory in the school is not optimized properly because several things, such as the teacher who teaches at one MTs X is not from a science background, then it is difficult to get permission from the foundation to use the laboratory for fear of tools. -Laboratory equipment is damaged. Keywords: science learning, laboratory, practicum.

Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP) is an effective alternative for SARS-CoV-2 molecular detection in middle-income countries

ABSTRACTMolecular diagnosis of SARS-CoV-2 in developing countries is still a big challenge. The reference standard, RT-qPCR, recommended by WHO, is not widely available, difficulting early identification of cases. Furthermore, the transport logistic between the sample collection point and the laboratory facilities can alter the samples, producing false negative results. RT-LAMP is a cheaper, simpler molecular technique that can be an interesting alternative to be offered in hospital laboratories. We present the evaluation of a RT-LAMP for diagnosis of SARS-CoV-2 in two steps: the laboratory standardization and the clinical validation, comparing it with the standard RT-qPCR. In the standardization phase, limit of detection and robustness values were obtained using RNA from a Peruvian SARS-CoV-2 strain. It presented 100% agreement between triplicates (RT-LAMP agreement with all RT-qPCR reactions that presented Ct ≤ 30) and robustness (RT-LAMP successful reactions with 80% reaction volume and 50% primer concentration). 384 nasal and pharyngeal swabs collected from symptomatic patients and stored in the INS biobank were tested and we obtained 98.75%, 87.41%, 97.65% and 92.96% for specificity, sensitivity, positive predictive value and negative predictive values respectively. Then, 383 samples from symptomatic patients with less than 15 days of disease, were tested both with the RT-LAMP and with the RT-qPCR, obtaining e 98.8%, 88.1%, 97.7% y 93.3% of specificity, sensitivity, positive predictive value and negative predictive values respectively. The laboratory standardization and the clinical validation presented the same value by Kappa-Cohen index (0.88) indicating an almost perfect agreement between RT-LAMP and RT-qPCR for molecular detection of SARS-CoV-2. We conclude that this RT-LAMP protocol presented high diagnostic performance values and can be an effective alternative for COVID-19 molecular diagnosis in hospitals, contributing to early diagnosis and reducing the spread of virus transmission in the Peruvian population.