Characterization of the western white pine TIR-NBS-LRR (PmTNL2) gene by transcript profiling and promoter analysis

Genome ◽  
2019 ◽  
Vol 62 (7) ◽  
pp. 477-488
Author(s):  
Jun-Jun Liu ◽  
Yu Xiang
Keyword(s):  
Plant Disease Resistance ◽  
Growth And Development ◽  
Expression Patterns ◽  
In Silico Analysis ◽  
Regulatory Elements ◽  
Specific Pattern ◽  
Pcr Analysis ◽  
Pinus Monticola ◽  
White Pine ◽  
Western White Pine

Proteins with nucleotide-binding site (NBS) and leucine-rich repeats (LRRs) have been reported to play important roles in plant disease resistance, growth, and development. However, no comprehensive analysis of this protein family has been performed in conifers. Here we report that the Pinus monticola PmTNL2 gene is a member of the NBS-LRR superfamily. Quantitative reverse transcription-PCR (qRT-PCR) analysis revealed that the PmTNL2 transcript was expressed in a tissue-specific pattern with extensive regulation by various environmental stimuli in western white pine seedlings, suggesting its wide involvement in stress defense and diverse developmental processes. In silico analysis of the PmTNL2 promoter region revealed multiple cis-regulatory elements characterized with potential functions for development-, light-, and stress-regulated transcript expression. Expression patterns were largely confirmed by PmTNL2 promoter-directed reporter gene expression using stable transgenic Arabidopsis plants. Notably, the PmTNL2 promoter activity was highly expressed in shoot apical and floral meristems and was induced strongly with vascular specificity by pathogen infection. Our data has provided a fundamental insight into both expression regulation and putative functions of the PmTNL2 gene in the context of plant growth and development, as well as in responses to environmental stressors. Promoter application as a potential tool for tree improvement was further discussed.

Antifungal activity of a Pinus monticola antimicrobial peptide 1 (Pm-AMP1) and its accumulation in western white pine infected with Cronartium ribicola

2011 ◽  
Vol 57 (8) ◽  
pp. 667-679 ◽  
Author(s):  
Arezoo Zamany ◽  
Jun-Jun Liu ◽  
Abul Ekramoddoullah ◽  
Richard Sniezko
Keyword(s):  
Antifungal Activity ◽  
Antimicrobial Peptide ◽  
Regulatory Elements ◽  
Post Treatment ◽  
Protein Accumulation ◽  
Cronartium Ribicola ◽  
Pinus Monticola ◽  
White Pine ◽  
Significant Difference ◽  
Western White Pine

Pinus monticola antimicrobial peptide 1 (Pm-AMP1) was expressed and purified from bacterial cell lysate and its identity and purity confirmed by Western blot analysis using the Pm-AMP1 antibody. Application of Pm-AMP1 resulted in visible hyphal growth inhibition of Cronartium ribicola , Phellinus sulphurascens , Ophiostoma montium , and Ophiostoma clavigerum 3–12 days post-treatment. Pm-AMP1 also inhibited spore germination of several other phytopathogenic fungi by 32%–84% 5 days post-treatment. Microscopic examination of C. ribicola hyphae in contact with Pm-AMP1 showed distinct morphological changes. Seven western white pine ( Pinus monticola Douglas ex D. Don) families (Nos. 1, 2, 5, 6, 7, 8, 10) showing partial resistance to C. ribicola in the form of bark reaction (BR) were assessed by Western immunoblot for associations between Pm-AMP1 accumulation and family, phenotype, canker number, and virulence of C. ribicola. There was a significant difference (p < 0.001) in mean Pm-AMP1 protein accumulation between families, with higher levels detected in the full-sib BR families (Nos. 1, 2, 5) than the half-sib BR families (Nos. 6, 7). Family 8, previously described as a Mechanism ‘X’ BR family, had the highest number of BR seedlings and displayed high Pm-AMP1 levels, whereas the susceptible family (No. 10) showed the lowest levels (p < 0.05). Family 1 showed a significant association between Pm-AMP1 accumulation and overall seedling health (p < 0.01, R = 0.533), with higher protein levels observed in healthy versus severely infected seedlings. In general, low Pm-AMP1 levels were observed with an increase in the number of cankers per seedling (p < 0.05), and seedlings inoculated with the avirulent source of C. ribicola showed significantly higher Pm-AMP1 levels (p < 0.05) in the majority of BR families. Cis-acting regulatory elements, such as CCAAT binding factors, and an AG-motif binding protein were identified in the Pm-AMP1 promoter region. Multiple polymorphic sites were identified within the 5′ untranslated region and promoter regions. Our results suggest that Pm-AMP1 is involved in the western white pine defense response to fungal infection, as observed by its antifungal activity on C. ribicola and a range of phytopathogens as well as through its association with different indicators of resistance to C. ribicola.

Western white pine development in relation to biophysical characteristics across different spatial scales in the Coeur d'Alene River basin in northern Idaho, U.S.A.

2002 ◽  
Vol 32 (7) ◽  
pp. 1109-1125 ◽  
Author(s):  
Theresa B Jain ◽  
Russell T Graham ◽  
Penelope Morgan
Keyword(s):  
River Basin ◽  
Spatial Scales ◽  
Pinus Monticola ◽  
White Pine ◽  
Basal Diameter ◽  
Landscape Characteristics ◽  
Coeur D'alene ◽  
Biophysical Factors ◽  
Western White Pine ◽  
Northern Idaho

Many studies have assessed tree development beneath canopies in forest ecosystems, but results are seldom placed within the context of broad-scale biophysical factors. Mapped landscape characteristics for three watersheds, located within the Coeur d'Alene River basin in northern Idaho, were integrated to create a spatial hierarchy reflecting biophysical factors that influence western white pine (Pinus monticola Dougl. ex D. Don) development under a range of canopy openings. The hierarchy included canopy opening, landtype, geological feature, and weathering. Interactions and individual-scale contributions were identified using stepwise log–linear regression. The resulting models explained 68% of the variation for estimating western white pine basal diameter and 64% for estimating height. Interactions among spatial scales explained up to 13% of this variation and better described vegetation response than any single spatial scale. A hierarchical approach based on biophysical attributes is an excellent method for studying plant and environment interactions.

scholarly journals Evolutionary Understanding of Metacaspase Genes in Cultivated and Wild Oryza Species and Its Role in Disease Resistance Mechanism in Rice

Genes ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 1412
Author(s):  
Ruchi Bansal ◽  
Nitika Rana ◽  
Akshay Singh ◽  
Pallavi Dhiman ◽  
Rushil Mandlik ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Wild Rice ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Resistance Mechanism ◽  
Regulatory Elements ◽  
Stress Conditions ◽  
Pcr Analysis ◽  
North East ◽  
Wild Rice Species

Metacaspases (MCs), a class of cysteine-dependent proteases found in plants, fungi, and protozoa, are predominately involved in programmed cell death processes. In this study, we identified metacaspase genes in cultivated and wild rice species. Characterization of metacaspase genes identified both in cultivated subspecies of Oryza sativa, japonica, and indica and in nine wild rice species was performed. Extensive computational analysis was conducted to understand gene structures, phylogenetic relationships, cis-regulatory elements, expression patterns, and haplotypic variations. Further, the haplotyping study of metacaspase genes was conducted using the whole-genome resequencing data publicly available for 4726 diverse genotype and in-house resequencing data generated for north-east Indian rice lines. Sequence variations observed among wild and cultivated rice species for metacaspase genes were used to understand the duplication and neofunctionalization events. The expression profiles of metacaspase genes were analyzed using RNA-seq transcriptome profiling in rice during different developmental stages and stress conditions. Real-time quantitative PCR analysis of candidate metacaspase genes in rice cultivars Pusa Basmati-1 in response to Magnaporthe oryzae infection indicated a significant role in the disease resistance mechanism. The information provided here will help to understand the evolution of metacaspases and their role under stress conditions in rice.

scholarly journals The Transferability of Western White Pine to and within British Columbia Based on Early Survival, Environmental Damage, and Juvenile Height

1999 ◽  
Vol 14 (1) ◽  
pp. 41-47 ◽  
Author(s):  
M. D. Meagher ◽  
R. S. Hunt
Keyword(s):  
Root Rot ◽  
Source Parameters ◽  
Test Series ◽  
Environmental Damage ◽  
Pinus Monticola ◽  
White Pine ◽  
Spring Frost ◽  
Provenance Test ◽  
Late Spring Frost ◽  
Western White Pine

Abstract Survival, environmental damage, and juvenile height of 27 provenances of western white pine (Pinus monticola) in three plantation series were analyzed after 5 to 13 yr on site to assist in evaluating seed-transfer practices. Survival averaged 79.2% on nine sites in the "root-rot" series and 84.1% on six sites in the "provenance-test" series. Trends of survival on seed-source parameters differed between series, generally increasing with both elevation and latitude in the root-rot series, while generally decreasing with latitude in the provenance-test series. Analysis by seed zone (coast or interior) and plantation region (coast, southern interior, or northern interior) showed that coastal sources on interior sites caused much of these anomalies. Substantial environmental damage was found only on sites near to or beyond the species' northern limit. Differences in the trend of damage with source parameters were found between the test series: interior sources were damaged less than coastal sources on two root-rot sites, whereas interior sources were damaged more heavily than coastal sources on the provenance-test site exhibiting substantial damage. Damage increased with increasing provenance latitude and elevation in the root-rot series, while it dropped with increasing elevation in the "provenance-test" series. In general, taller seedlings in taller provenances were damaged. Coastal seed should not be used on interior sites, but transfer of seed from the BC interior to the BC coast seems safe. We recommend that the present limits for latitudinal transfer be doubled, except where late-spring-frost risk is high, and that elevational transfer of seeds for the interior zone be reduced by about half West. J. Appl. For. 14(1)41-47.

scholarly journals White Pine Blister Rust in British Columbia III. Effects on the Gene Pool of Western White Pine

1985 ◽  
Vol 61 (6) ◽  
pp. 484-488 ◽  
Author(s):  
R. S. Hunt ◽  
J. F. Manville ◽  
E. von Rudloff ◽  
M. S. Lapp
Keyword(s):  
Pacific Northwest ◽  
Gene Pool ◽  
Resistance Mechanisms ◽  
Geographic Range ◽  
White Pine Blister Rust ◽  
Pinus Monticola ◽  
White Pine ◽  
Blister Rust ◽  
The Pacific ◽  
Western White Pine

Cluster analyses of relative terpene abundance in foliage of western white pine (Pinus monticola Dougl.) trees from throughout the Pacific Northwest geographic range of the species were produced. Terpene patterns were randomly distributed among populations; no geographic or site trends were evident. Although blister rust is devastating to stands, the gene pool is widely distributed and may well be preserved without establishing gene banks.About 40-50 trees selected at random would yield offspring with nearly all possible terpene patterns characteristic of the species and would thus constitute a broad genetic base. Therefore seed orchards do not necessarily need to be composed of many individuals, rather, they should contain highly selected individuals with multiple desirable traits including multiple blister rust resistance mechanisms. Key words: terpenes, dendrogram

scholarly journals Height growth determinants and adaptation to temperature in pines: a case study of Pinus contorta and Pinus monticola

2006 ◽  
Vol 36 (5) ◽  
pp. 1059-1066 ◽  
Author(s):  
Isabelle Chuine ◽  
Gerald E Rehfeldt ◽  
Sally N Aitken
Keyword(s):  
Growth Performance ◽  
Pinus Contorta ◽  
Lodgepole Pine ◽  
Shoot Elongation ◽  
Height Growth ◽  
Pinus Monticola ◽  
White Pine ◽  
Total Height ◽  
Geographical Regions ◽  
Western White Pine

In this study we aimed to compare and explain the height growth performance of two contrasting pine species: lodgepole pine (Pinus contorta Dougl. ex. Loud) and western white pine(Pinus monticola Dougl. ex D. Don.). We compiled measurements of total height growth at different ages and shoot elongation phenology realized in several provenance test trials for 109 provenances of lodgepole pine and 54 provenances of western white pine. The response of shoot elongation to temperature was assessed using a phenological model fitted on provenance mean growth curves. Although total height growth followed the same geographic trends in both species, the response of shoot elongation to temperature was different between the two, with few (lodgepole pine) or no differences among provenances (western white pine) from diverse geographic regions. The temperature for which potential cell growth rate is 50% was 10.8 ± 0.13 °C (mean ± standard error) for western white pine compared to 5.26 ± 0.075 °C for lodgepole pine. Phenology did not explain growth performance differences among geographical regions in both species, which instead were explained by differences in the number of internodes set the preceding summer; provenances originating from stressful environments produced the fewest internodes, possibly due to reallocation of carbohydrates to stress resistance.

Western white pine growth relative to forest openings

2004 ◽  
Vol 34 (11) ◽  
pp. 2187-2198 ◽  
Author(s):  
Theresa B Jain ◽  
Russell T Graham ◽  
Penelope Morgan
Keyword(s):  
Competitive Advantage ◽  
Polynomial Regression ◽  
Timber Harvesting ◽  
Tree Age ◽  
Pinus Monticola ◽  
White Pine ◽  
Canopy Opening ◽  
Western White Pine ◽  
Sapling Growth ◽  
Log Linear

In northern Rocky Mountains moist forests, timber harvesting, fire exclusion, and an introduced stem disease have contributed to the decline in western white pine (Pinus monticola Dougl. ex D. Don) abundance (from 90% to 10% of the area). Relations between canopy openings (0.1–15 ha) and western white pine growth within different physical settings are identified. Objectives include relating western white pine seedling and sapling growth to canopy opening attributes (defined by fisheye photography), identifying western white pine competitive thresholds (occupancy, competitive advantage, free-to-grow status) in relation to opening size, and relating canopy opening attributes to overstory density descriptors. We sampled 620 western white pine plus competing trees, canopy opening characteristics, landscape position, and overstory density descriptors. Analysis included log-linear and polynomial regression. Visible sky (canopy opening) and tree age were significantly related to growth of selected pines. Radiation explained less variation in growth than canopy opening. Thresholds for western white pine to occupy a site (>23% canopy opening), gain a competitive advantage (>50% canopy opening), and achieve free-to-grow status (>92% canopy opening) over western hemlock were developed. There was a significant although weak (R2 < 0.12) relation between canopy opening and overstory density descriptors.

PINUS MONTICOLA, WESTERN WHITE PINE

2014 ◽  
pp. 1022-1025
Author(s):  
Henry John Elwes ◽  
Augustine Henry
Keyword(s):  
Pinus Monticola ◽  
White Pine ◽  
Western White Pine
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