Influence of Altitude and Environmental Temperature on Muscle Functional and Mechanical Activation After 30' Time Trial Run

The purpose of this study was to explore the influence of altitude and environmental temperature on muscle mechanical and functional activation after 30' Time Trial run (30' TT). Twenty physical active males (Age= 20.4 ±3.21 years, VO2max= 47.2 ±5.2 ml/kg/min) performed a 30' TT in three different conditions of altitude and temperature: Control Condition [CC] (1137 m.a.s.l. at 26 ±1.5°C), Heat-Low Condition [HLC] (3 m.a.s.l. at 30.5 ±0.6°C), and Cool-High Condition [CHC] (2369 m.a.s.l. at 14.2 ±0.6°C). Tensiomyography (TMG), Countermovement Jump (CMJ), and Delayed Onset Muscle Soreness (DOMS) were measured pre and post running. During the 30' TT, distance, speed, heart rate (HR), rate of perceived exertion (RPE), and thermal index (WBGT) were measured. Results show a significant decrease in body weight and a significant increase in DOMS and jump height in CMJ after running in each condition. TMG responses increased maximum radial muscle displacement (Dm) and decreased muscle contraction time (Tc) both rectus and biceps femoris muscles after running. During the 30' TT, the results did not show a significant difference in RPE, HR, distance, and speed among conditions. In conclusion, altitude and temperature seem not to significantly affect the mechanical and functional responses 30' TT, despite the tendency of HLC to decrease both activation and performance.

Reduced muscle contractile function in elite young soccer players after a short-congested fixture period

Soccer is a very popular sport, which has been widely investigated. However, little is known about the effects of congested fixture periods on muscle mechanical responses since access to elite teams during competitive periods is highly restricted. Thus, the goal of this study was to describe the effects of a short-congested fixture period on the neuromechanical parameters related to fatigue and stiffness of elite young soccer players. Nineteen young elite male soccer players (age 17.13 ± 0.51 years, body mass 69.00 ± 1.22 kg, and height 174.09 ± 0.84 cm) participated in the study. The players took part in a preparatory tournament for the FIFA® U-17 World Cup, composed of a total of six matches in 1 week. Pre- and post-tensiomyographic changes were assessed by means of the normalized response speed (Vrn) and other tensiomyographic markers related to muscle fatigue (i.e. maximum radial muscle displacement (Dm) and velocities of contraction at 10% and 90% (V10 and V90) of both legs in two muscles (biceps femoris and rectus femoris). The players presented a possible to likely impairment in Dm, V10, and V90 of both legs and muscles, with the exception of the non-dominant biceps femoris. Vrn did not vary in any condition, although it presented a slight tendency to decrease. The data demonstrated impairments in the tensiomyographic markers in both legs and muscles, with the exception of the non-dominant biceps femoris. A short-congested fixture period in young soccer players leads to a decrease in muscle stiffness due to cumulative fatigue and lack of muscle recovery, this despite the efforts made by the medical staff to counteract the fatigue caused by the games. The use of tensiomyographic markers could be advantageous for rapidly monitoring neuromechanical muscle impairments throughout competitive soccer periods.

Neuromuscular Parameters Predict the Performance in an Incremental Cycling Test

The aim was to determine the predictive capacity of neuromuscular parameters on physiological predictors of performance related to pedaling power. The sample comprised fifty elite cyclists. On the same day, they were given a neuromuscular evaluation with tensiomyography (TMG) and then performed an effort test on a cycle ergometer until exhaustion. The TMG recorded the maximum radial muscle belly displacement, contraction time, delay time, derivative normalized response speed, and lateral symmetry. Peak power output (Wpeak·kg−1), effort time, maximum lactate concentration, power in the first lactate threshold, and power in the second lactate threshold were recorded in the effort test. Linear regression analysis was used to determine the explanatory capacity of neuromuscular parameters on potential cycling performance indicators. A higher Wpeak·kg−1 during a maximal incremental test on the cycle ergometer can be predicted moderately (R2=0.683; R2a=0.615; R=0.826; Std. Error=0.26017; p<0.001) by a longer rectus femoris contraction time and a greater radial muscle belly displacement of the vastus lateralis and vastus medialis as well as a slower normalized response speed of the biceps femoris. In conclusion, neuromuscular parameters can partially explain performance in a specific cycling test until exhaustion.

Ultrastructure of the postcorpus of the esophagus of Teratocephalus lirellus (Teratocephalida) and its use for interpreting character evolution in Secernentea (Nematoda)

The ultrastructure of the postcorpus of the putative outgroup of Secernentea (Nematoda), Teratocephalus lirellus (Teratocephalida), is compared with previous observations of representative species Zeldia punctata (Cephalobina), Caenorhabditis elegans (Rhabditina), and Diplenteron sp. (Diplogastrina) in order to interpret the evolution of feeding structures within Secernentea. The postcorpus of T. lirellus consists of 6 marginal, 13 muscle, 3 gland, and 11 nerve cells. In both T. lirellus and Z. punctata, one duct from each of two subventral glands opens into the esophageal lumen at the junction of the isthmus and the basal bulb, whereas in C. elegans and Diplenteron sp., homologous openings are at the posterior end of the median bulb. Caenorhabditis elegans and Z. punctata each have two additional glands that open within the basal bulb. The postcorpus of each taxon has four anterior-to-posterior layered sets of radial muscle cells, except in Diplenteron sp., which lacks a grinder and has homologs to the anterior two sets only. The anterior set of muscles of T. lirellus and Z. punctata includes six mononucleate cells, whereas the homolog in C. elegans and Diplenteron sp. includes three binucleate cells. Evaluation of character polarity defines Rhabditina and Diplogastrina as sister taxa, and suggests that the character of five glands may result from functional convergence.

Description of growth in the tropical cuttlefish Sepia elliptica using muscle tissue

The mantle muscle tissue of cuttlefish is predominantly made up of circular muscle fibres which run around the body. This circular muscle tissue is divided into discrete blocks by radial fibres, which run perpendicular to the main axis of the body. Growth of mantle muscle tissue was described in the tropical cuttlefish Sepia elliptica by examining muscle block size and the density and size distribution of muscle fibres. Changes in these variables were assessed in juvenile and adult cuttlefish. Generation of new muscle fibres, both within new and existing muscle blocks, occurred as animals grew across the size range. Anterior muscle blocks were smaller than muscle blocks in the middle and posterior regions of the mantle, suggesting that new blocks were generated anteriorly. Muscle blocks were also created in the middle and posterior regions by division of existing large blocks into small blocks by generation of circular and radial muscle fibres. The examination of cuttlefish muscle growth dynamics provided an alternative way of assessing cephalopod growth mechanisms.

Actions of the anthelmintic ivermectin on the pharyngeal muscle of the parasitic nematode, Ascaris suum

The anthelmintic ivermectin has a number of effects on nematodes which result in changes in behaviour, particularly locomotion, including paralysis and an inhibition of feeding. This paper describes the application of an in vitro pharmacological approach to further delineate the action of ivermectin on feeding behaviour. Contraction of Ascaris suum pharyngeal muscle was monitored using a modified pressure transducer system which detects changes in intrapharyngeal pressure and therefore contraction of the radial muscle of the pharynx. The pharynx did not contract spontaneously. However, serotonin (5-HT, 100 μm) stimulated rhythmic contractions and relaxations (pumping) at a frequency of 0·5 Hz. γ-Aminobutyric acid (GABA) and glutamic acid inhibited the pumping elicited by 5-HT. The duration of inhibition was concentration dependent (1–1000 μm) with a threshold of 1 μm and 10 μm respectively (n=8). Ivermectin also inhibited pharyngeal pumping (1–1000 nM). At lower concentrations, ivermectin (1–10 pM) potentiated the GABA and glutamate inhibition, so that inhibition occurred at concentrations which were below threshold in the absence of ivermectin. These data provide evidence that the pharynx is a site for the action of ivermectin. Thus interruption of pharyngeal processes such as, feeding, regulation of hydrostatic pressure and secretion may provide a new site of anthelmintic action.