Quantifying the glacial meltwater contribution to streams in mountainous regions using highly resolved stable water isotope measurements

This study aims to determine the contribution of glacial meltwater to streams in mountainous regions based on stable water isotope measurements (δ18O and δ2H). For this purpose, three partially glaciated catchments were selected as the study area in the central Swiss Alps being representative of catchments that are used for hydropower energy production in Alpine regions. The glacial meltwater contribution to the catchments’ stream discharges was evaluated based on high-resolution δ18O and δ2H measurements of the end-members that contribute to the stream discharge (ice, rain, snow) and of the discharging streams. The glacial meltwater contribution to the stream discharges could be unequivocally quantified after the snowmelt in August and September when most of the annual glacial meltwater discharge occurs. In August and September, the glacial meltwater contribution to the stream discharges corresponds to up to 95 ± 2 % and to 28.7 % ± 5 % of the total annual discharge in the evaluated catchments. The high glacial meltwater contribution demonstrates that the mountainous stream discharges in August and September will probably strongly decrease in the future due to global warming-induced deglaciation, which will be, however, likely compensated by higher discharge rates in winter and spring. Nevertheless, the changing mountainous streamflow regimes in the future will pose a challenge for hydropower energy production in the mountainous areas. Overall, this study provides a successful example of an Alpine catchment monitoring strategy to quantify the glacial meltwater contribution to stream discharges based on stable isotope water data, which leads to a better validation of existing modelling studies and which can be adapted to other mountainous regions.

Modification of methane oxidation pathways during long-term incubations of methanic lake sediments

Anaerobic oxidation of methane (AOM) is one of the major processes limiting the release of the greenhouse gas methane from natural environments. In Lake Kinneret sediments, iron-coupled AOM (Fe-AOM) was suggested to play a substantial role (10–15 % relative to methanogenesis) in the methanic zone (> 20 cm sediment depth), based on geochemical profiles and experiments on fresh sediments. Apparently, the oxidation of methane is mediated by a combination of mcr gene bearing archaea and aerobic bacterial methanotrophs. Here we aimed to investigate the survival of this complex microbial interplay under controlled conditions. We followed the AOM process during long-term (~18 months) anaerobic slurry experiments of these methanic sediments with two stages of incubations and additions of 13C-labeled methane, multiple electron acceptors and inhibitors. After these incubation stages carbon isotope measurements in the dissolved inorganic pool still showed considerable AOM (3–8 % relative to methanogenesis). Specific lipid carbon isotope measurements and metagenomic analyses indicate that after the prolonged incubation aerobic methanotrophic bacteria were no longer involved in the oxidation process, whereas mcr gene bearing archaea were most likely responsible for oxidizing the methane. Humic substances and iron oxides are likely electron acceptors to support this oxidation, whereas sulfate, manganese, nitrate, and nitrite did not support the AOM in these methanic sediments. Our results suggest in the natural lake sediments methanotrophic bacteria are responsible for part of the methane oxidation by the reduction of combined micro levels of oxygen and iron oxides in a cryptic cycle, while the rest of the methane is converted by reverse methanogenesis. After long-term incubation, the latter prevails without bacterial methanotropic activity and with a different iron reduction pathway.

Ecohydrological travel times derived from in situ stable water isotope measurements in trees during a semi-controlled pot experiment

Tree water uptake processes and ecohydrological travel times have gained more attention in recent ecohydrological studies. In situ measurement techniques for stable water isotopes offer great potential to investigate these processes but have not been applied much to tree xylem and soils so far. Here, we used in situ probes for stable water isotope measurements to monitor the isotopic signatures of soil and tree xylem water before and after two deuterium-labeled irrigation experiments. To show the potential of the method, we tested our measurement approach with 20-year-old trees of three different species (Pinus pinea, Alnus incana and Quercus suber). They were planted in large pots with homogeneous soil in order to have semi-controlled experimental conditions. Additional destructive sampling of soil and plant material allowed for a comparison between destructive (cryogenic vacuum extraction and direct water vapor equilibration) and in situ isotope measurements. Furthermore, isotope-tracer-based ecohydrological travel times were compared to travel times derived from sap flow measurements. The time to first arrival of the isotope tracer signals at 15 cm stem hight were ca. 17 h for all tree species and matched well with sap-flow-based travel times. However, at 150 cm stem height tracer-based travel times differed between tree species and ranged between 2.4 and 3.3 d. Sap-flow-based travel times at 150 cm stem hight were ca. 1.3 d longer than tracer-based travel times. The isotope signature of destructive and in situ isotope measurements differed notably, which suggests that the two types of techniques sampled water from different pools. In situ measurements of soil and xylem water were much more consistent between the three tree pots (on average standard deviations were smaller by 8.4 ‰ for δ2H and by 1.6 ‰ for δ18O for the in situ measurements) and also among the measurements from the same tree pot in comparison to the destructive methods (on average standard deviations were smaller by 7.8 ‰ and 1.6 ‰ for δ2H and δ18O, respectively). Our study demonstrates the potential of semi-controlled large-scale pot experiments and very frequent in situ isotope measurements for monitoring tree water uptake and ecohydrological travel times. It also shows that differences in sampling techniques or sensor types need to be considered when comparing results of different studies and within one study using different methods.