Plant-associated microbiomes promote nutrient turnover in impoverished substrates of a biodiversity hotspot

The substrates of the Brazilian campos rupestres have extremely low concentrations of key nutrients, mainly phosphorus, imposing severe restrictions to plant growth. Regardless, this ecosystem harbors enormous biodiversity which raises the question of how nutrients are cycled and acquired by the biosphere. To uncover the nutrient turnover potential of plant-associated microorganisms in the campos rupestres, we investigated the compositions and functions of microbiomes associated with two species of the Velloziaceae family that grow over distinct substrates (soil and rock). Amplicon, metagenomic, and metagenome-assembled genome sequence data showed that the campos rupestres harbor a novel assemblage of plant-associated prokaryotes and fungi. Compositional analysis revealed that the plant-associated soil and rock communities differed in taxonomic structure but shared a core of highly efficient colonizers that were strongly coupled with nutrient mobilization. Investigation of functional and abundance data revealed that the plant hosts actively recruit communities by exuding organic compounds and that the root-associated microbiomes possess a diverse repertoire of phosphorus turnover mechanisms. We also showed that the microbiomes of both plant species encompass novel populations capable of mobilizing nitrogen and that the substrate strongly influences the dynamics of this cycle. Our results show that the interplay between plants and their microbiomes shapes nutrient turnover in the campos rupestres. We highlight that investigation of microbial diversity is fundamental to understand plant fitness in stressful environments.

The contribution of instream wood to streambed organic matter controls on microbial metabolic activity

<p>Microbial metabolic activity (MMA) in streambeds drives greenhouse gas (GHG) production and nutrient turnover. Previous research has identified that the quantity and quality of organic matter (OM) are important controls on MMA. Instream wood may make a significant contribution to the total OM content of the streambed, especially in forested streams, but it has typically been ignored or explicitly omitted in previous research. By means of an incubation experiment, we investigate the impact of streambed wood on MMA, GHG production and nutrient turnover rates. By using three geologies (sandstone, chalk and limestone) and allowing temperatures to fluctuate with environmental conditions, we observe these impacts under a range of typical scenarios. These results could have implications for estimates of GHG emissions from streams and inform catchment management, for example the impacts of direct installation of instream wood in river restoration or the indirect input as a result of riparian planting.</p>

Summer drought conditions promote the dominant role of phytoplankton in riverine nutrient dynamics

<p>Large rivers play a relevant role in the nutrient turnover from land to ocean. Here, highly dynamic planktonic processes are more important compared to streams making it necessary to link the dynamics of nutrient turnover to control mechanisms of phytoplankton. We investigated the basic conditions leading to high phytoplankton biomass and corresponding nutrient dynamics in the eutrophic River Elbe (Germany). In a first step, we performed six Lagrangian samplings in the lower river part at different hydrological conditions. While nutrient concentration remained high at low algal densities in autumn and at moderate discharge in summer, high algal concentrations occurred at low discharge in summer. Under these conditions, concentrations of silica and nitrate decreased and rates of nitrate assimilation were high. Soluble reactive phosphorus was depleted and particulate phosphorus increased inversely. Rising molar C:P ratios of seston indicated a phosphorus limitation of phytoplankton. Global radiation combined with discharge had a strong predictive power to explain maximum chlorophyll concentration. In a second step, we estimated nutrient turnover exemplarily for N during the campaign with the lowest discharge. Mass balance calculations revealed a total nitrate uptake of 455 mg N m<sup>-2</sup>d<sup>-1</sup> which was clearly dominated by assimilatory phytoplankton uptake whereas denitrification and other benthic processes were only of minor importance. Phytoplankton density, which showed a sigmoidal longitudinal development, dominantly explained gross primary production, related assimilatory nutrient uptake and respiration. Chlorophyll a concentration and bacterial abundance affected the composition of dissolved organic matter and were positively related to a number of CHO and CHNO components with high H/C and low O/C ratios but negatively to several CHOS surfactants. In conclusion, nutrient uptake in the large river strongly depends on the growth conditions for phytoplankton, which are favored during summer drought conditions.</p>

The influence of land use on nitrogen and sulfur turnover: a microbial approach

Saline lakes are subject to numerous environmental impacts related to human activities. Pollution is one of the major threats to water bodies, since it produces the increase of nitrogen and sulfur contents, changing the chemical and biological conditions of the ecosystem. Microbially mediated redox processes exert a fundamental control on nutrient and contaminant turnover. Therefore, the aim of this study was to determine the influence of land use on the microbial communities responsible for N and S turnover in the lacustrine sediments from Pétrola Lake (SE Spain) disturbed by anthropogenic activities (agriculture, farming, mining, and wastewaters). To reach this goal, chemical and molecular tools (sequencing of 16S rDNA gene) were applied. The results showed the influence of land use on the chemistry and microbial community structure of the sediments from the saline lake. Compared to natural conditions, wastewater and mining showed the largest differences in terms of microbial structure as a result of salinity. These findings provide better understanding of how land use affects the water chemistry and the abundance of organisms responsible for nutrient turnover.

Leaf Damage by Phytophagous Beetles alters Terminalia catappa Green and Senesced Leaf Chemistry

Chemical traits of Terminalia catappa L. leaves were determined on the island of Guam to understand the changes caused by beetle leaf herbivory. Green leaf chemistry indicated nitrogen was the most limiting nutrient in the climate and soils of Guam. The changes in leaf chemistry following beetle damage were extensive. Senesced leaf chemistry indicated beetle damage decreased some traits that predict lower leaf litter quality, such as lignin, but also decreased some traits that predict higher leaf litter quality, such as nitrogen. The stoichiometric traits based on carbon:macronutrient and lignin:macronutrient generally predicted higher quality leaf litter following beetle herbivory. The beetles that produce this form of T. catappa leaf damage on Guam are non-native, and overall, the results indicate these pests will increase the rate of litter decomposition and nutrient turnover in habitats where T. catappa is prevalent.