Use of the proteomic tool MALDI-TOF MS in termite identification

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) has proved effective for the identification of many arthropods. A total of 432 termite specimens were collected in Mali, Cote d’Ivoire, Togo, Senegal, Switzerland and France. Morphologically, 22 species were identified, including Ancistrotermes cavithorax, Amitermes evuncifer, Cryptotermes brevis, Cubitermes orthognathus, Kalotermes flavicollis, Macrotermes bellicosus, Macrotermes herus, Macrotermes ivorensis, Macrotermes subhyalinus, Microcerotermes parvus, Microtermes sp., Odontotermes latericius, Procubitermes sjostedti, Promirotermes holmgreni, Reticulitermes grassei, Reticulitermes lucifugus, Reticulitermes santonensis, Trinervitermes geminatus, Trinervitermes occidentalis, Trinervitermes togoensis, Trinervitermes sp., Trinervitermes trinervoides and Trinervitermes trinervius. Analysis of MALDI-TOF MS spectra profiles from termites revealed that all were of high quality, with intra-species reproducibility and inter-species specificity. Blind testing of the spectra of 389 termites against our updated database with the spectra of 43 specimens of different termite species revealed that all were correctly identified with log score values (LSVs) ranging from 1.65 to 2.851, mean 2.290 ± 0.225, median 2.299, and 98.4% (383) had LSVs > 1.8. This study is the first on the use of MALDI-TOF for termite identification and shows its importance as a tool for arthropod taxonomy and reinforces the idea that MALDI-TOF MS is a promising tool in the field of entomology.

Termites (Blattodea Latreille 1810, Termitoidae Latreille 1802) of Abuko Nature Reserve, Nyambai Forest Park and Tanji Bird Reserve (The Gambia)

From 28 October to 5 November 2013, a termite study was undertaken in 3 protected sites in The Gambia (West Africa). The aim of the study is to investigate the diversity of termites in three protected areas in the western region of the country. Termite sampling is carried out in 100 m × 2 m transects that are replicated three (3) times in each site. A total of thirty-one (31) termite species, that belong to fungus growing (11), harvester (1), humuvorous (12) and xylophagous (7), were recorded. The following nineteen (19) species are new to The Gambia: Coptotermes intermedius, Astalotermes near quietus, Ancistrotermes cavithorax, Macrotermes bellicosus, Microtermes grassei, M. lepidus, M. subhyalinus, Odontotermes erraticus, O. pauperans, O. sudanensis, Basidentitermes sp., Euchilotermes tensus arcuata, Noditermes cristifrons, Amitermes evuncifer, Amitermes spinifer, Microcerotermes fuscotibialis, Microcerotermes near parvulus, Microcerotermes near solidus and Promirotermes holmgreni. Additional description and/or ecological information on Odontotermes erraticus, Cubitermes severus, Cubitermes n. proximatus, Euchilotermes tensus arcuata, Basidentitermes sp., and Noditermes cristifrons are given.

New species and new records of Laboulbeniales (Ascomycota) from Sierra Leone

Nine new species of Laboulbeniales are described. These are Camptomyces africanus, parasitic on Astenus sp. (Coleoptera, Staphylinidae); Corethromyces golaensis, parasitic on Afrobium sp. (Staphylinidae); Diaphoromyces minutissimus, parasitic on Clavilispinus sp. (Staphylinidae); Laboulbenia antemnalis, parasitic on Macrotermes bellicosus (Isoptera, Termitidae); Laboulbenia buccalis, parasitic on Amitermes evuncifer (Termitidae); Laboulbenia insolita and L. kingsleyae, both parasitic on Abacetus pubescens (Coleoptera, Carabidae); Laboulbenia penetrans, parasitic on Stereocerellus singularis (Diptera, Chloropidae); Rhachomyces carbonii, parasitic on Tracypum sp. (Staphylinidae). Fourty-one species are reported for the first time from Sierra Leone, 12 of which are new for the whole African continent and 15 are recorded for the first time after description. A new synonymy is established: Kainomyces hyalinus Terada 1978 = K. alutellae Thaxt. 1931.

Evaluation Des Attaques De Termites (Isoptera Brulle, 1832) Sur Quatre Essences Forestieres D’importance Economique Au Togo (Afrique De L’ouest)

Termites are one of the most abundant invertebrates in tropical ecosystems, where they play a major ecological role in litter decomposition. Despite their important role, termites can cause considerable damage to wood structures in most tropical countries. The aims of this study was to evaluate termite attack in four economically important species in Togo including Antiaris africana Engl (Moraceae), Pterocarpus erinaceus Poir (Fabaceae), Triplochiton scleroxylon Schum (Malvaceae) (all local species) and teak or Tectona grandis Linné (Lamiaceae). For the latter species, termites were infested on 83.49% of 630 feet examined. However, the rate and type attacks differ from one plantation to another. On the studied forest species, termites were collected on about 97% of Triplochiton scleroxylon feet. For this species, at least 31% of examined feet have had their wood attacked. For the other two species, Pterocarpus erinaceus and Antiaris africana respectively about 80% and 67% of examined feet were attacked by termites. Amitermes evuncifer, Ancistrotermes guineensis and Microtermes lepidus were species of termite often harvested from trees examined. However the last two species namely A. guineensis and M. lepidus are encountered on the bark of trees inside veneers unlike A. evuncifer harvested in the heart of the wood. Therefore, the latter species deserves special attention as likely to cause serious damage not only to these species but also other species not taken into account in this study. It is therefore necessary to consider adequate control methods against these species.

Damage by insect pests to the Djingarey Ber Mosque in Timbuktu: detection and control

The Djingarey Ber Mosque in Timbuktu (Mali) is one of the most significant earthen construction in West Africa. Originally constructed in 1327, it was included in 1988 on the World Heritage UNESCO List for its unique architecture and historical importance. During its restoration, recently undertaken by the Aga Khan Trust for Culture, the wooden parts of the roof and architraves showed clear signs of threatening insect presence. In order to identify the pests responsible of the damage, evaluate its extent and suggest a proper control strategy, a detailed survey was performed inside the Mosque complex and in its immediate surroundings. The entomological inspection, performed in the dry-cold season, allowed to detect signs of insect damage in most of the wooden elements, even in the recently replaced beams, but also in walls, pillars and the precious decorated panels. Damages in the wood elements could be attributed to <em>Amitermes evuncifer</em> Silvestri (Termitidae), <em>Bostrychoplites zycheli </em>Marseuli (Bostrichidae) and <em>Lyctus africanus </em>Lesne (Lyctidae), which were collected alive on site. Injures in the walls and decorated panels appeared to be performed by hymenopterans such as &ldquo;plasterer bees&rdquo; (Colletidae) and Sphecidae. From the evaluation of the type and extent of damage in relation to the architecture and materials used in its construction and decoration, the most serious pest and the worse threat for the mosque is represented by termites. Control and preventive measures, in the view of a sustainable, long-lasting integrated management are suggested.