THE INFLUENCE OF NANO-SILVER ON FORMATION OF MICROBIAL BIOFILMS IN CASES OF TRAUMATIC LESION OF THE AUXILIARY APPARATUS OF THE EYE

Pyoinflammatory complications remain an acute problem in the post-operative period of traumatic lesions of the auxiliary apparatus of the eye (AAE). Silver both in the ionic form and in composition of chemical compounds is highly toxic for microorganisms, and as a result, it shows bactericidal effect to many bacterial strains, including gram-negative microorganisms. The peculiarity of AgNPs is efficiency of influence on the wide array of microorganisms, significant anti-biofilm effect and absence of resistance reaction. The aim of the research. To study the influence of the colloidal nano silver on formation of biofilms by microorganisms discharged from the wounds of patients with traumatic lesions of the auxiliary apparatus of the eye. Materials and methods. During 2018-2019, we examined 60 patients with traumatic lesions of the auxiliary apparatus of the eye. For evaluation of the influence of colloid nano silver solution on the processes of formation of the biofilm, we selected microorganisms which were cultured most frequently (Staphylococcus aureus, Acinetobacter spp., Klebsiella ozenae) from the patients. Results. The obtained data suggest that colloid nano silver inhibits efficiently formation of biofilms at the early stages (initiation, the 0 day of incubation) of their formation by all the three microorganisms, and the degree of inhibition of the biofilm formation did not depend on the silver concentration. The effect of colloid silver in the concentrations used by us at later stages of biofilm formation (the 3rd and the 7th day) with respect to К. ozenae is less efficient – the growth of cell biomass was observed (p≤0.05), and it did not depend on the silver concentration. At the same time, the effect of the colloid nano silver on S. aureus and Acinetobacter spp. on the 3rd and the 7th days was more efficient than at the early stage (p≤0.05). Conclusions. Nanoparticles of colloid silver are an efficient means to combat biofilms, as well as to prevent their formation.

USE OF PREPARATIONS CONTAINING COLLOID SILVER IN POTATO DISEASE CONTROL

The paper presents the results of four-ear trials of preparations with silver nanoparticles Zerox,Zeromiks, and Zerebra Agro on varieties with varying degrees of resistance to the main potato diseases. The four-ear trials made it possible to conclude that preparations with silver nanoparticles were effective in protecting potatoes from rhizoctoniosis and alternariosis, comparable to the effectiveness of chemical treaters (Maxim and Celeste Top) and fungicides used in the treatment of vegetative plants (Abiga Peak and Ridomil Gold MC). The biological effectiveness of preparations with silver nanoparticles was lower than that of Abig Peak by an average of 49.8% against late blight on the unstable Ilyinsky variety. The biological effectiveness of Zerebra Agro was low (24.6%) in relation to late blight on the Sante variety in the epiphytotic year. It was high (65.7%) in the non-epiphytotic year, and it was not much worse than chemical fungicides (30.4 and 78.1%, respectively) in both the epiphytotic and non-epiphytotic years.In 2018, when the effectiveness of the preparations was high for all major potato diseases,the yield increase was significantly higher than the check. In the standard version, it exceeded the check by 26.4%, in the variant with Zerebra Agro – by 27.1%. No significant difference was found between the standard variant and Zerebra Agro.

ON THE ROLE OF COLLOID SILVER IN THE TREATMENT OF UPPER RESPIRATORY TRACT AND EAR DISORDERS

The high resistance of infectious agents to antibiotics that doctors currently face in therapy of acute and chronic diseases, especially in ENT-practice makes it possible for experts to search for alternative paths and to select drugs with a broad spectrum of action. These include colloid silver based drugs with proven microbicidal, virucidal and local protective and anti-inflammatory properties