Protocol of the Healthy Brain Study: An accessible resource for understanding the human brain and how it dynamically and individually operates in its bio-social context

The endeavor to understand the human brain has seen more progress in the last few decades than in the previous two millennia. Still, our understanding of how the human brain relates to behavior in the real world and how this link is modulated by biological, social, and environmental factors is limited. To address this, we designed the Healthy Brain Study (HBS), an interdisciplinary, longitudinal, cohort study based on multidimensional, dynamic assessments in both the laboratory and the real world. Here, we describe the rationale and design of the currently ongoing HBS. The HBS is examining a population-based sample of 1,000 healthy participants (age 30–39) who are thoroughly studied across an entire year. Data are collected through cognitive, affective, behavioral, and physiological testing, neuroimaging, bio-sampling, questionnaires, ecological momentary assessment, and real-world assessments using wearable devices. These data will become an accessible resource for the scientific community enabling the next step in understanding the human brain and how it dynamically and individually operates in its bio-social context. An access procedure to the collected data and bio-samples is in place and published on https://www.healthybrainstudy.nl/en/data-and-methods/access. Trail registration: https://www.trialregister.nl/trial/7955.

Cyberbullying, metacognition, and quality of life: preliminary findings from the Longitudinal Adolescent Brain Study (LABS)

Cyberbullying contributes to poor mental health outcomes and quality of life (QoL), and peer victimisation has been shown to be positively associated with both positive and negative metacognition. Whilst metacognitive beliefs are associated with pathological worry, obsessive–compulsive symptoms, and rumination in depression, research is yet to examine whether metacognitive beliefs influence negative outcomes, such as reduced QoL, associated with experiences of cyberbullying. This study examines whether cybervictimisation, cyberbullying and metacognition play predictive roles in QoL, and if metacognition mediates any association between cybervictimisation, cyberbullying and QoL over time. Participants in the Longitudinal Adolescent Brain Study (LABS), aged 12 years and in grade 7, who had completed up to four assessment time-points (T) were included in this analysis: T1: N = 65; T2: N = 61; T3: N = 56; T4: N = 44. Structural equation modeling revealed significant associations between cyberbullying, cybervictimisation, metacognitive beliefs, and QoL. However, mediation analysis showed that only cognitive confidence acted as a partial mediator between cybervictimisation and QoL. The results suggest those who were more frequently cybervictimised had reduced confidence in their memory, which resulted in lower QoL. In addition, uncontrollability/danger and superstition, punishment, and responsibility were all negatively associated with QoL, indicating that as these metacognitive beliefs increased, QoL decreased. Our findings highlight the detrimental impact that cybervictimisation experiences can have on QoL in young people over time, and that some forms of metacognitive beliefs can also impact QoL. These findings can inform educators and health professionals on the importance of metacognition in regard to QoL over time, particularly in those who experience cybervictimisation.

The neuroscience of groove: neural mechanisms marrying music and movement

Music has a long history of being associated with movement synchronization such as foot-tapping or dance. These behaviours are easier with some music compared to others, and the reasons for this are not well understood. Groove is a quality of music that compels synchronous movement in the listener, and certain acoustic and musical features have been identified that contribute to a sense of groove.Neurons have been found to entrain to the beat of music. Combining these two ideas, it is reasonable to predict that neural populations involved in movement (i.e. premotor areas) would entrain more to high-groove than to low-groove music. This dissertation explores some of the psychological, musical and acoustic aspects of music that contribute to neural entrainment in premotor areas of the brain. Study 1 investigates the effects of feelings of groove on pre-motor entrainment, using stimuli that have been rated on extent of groove in a previous study. Study 2 investigates the musical feature of syncopation – which has previously been found to be associated with sense of groove – on extent of premotor entrainment and behavioural synchronization ability. Study 3 investigates the effects of acoustic features that have been found to be related to groove and movement synchronization such as event density and percussiveness. The pattern of results across all studies suggests that the complexity of the rhythms in the stimulus determines the extent of beat entrainment. Feelings of groove, however, are better characterized by “beat complexity”, which depends on a) the extent to which the listener perceives the beat, and b) the extent to which other rhythmic elements of the music compete with the beat. A network of brain areas integral to the perception of groove is proposed, where activation of premotor areas enables music to drive motor output.

The neuroscience of groove: neural mechanisms marrying music and movement

Music has a long history of being associated with movement synchronization such as foot-tapping or dance. These behaviours are easier with some music compared to others, and the reasons for this are not well understood. Groove is a quality of music that compels synchronous movement in the listener, and certain acoustic and musical features have been identified that contribute to a sense of groove.Neurons have been found to entrain to the beat of music. Combining these two ideas, it is reasonable to predict that neural populations involved in movement (i.e. premotor areas) would entrain more to high-groove than to low-groove music. This dissertation explores some of the psychological, musical and acoustic aspects of music that contribute to neural entrainment in premotor areas of the brain. Study 1 investigates the effects of feelings of groove on pre-motor entrainment, using stimuli that have been rated on extent of groove in a previous study. Study 2 investigates the musical feature of syncopation – which has previously been found to be associated with sense of groove – on extent of premotor entrainment and behavioural synchronization ability. Study 3 investigates the effects of acoustic features that have been found to be related to groove and movement synchronization such as event density and percussiveness. The pattern of results across all studies suggests that the complexity of the rhythms in the stimulus determines the extent of beat entrainment. Feelings of groove, however, are better characterized by “beat complexity”, which depends on a) the extent to which the listener perceives the beat, and b) the extent to which other rhythmic elements of the music compete with the beat. A network of brain areas integral to the perception of groove is proposed, where activation of premotor areas enables music to drive motor output.

Study Protocol: The Heart and Brain Study

BackgroundIt is well-established that what is good for the heart is good for the brain. Vascular factors such as hypertension, diabetes, and high cholesterol, and genetic factors such as the apolipoprotein E4 allele increase the risk of developing both cardiovascular disease and dementia. However, the mechanisms underlying the heart–brain association remain unclear. Recent evidence suggests that impairments in vascular phenotypes and cerebrovascular reactivity (CVR) may play an important role in cognitive decline. The Heart and Brain Study combines state-of-the-art vascular ultrasound, cerebrovascular magnetic resonance imaging (MRI) and cognitive testing in participants of the long-running Whitehall II Imaging cohort to examine these processes together. This paper describes the study protocol, data pre-processing and overarching objectives.Methods and DesignThe 775 participants of the Whitehall II Imaging cohort, aged 65 years or older in 2019, have received clinical and vascular risk assessments at 5-year-intervals since 1985, as well as a 3T brain MRI scan and neuropsychological tests between 2012 and 2016 (Whitehall II Wave MRI-1). Approximately 25% of this cohort are selected for the Heart and Brain Study, which involves a single testing session at the University of Oxford (Wave MRI-2). Between 2019 and 2023, participants will undergo ultrasound scans of the ascending aorta and common carotid arteries, measures of central and peripheral blood pressure, and 3T MRI scans to measure CVR in response to 5% carbon dioxide in air, vessel-selective cerebral blood flow (CBF), and cerebrovascular lesions. The structural and diffusion MRI scans and neuropsychological battery conducted at Wave MRI-1 will also be repeated. Using this extensive life-course data, the Heart and Brain Study will examine how 30-year trajectories of vascular risk throughout midlife (40–70 years) affect vascular phenotypes, cerebrovascular health, longitudinal brain atrophy and cognitive decline at older ages.DiscussionThe study will generate one of the most comprehensive datasets to examine the longitudinal determinants of the heart–brain association. It will evaluate novel physiological processes in order to describe the optimal window for managing vascular risk in order to delay cognitive decline. Ultimately, the Heart and Brain Study will inform strategies to identify at-risk individuals for targeted interventions to prevent or delay dementia.

Variable Anatomy of the Middle Cerebral Artery from Its Origin to the Edge of the Sylvian Fissure: A Direct Fresh Brain Study

The middle cerebral artery (MCA) is a major artery supplying blood to the brain and a common site of surgically treatable intracranial aneurysms. The MCA has anatomic variations that may have clinical significance. In order to investigate and document the extent of such variations, the MCA in 100 fresh brain hemispheres from 50 deceased patients, obtained from the Police Surgeon Office, Yangon General Hospital, Myanmar, was dissected and examined. Double MCA was observed in 2% of specimens. The termination patterns were bifurcation (72%), trifurcation (16%), and primary trunk (12%); early bifurcation was also observed (3%). The mean length of the main trunk (MT) was 20.6 ± 6.2 mm. The number of perforators ranged from 4 to 15 (mean = 9); most arose from the MT (96%), and the others originated at the bifurcation point (3%) and in postbifurcation divisions (1%). All of the perforators (100%) had a single branching pattern. The number of cortical branches ranged from 6 to 13 and included the orbitofrontal (98%), prefrontal (99%), precentral (95%), central (98%), temporopolar (87%), anterior temporal (89%), middle temporal (24%), posterior temporal (62%), temporo-occipital (69%), anterior parietal (88%), angular (83%), and posterior parietal (57%) arteries. Early cortical branches emerged from the MT in 52% of specimens. These data can help anatomists, radiologists, and neurosurgeons in preoperative assessment, surgical planning, and selection of surgical approach.

Rationale and design of the Healthy Brain Study: an accessible resource for understanding the human brain and how it dynamically and individually operates in its bio-social context

The endeavor to understand the human brain has seen more progress in the last few decades than in the previous two millennia. Still, our understanding of how the human brain relates to behavior in the real world and how this link is modulated by biological, social, and environmental factors is limited. To address this, we designed the Healthy Brain Study (HBS), an interdisciplinary, longitudinal, cohort study based on multidimensional, dynamic assessments in both the laboratory and the real world. Here, we describe the rationale and design of the currently ongoing HBS. The HBS is examining a population-based sample of 1,000 healthy participants (age 30-39) who are thoroughly studied across an entire year. Data are collected through cognitive, affective, behavioral, and physiological testing, neuroimaging, bio-sampling, questionnaires, ecological momentary assessment, and real-world assessments using wearable devices. These data will become an accessible resource for the scientific community enabling the next step in understanding the human brain and how it dynamically and individually operates in its bio-social context. An access procedure to the collected data and bio-samples is in place and published on https://www.healthybrainstudy.nl/en/data-and-methods.https://www.trialregister.nl/trial/7955