The influence of Metisevit on biochemical and morphological indicators of blood of piglets under nitrate loading

The article presents the results of research on the influence of the developed complex preparation Metisevit on the dynamics of morphological and biochemical blood indicators of piglets under nitrate loading. The research established that sodium nitrate intoxication causes disbalance of the physiological level of hematological indicators of the tested animals’ organisms. This was indicated by the manifestations of subclinical chronic nitrate-nitrite toxicosis: the increase in the level of nitrates, nitrites and methemoglobin in the blood. After prolonged feeding of the piglets with sodium nitrate at a dose of 0.3 g nitrate ion/kg, the concentration of nitrates and nitrites in the blood serum reached its maximum on the 60th day of the experiment. Also, the number of leukocytes and erythrocytes in the blood increased, and the activity of aspartate- and alanineaminotransferase in the blood serum increased. We rank the extent of liver intoxication with nitrates according to intensity of aminotransferase in the blood serum of the tested piglets. The normalization of morphological and biochemical indicators of blood of piglets under nitrate-nitrite intoxication requires usage of a preparation which contains vitamins, zeolites and antioxidants. If the fodder contains high doses of nitrates, 1.0 mg/kg dose of Metisevit is added to the fodder for preventing subclinical nitrate-nitrite toxicosis. Metisevit contains the following agents: phenozan acid, methionine, zeolite, selenium, vitamins E and C. The research conducted proved the feasibility of using Metisevit for preventing chronic nitrate-nitrite toxicosis in piglets. This preparation caused a decrease in the concentration of nitrates, nitrites and in the level of methemoglobin in the blood of piglets. Usage of Metisevit on piglets showed normalization of the number of erythrocytes and hemoglobin in the blood on the 10th day, and normalization of ASAT and ALAT on 30th and 90th days. The mechanism of Metisevit activity is connected with the direct contribution of its elements to the inhibition of the processes of absorbing metabolites which cause a toxic effect on the cell membranes of an animal’s organism. The absorbing activity of zeolite leads to decrease in concentration of agents which can be substrates for processes of peroxidation of lipids in the alimentary canal, and also to elimination of toxic pro-oxidant metabolites from the blood. This process occurs through osmosis and diffusion of these elements in the capillaries of microvilli of the small intestine and through their further fixation on sorbent granules.

Inhibition of thioacetamide induced liver intoxication and in-vitro antioxidant capacity of the Hugonia mystax

Background: The using of synthetic drugs in adequate manner for the treatments of liver diseases are causes the different side effect. Therefore, there is need to search for new bioactive compounds to control liver diseases. In this point of view, we selected the medicinal plant H. mystax to evaluate of its antioxidant and hepatoprotective potentiality and isolation of its constituents, because it has been using in traditional medicine in the treatment of different diseases.Methods: Different extracts were prepared from H. mystax using maceration extraction process.Antioxidant activity was carried out using standard test procedures on Superoxide, Hydroxyl and DPPH free radicals. Hepatoprotective was tested using Thioacetamide (TAA) induced liver intoxication in rats.Results: The H. mystax extracts were showed dose dependent antioxidant and hepatoprotective activities. Tested extracts of H. mystax have more activity on hydroxyl free radical than superoxide and DPPH radicals and among all extracts hydroalcoholic extract showed more activity. The extracts of H. mystax significantly restored the altered biochemical parameters due to TAA induced liver intoxication and hydroalcoholic extract exhibited more significant activity. So, the hydroalcoholic extract was used for the isolation of phytochemical constituents through column chromatography and isolated HM-1 and HM-2 compounds and are identified as stigmasterol and β-sitosterol.Conclusion: From the results of the study, it could be conclude that H. mystax have the capacity in reduction of free radicals and normalization of liver function when it damage due to different liver intoxications and further research is need to evaluate more pharmacological activities and in isolation of most active bioactive compounds from H. mystax.