Nine groups of knitted woolen fabrics for sportswear with different technical characteristics were treated with oxygen low-temperature plasma, and the changes in the surface morphology and chemical composition of wool fibers before and after plasma treatment, as well as the changes in the applicability indexes of knitted woolen fabrics, were studied. Finally, the comfort performance of the fabrics was evaluated by combining the concentration mapping method and the function evaluation value method. The analysis found that the surface scales of wool fibers were seriously etched after oxygen low-temperature plasma treatment, and the anti-felting, bursting strength and moisture absorption of the knitted woolen fabrics were improved. At the same time, the quick-drying index of the fabric has also been improved to a certain extent. This research provides a basis for the development of knitted woolen fabric for sportswear with excellent performance.