Development and Validation of Estimation of Genotoxic Impurity (Benzimidamidecontent) in Leflunomide by Using RP-HPLC Technique

The measurement of Genotoxic contaminant, a simple, selective, linear, accurate, and specific reverse phase high-performance liquid chromatographic (RP-HPLC) process was proposed. A Benzimidamide impurity in the medication Leflunomide has been discovered. Separation and analysis were carried out on Zorbax SB phenyl (4.6 mm x 250 mm) with a particle size of 5.0 μm. with 0.1 % Triethylamine in purified water with a pH of 7.0 and a buffer of phosphoric acid (20% in water). The mobile phase is a 40:60 mixture of buffer and Acetonitrile with degassing. Isocratic program mode was used. The elution was carried out at a rate of 1.0 mL/min with UV detection at a wavelength of 289 nm. The temperature of the selected column oven is 25°C. The linearity and accuracy of Benzimidamide are covered in this approach, with a LOQ limit of 150 percent (i.e.0.03 to 0.45 ppm). The observed correlation coefficient is 0.99994, with a range of 100.01 to 104.8 for recovery. The measured percent RSD of six spiked test preparation is below 5.0 percent in procedure precision (i.e. repeatability) and intermediate precision (IP). When maintained at room temperature, the standard and sample remained stable for three days. System appropriateness characteristics such as tailing factor and percent RSD do not exhibit significant changes in robustness experiments. For the detection of Benzimidamide the present RP-HPLC method is act as selective, robust, linear and precise.

Comparative studies of milk removal in lactating women using the Lactopuls breast pump with vacuum and compressive components and the Medela Symphony breast pump

Hypothesis/aims of study. To date, in the world, including in the Russian Federation, the number of women who use breast pumps (BPs) in the process of breastfeeding has significantly increased. Many mothers of premature and full-term babies are partially or completely breast pump-dependent for weeks or months and need a BP that should be effective and convenient. In this regard, it is of great practical interest to compare the characteristics and, above all, the efficiency of milk pumping using two types of BP one with vacuum component and another with vacuum and compressive components. The aim of this study was to conduct a comparative assessment of the efficiency of milk removal using the Medela Symphony BP and the Lactopuls BP. Study design, materials and methods. 14 lactating women of 1938 years of age who volunteered to be included in this study were examined. Women had been lactating and breastfeeding normally for 58 days. The Lactopuls BP with vacuum and compressive components and the Medela Symphony breast pump with a vacuum component were used in the main program mode. Results and conclusion. It was found that the Lactopuls BP is more effective for milk removal. In particular, the amount of milk expressed using it was on average 14% more than that using the Medela Symphony BP. The Laktopuls BP with vacuum and compression components has shown its higher efficacy compared to the vacuum Medela Symphony BP.