The environmental problems with each passing day has been gaining greater prominence in the news media of the whole world, but in a negative way. Water, an exhaustible natural resource, has been suffering enough through the misuse socioeconomic and environmental problems, pollution of resources water. With the population growth, the cities are receiving industrial facilities and this generates an increase of waste dumped, affecting the groundwater, and consequently affecting the quality of the water. With that, this study was carried out to evaluate the water conditions of beach Porto Real located in the river of Tocantins, in Porto Nacional/TO, for the purpose of bathing, accordance with the resolution CONAMA nº 274/00. The Coliliert method was used to obtain the values of faecal coliforms and the bacterium e-coli, the results showed low values, more than satisfactory, therefore, in the research period, Praia Porto Real waters presented satisfactory parameters in the bathing requirement.