A Comparison of Pregnancies That Occur Before and After the Onset of Chronic Fatigue Syndrome

Purpose. The aim of the present study was to elucidate the metabolic mechanisms associated with chronic fatigue syndrome (CFS) via an analysis of urine metabolites prior to and following exercise in a rat model. Methods. A rat model of CFS was established using restraint-stress, forced exercise, and crowded and noisy environments over a period of 4 weeks. Behavioral experiments were conducted in order to evaluate the model. Urine metabolites were analyzed via gas chromatography-mass spectrometry (GC-MS) in combination with multivariate statistical analysis before and after exercise. Results. A total of 20 metabolites were detected in CFS rats before and after exercise. Three metabolic pathways (TCA cycle; alanine, aspartate, and glutamate metabolism; steroid hormone biosynthesis) were significantly impacted before and after exercise, while sphingolipid metabolism alone exhibited significant alterations after exercise only. Conclusion. In addition to metabolic disturbances involving some energy substances, alterations in steroid hormone biosynthesis and sphingolipid metabolism were detected in CFS rats. Sphingosine and 21-hydroxypregnenolone may be key biomarkers of CFS, potentially offering evidence in support of immune dysfunction and hypothalamic-pituitary-adrenal (HPA) axis hypoactivity in patients with CFS.

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Differential heat shock protein responses to strenuous standardized exercise in chronic fatigue syndrome patients and matched healthy controls

Clinical & Investigative Medicine ◽

10.25011/cim.v31i6.4917 ◽

2008 ◽

Vol 31 (6) ◽

pp. 319 ◽

Cited By ~ 11

Author(s):

Anita A Thambirajah ◽

Kenna Sleigh ◽

H. Grant Stiver ◽

Anthony W Chow

Keyword(s):

Oxidative Stress ◽

Heat Shock ◽

Chronic Fatigue Syndrome ◽

Mononuclear Cells ◽

Chronic Fatigue ◽

Peripheral Blood Mononuclear ◽

Post Exercise ◽

Fatigue Syndrome ◽

Before And After ◽

Biologic Marker

Purpose: Since physical exertion is known to exacerbate the symptoms of chronic fatigue syndrome (CFS) and metabolic changes and including oxidative stress can modulate heat shock protein (HSP) expression responses, we sought to determine whether HSP expression is altered in CFS patients before and after exercise. Heat shock proteins (HSPs) in peripheral blood mononuclear cells (PBMC) were examined from 6 chronic fatigue syndrome (CFS) patients and 7 controls before and after a standardized treadmill exercise. Basal hsp27 was significantly higher among CFS patients compared to controls, and decreased immediately post-exercise, remaining below basal levels even at 7 days. A similar pattern was observed for HSP60, which gradually decreased in CFS patients but increased in controls post-exercise. These findings suggest an abnormal adaptive response to oxidative stress in CFS, and raise the possibility that HSP profiling may provide a more objective biologic marker for this illness. Methods: HSP27, HSP60, HSP70 and HSP90 expression from 6 CFS patients and 7 age- and sex-matched controls were examined by western blot analysis of peripheral blood mononuclear cells immediately before, after, and at 1 day and 7 days following a standardized treadmill exercise. Results: Basal HSP27 was higher among CFS patients than in controls (0.54 ± 0.13 vs. 0.19 ± 0.06, mean ± SEM; P < 0.01). In addition, these levels in CFS patients decreased immediately post-exercise (0.25 ± 0.09; P < 0.05) and remained below basal levels at day 1 post-exercises (0.18 ± 0.05; P < 0.05). P < 0.05). This declining expression of HSP27 during the post-exercise period among CFS patients was confirmed by one-way ANOVA analysis with repeated measures (P < 0.05). In contrast, HSP27 levels remained relatively constant following exercise among control subjects. Similar patterns of declining HSP levels in CFS patients were also observed for HSP60 (0.94 ± 0.40 vs. 1.32 ± 0.46; P < 0.05), and for HSP90 (0.34 ± 0.09 vs. 0.49 ± 0.10; P < 0.05) at day 7 post-exercise compared with basal levels, respectively. In contrast, HSP60 levels in control subjects increased at day 1 (1.09 ± 0.27) and day 7 (1.24 ± 0.50) post-exercise compared to corresponding levels immediately post-exercise (0.55 ± 0.06) (P < 0.05, respectively). Conclusion: These preliminary findings suggest an abnormal or defective adaptive response to oxidative stress in CFS, and raise the possibility that HSP profiling may provide a more objective biologic marker for this illness.

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Chronic fatigue syndrome and epigenetics: The case for hyperbaric oxygen therapy in biomarker identification

Journal of Pulmonology and Respiratory Research ◽

10.29328/journal.jprr.1001020 ◽

2021 ◽

Vol 5 (1) ◽

pp. 027-030

Author(s):

Shah Rajit J

Keyword(s):

Chronic Fatigue Syndrome ◽

Hyperbaric Oxygen ◽

Treatment Option ◽

Epigenetic Regulation ◽

Hyperbaric Oxygen Therapy ◽

Oxygen Therapy ◽

Chronic Fatigue ◽

Hypoxic Conditions ◽

Fatigue Syndrome ◽

Before And After

Chronic fatigue syndrome (CFS) is a poorly-understood respiratory condition that affects millions of individuals. Hyperbaric oxygen therapy (HBOT) is a treatment option being considered to address CFS as it is suggested to combat fatigue and increase oxygenation. HBOT provides two opportunities in advancing research of CFS: it may provide data on symptom amelioration and be utilized in the search for a biomarker. By either identifying biomarkers before using HBOT to compare epigenomes of patients before and after treatment or using HBOT to find epigenetic discrepancies between patients with and without treatment, matching epigenetic regulation with symptom amelioration may significantly advance the understanding of the etiology and treatment mechanism for CFS. EPAS1/HIF-2α is a leading candidate for an epigenetic biomarker as it responds differentially to hypoxic and normoxic conditions, which degrades more slowly in hypoxic conditions. Epigenetic regulation of EPAS1/HIF-2α in such differential conditions may be explored in HBOT experiments. In addition to HBOT as a promising treatment option for CFS symptoms, it may aid the identification of biomarkers in CFS. Further research into both outcomes is strongly encouraged.

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Differential Effects of Exercise on fMRI of the Midbrain Ascending Arousal Network Nuclei in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) and Gulf War Illness (GWI) in a Model of Postexertional Malaise (PEM)

Brain Sciences ◽

10.3390/brainsci12010078 ◽

2022 ◽

Vol 12 (1) ◽

pp. 78

Author(s):

James N. Baraniuk ◽

Alison Amar ◽

Haris Pepermitwala ◽

Stuart D. Washington

Keyword(s):

Chronic Fatigue Syndrome ◽

Threat Assessment ◽

Chronic Fatigue ◽

Gulf War ◽

Myalgic Encephalomyelitis ◽

Seed Region ◽

Gulf War Illness ◽

Fatigue Syndrome ◽

Before And After ◽

And Control

Background: Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS), Gulf War Illness (GWI) and control subjects underwent fMRI during difficult cognitive tests performed before and after submaximal exercise provocation (Washington 2020). Exercise caused increased activation in ME/CFS but decreased activation for GWI in the dorsal midbrain, left Rolandic operculum and right middle insula. Midbrain and isthmus nuclei participate in threat assessment, attention, cognition, mood, pain, sleep, and autonomic dysfunction. Methods: Activated midbrain nuclei were inferred by a re-analysis of data from 31 control, 36 ME/CFS and 78 GWI subjects using a seed region approach and the Harvard Ascending Arousal Network. Results: Before exercise, control and GWI subjects showed greater activation during cognition than ME/CFS in the left pedunculotegmental nucleus. Post exercise, ME/CFS subjects showed greater activation than GWI ones for midline periaqueductal gray, dorsal and median raphe, and right midbrain reticular formation, parabrachial complex and locus coeruleus. The change between days (delta) was positive for ME/CFS but negative for GWI, indicating reciprocal patterns of activation. The controls had no changes. Conclusions: Exercise caused the opposite effects with increased activation in ME/CFS but decreased activation in GWI, indicating different pathophysiological responses to exertion and mechanisms of disease. Midbrain and isthmus nuclei contribute to postexertional malaise in ME/CFS and GWI.

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Changes in the Allostatic Response to Whole-Body Cryotherapy and Static-Stretching Exercises in Chronic Fatigue Syndrome Patients vs. Healthy Individuals

Journal of Clinical Medicine ◽

10.3390/jcm10132795 ◽

2021 ◽

Vol 10 (13) ◽

pp. 2795

Author(s):

Sławomir Kujawski ◽

Anna M. Bach ◽

Joanna Słomko ◽

Derek F. H. Pheby ◽

Modra Murovska ◽

...

Keyword(s):

Chronic Fatigue Syndrome ◽

Chronic Fatigue ◽

Whole Body ◽

Control Group ◽

Static Stretching ◽

Healthy Individuals ◽

Baroreceptor Function ◽

Fatigue Syndrome ◽

Before And After ◽

Whole Body Cryotherapy

This study represents a comparison of the functional interrelation of fatigue and cognitive, cardiovascular and autonomic nervous systems in a group of Chronic Fatigue Syndrome (CFS) patients compared with those in healthy individuals at different stages of analysis: at baseline and after changes induced by whole-body cryotherapy (WBC) combined with a static-stretching (SS) program. The study included 32 patients (Fukuda criteria) and 18 healthy controls. Fatigue, cognitive, cardiovascular and autonomic function and arterial stiffness were measured before and after 10 sessions of WBC with SS. In the patients, a disturbance in homeostasis was observed. The network relationship based on differences before and after intervention showed comparatively higher stress and eccentricity in the CFS group: 50.9 ± 56.1 vs. 6.35 ± 8.72, p = 0.002, r = 0.28; and 4.8 ± 0.7 vs. 2.4 ± 1, p < 0.001, r = 0.46, respectively. Before and after intervention, in the CFS group increased fatigue was related to baroreceptor function, and baroreceptor function was in turn related to aortic stiffness, but no such relationships were observed in the control group. Differences in the network structure underlying the interrelation among the four measured criteria were observed in both groups, before the intervention and after ten sessions of whole cryotherapy with a static stretching exercise.

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Health care resource use by patients before and after a diagnosis of chronic fatigue syndrome (CFS/ME): a clinical practice research datalink study

BMC Family Practice ◽

10.1186/s12875-017-0635-z ◽

2017 ◽

Vol 18 (1) ◽

Cited By ~ 8

Author(s):

Simon M. Collin ◽

Inger J. Bakken ◽

Irwin Nazareth ◽

Esther Crawley ◽

Peter D. White

Keyword(s):

Health Care ◽

Clinical Practice ◽

Chronic Fatigue Syndrome ◽

Resource Use ◽

Chronic Fatigue ◽

Health Care Resource ◽

Practice Research ◽

Clinical Practice Research Datalink ◽

Fatigue Syndrome ◽

Before And After

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Physical activity before and after exercise in women with chronic fatigue syndrome

QJM ◽

10.1093/qjmed/91.7.465 ◽

1998 ◽

Vol 91 (7) ◽

pp. 465-473 ◽

Cited By ~ 29

Author(s):

S. Sisto

Keyword(s):

Physical Activity ◽

Chronic Fatigue Syndrome ◽

Chronic Fatigue ◽

Fatigue Syndrome ◽

Before And After

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Cardiovascular Responses of Women With Chronic Fatigue Syndrome to Stressful Cognitive Testing Before and After Strenuous Exercise

Psychosomatic Medicine ◽

10.1097/00006842-200109000-00009 ◽

2001 ◽

Vol 63 (5) ◽

pp. 756-764 ◽

Cited By ~ 19

Author(s):

John J. LaManca ◽

Arnold Peckerman ◽

Sue Ann Sisto ◽

John DeLuca ◽

Sean Cook ◽

...

Keyword(s):

Chronic Fatigue Syndrome ◽

Cardiovascular Responses ◽

Chronic Fatigue ◽

Cognitive Testing ◽

Strenuous Exercise ◽

Fatigue Syndrome ◽

Before And After

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Differential Effects of Exercise on fMRI of the Midbrain Ascending Arousal Network Nuclei in Myalgic Encephalomyelitis / Chronic Fatigue Syndrome (ME/CFS) and Gulf War Illness (GWI) in a Model of Postexertional Malaise (PEM)

10.20944/preprints202111.0420.v1 ◽

2021 ◽

Author(s):

James N Baraniuk ◽

Alison Amar ◽

Haris Pepermintwala ◽

Stuart D Washington

Keyword(s):

Chronic Fatigue Syndrome ◽

Threat Assessment ◽

Chronic Fatigue ◽

Gulf War ◽

Myalgic Encephalomyelitis ◽

Seed Region ◽

Fatigue Syndrome ◽

Before And After ◽

And Control ◽

Region Approach

Background: Myalgic Encephalomyelitis / Chronic Fatigue Syndrome (ME/CFS), Gulf War Ill-ness (GWI) and control subjects had fMRI during difficult cognitive tests performed before and after submaximal exercise provocation (Washington 2020). Exercise caused increased activation in ME/CFS but decreased activation for GWI in the dorsal midbrain, left Rolandic operculum and right middle insula. Midbrain and isthmus nuclei participate in threat assessment, attention, cognition, mood, pain, sleep, and autonomic dysfunction Methods: Activated midbrain nuclei were inferred by re-analysis of data from 31 control, 36 ME/CFS and 78 GWI subjects using a seed region approach and the Harvard Ascending Arousal Network. Results: Before exercise, control and GWI had greater activation during cognition than ME/CFS in left pedunculotegmental nucleus. Postexercise ME/CFS had greater activation than GWI for midline periaqueductal gray, dorsal and median raphe, and right midbrain reticular formation, parabrachial complex and locus coeruleus. The change between days (delta) was positive for ME/CFS but negative for GWI indicating reciprocal patterns of activation. Controls had no changes. Conclusions: Exercise caused opposite effects with increased activation in ME/CFS but decreased activation in GWI indicating different pathophysiological responses to exertion and mechanisms of disease. Midbrain and isthmus nuclei contribute to postexertional malaise in ME/CFS and GWI.

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