Assessment of Sperm Plasma Membrane Integrity and Viability

Studies were performed on boar semen routinely used at the local artificial insemination (AI) centre. The semen was stored in a Safe Cell Plus commercial extender at 17 °C for nine days. The aim of our research was focused on changes in sperm plasma membrane integrity. The integrity of the sperm plasma membrane and acrosome as well as sperm motility decreased after dilution and during storage of the semen. The highest percentage of live sperm was identified by the eosin-nigrosin method, a lower percentage by the SYBR-14/PI test, and the lowest percentage of live cells was discovered by the hypoosmotic swelling (HOS) test (P < 0.01). There were significant differences between the results of staining methods and sperm motility (P < 0.01). No significant differences were found between the HOS test results and sperm motility. The plasma membrane integrity parameters positively correlated (P < 0.001) with each other and with sperm motility but negatively with aspartate aminotransferase activity. Our findings confirmed that the boar sperm aging changes, which increased during liquid semen preservation, were connected with the loss of function and integrity of the sperm plasma membrane. The employed complementary tests are comprehensive indicators of sperm membrane integrity during long-term semen preservation, and they can help establish the actual number of ‘healthy’ cells. The assays may be used in AI laboratories and should be incorporated into the routine of semen analysis.

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Semen Quality of Garut Rams feed by Different Protein Sources and Their Implementation Potential in Small Farms of West Java

Jurnal Agripet ◽

10.17969/agripet.v20i1.15391 ◽

2020 ◽

Vol 20 (1) ◽

pp. 47-55

Author(s):

Kurnia Bagus Ariyanto ◽

Lilis Khotijah ◽

Dewi Apri Astuti ◽

Raden Iis Arifiantini ◽

Jean-Baptise Menassol

Keyword(s):

Plasma Membrane ◽

Semen Quality ◽

Membrane Integrity ◽

Black Soldier Fly ◽

Hermetia Illucens ◽

Brachiaria Humidicola ◽

Plasma Membrane Integrity ◽

Protein Sources ◽

Sperm Plasma Membrane

ABSTRACT. Maggot Hermetia illucens (Maggot Black Soldier Fly, MBSF) is an alternative protein source besides soybean meal (SBM) which may be used as a feed for improving the quality of semen particularly in Garut rams to support prolific nature. The aims of this study were to analyzed and compare the impact of different protein sources in feed on semen quality of Garut rams, and to assess the prediction ability of Garut rams to serve ewe in small-scale breeders in West Java, Indonesia. This study was conducted using a completely randomized design with 3 treatments and 4 replications, consisted of Brachiaria humidicola (BH) grass and T1 (concentrate contains 20% of SBM), T2 (concentrate contains 10% of SBM and 10% of MBSF), and T3 (concentrate contains 20% of MBSF). The parameters measured were feed consumption, semen quality (macroscopic and microscopic characteristics), also a potential ability of rams to serve ewe. The results showed there were no significant effect on protein consumption, semen volume, semen pH, semen color and consistency, sperm mass movement, sperm motility, sperm concentration, sperm morphology, and prediction potential ability to serve ewe. However, the result showed a significant effect (P0.05) on sperm viability and sperm plasma membrane integrity. Sperm plasma membrane integrity of ram feed with T3 was better than T1 and T2 (P0.05). The prediction potential ability rams to serve ewes on MBSF treatment was 38 heads, while in T1 and T2 were 43 and 57 heads, respectively. In conclusion, MBSF can be an alternative source of protein besides SBM to improve the semen quality of Garut rams. ABSTRAK. Maggot Hermetia illucens (Maggot Black Soldier Fly; MBSF) adalah sumber protein alternatif selain bungkil kedelai (SBM) yang dapat dipergunakan sebagai pakan untuk memperbaiki kualitas semen terutama pada domba Garut untuk mendukung sifat prolifik. Tujuan penelitian ini adalah menganalisis dan membandingkan dampak pemberian sumber protein berbeda terhadap kualitas semen domba Garut dan untuk menilai kemampuan domba Garut pejantan dalam melayani betina pada peternakan rakyat di Jawa Barat, Indonesia. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan 4 ulangan yang terdiri dari rumput Brachiaria humidicola (BH) dan T1 (konsentrat mengandung 20% SBM), T2 (konsentrat mengandung 10% SBM dan 10% MBSF), dan T3 (konsentrat mengandung 20% MBSF). Parameter yang diukur adalah konsumsi pakan, karakteristik semen (makroskopis dan mikroskopis) serta potensi domba jantan melayani betina. Hasil Penelitian menunjukkan tidak ada perbedaan signifikan pada konsumsi protein pakan, volume semen, pH semen, warna dan konsistensi semen, gerakan massa sperma, motilitas sperma, konsentrasi sperma, morfologi sperma, dan prediksi potensi pejantan dalam melayani betina. Namun, hasil penelitian menunjukkan terdapat perbedaan (P0.05) pada viabilitas sperma dan membran plasma utuh sperma. Membran plasma utuh pada perlakuan T3 lebih baik dibandingkan perlakuan T1 dan T2 (P0.05). Prediksi potensi betina terlayani dari pejantan yang diberi pakan MBSF adalah 38 ekor, sedangkan yang diberi SBM dan kombinasinya adalah 43 dan 57 ekor. Kesimpulan penelitian ini adalah MBSF dapat menjadi alternatif sumber protein selain bungkil kedelai dalam memperbaiki kualitas sperma domba Garut.

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Administration of Flutamide Alters Sperm Ultrastructure, Sperm Plasma Membrane Integrity and its Stability, and Sperm Mitochondrial Oxidative Capability in the Boar: In Vivo and In Vitro Approach

Reproduction in Domestic Animals ◽

10.1111/j.1439-0531.2011.01935.x ◽

2011 ◽

Vol 47 (4) ◽

pp. 635-643 ◽

Cited By ~ 7

Author(s):

M Lydka ◽

M Piasecka ◽

D Gaczarzewicz ◽

M Koziorowski ◽

B Bilinska

Keyword(s):

Plasma Membrane ◽

Membrane Integrity ◽

Sperm Ultrastructure ◽

Plasma Membrane Integrity ◽

Sperm Plasma Membrane

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Sperm plasma membrane integrity measurement: a combined method

Andrologia ◽

10.1111/j.1439-0272.1992.tb02665.x ◽

2009 ◽

Vol 24 (6) ◽

pp. 335-340 ◽

Cited By ~ 8

Author(s):

A. Carreras ◽

J. P. Ramirez ◽

C. Mendoza

Keyword(s):

Plasma Membrane ◽

Membrane Integrity ◽

Combined Method ◽

Plasma Membrane Integrity ◽

Sperm Plasma Membrane

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The effect of carob (Ceratonia siliqua) bean extract on male New Zealand White rabbit semen

World Rabbit Science ◽

10.4995/wrs.2018.10154 ◽

2018 ◽

Vol 26 (3) ◽

pp. 209 ◽

Cited By ~ 2

Author(s):

A. Ata ◽

O. Yildiz-Gulay ◽

S. Güngör ◽

A. Balic ◽

M.S. Gulay

Keyword(s):

Plasma Membrane ◽

New Zealand ◽

Plasma Concentration ◽

Seminal Plasma ◽

Sperm Concentration ◽

Membrane Integrity ◽

Ceratonia Siliqua ◽

Plasma Membrane Integrity ◽

Protein Levels ◽

Sperm Plasma Membrane

<p>The carob tree (Ceratonia siliqua) grows naturally in the Mediterranean region. The empiric use of carob cures for their aphrodisiac properties is very common in Turkey. Thus, the experiment was conducted to determine the effects of carob bean extracts on some reproductive parameters in male New Zealand White rabbits. During the adaptation period (stage 1), 6-8 mo old rabbits were trained in semen collection for 30 d. At the beginning of the treatment period (stage 2), rabbits were assigned randomly to 2 groups of 8 animals each. For a period of 49 d (1 spermatogenesis duration), one group was treated with a daily oral dose (10 mL) of carob extract and the other group received the corresponding volume of tap water. Semen was collected weekly. Semen samples taken at week 1 and 7 were analysed separately. At the beginning of stage 2, no differences were observed in the volume and pH of the ejaculate, sperm concentration, percentage of motility, percentage of live spermatozoa, percentage of sperm plasma membrane integrity, plasma concentration of testosterone, and seminal plasma protein levels between the control and carob extract treated animals. Similarly, at the end of stage 2, there were no differences in the volume and pH of the ejaculate, motility percentage, the percentage of live spermatozoa, percentage of sperm plasma membrane integrity, and the seminal plasma protein levels between the control and the carob extract treated animals. However, sperm concentration (P<0.05), plasma concentration of testosterone (P<0.05), and percentage of change in spermatozoa concentration (P<0.02) between groups were affected at the end of stage 2. The data suggested that the use of carob cures prepared by boiling carob fruit could have beneficial influences on sperm concentration in rabbits.</p>

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Extender supplementation with catalase maintains the integrity of sperm plasma membrane after freezing–thawing of semen from capuchin monkey

Zygote ◽

10.1017/s0967199416000447 ◽

2017 ◽

Vol 25 (2) ◽

pp. 231-234 ◽

Cited By ~ 1

Author(s):

Danuza L. Leão ◽

Adriel B. Brito ◽

Stefânia A. Miranda ◽

Karol G. Oliveira ◽

Débora V.C. Almeida ◽

...

Keyword(s):

Plasma Membrane ◽

Sperm Motility ◽

Membrane Integrity ◽

Capuchin Monkey ◽

Plasma Membrane Integrity ◽

Sapajus Apella ◽

Sperm Plasma Membrane ◽

Cryopreserved Sperm

SummaryWe aimed to evaluate the effect of supplementation of ACP-118® extender with the antioxidant catalase (10 and 50 µg/ml) on Sapajus apella sperm motility, vigour, and plasma membrane integrity during the processes of seminal liquefaction, cooling, and freezing. Catalase did not affect any of the evaluated parameters after semen dilution or cooling. Cryopreserved sperm in the presence of 50 µg/ml catalase presented a plasma membrane integrity similar to that fresh sperm, however.

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