Immunoperoxidase technique

Bone marrow mesenchymal stem cells (BM-MSCs) are used to repair hypoxic or ischemic tissue. After hypoxic the level of ATP is decreases, cellular functions do not continue and apoptosis or necrosis occur. Apoptosis is a progress of programmed cell death that occurs in normal or pathological conditions. In this study, we were investigated the hypoxic effect on apoptosis in mesenchymal stem cell. Bone marrow-derived stem cells were cultured in hypoxic (1% or 3%) or normoxic conditions 24, 96 well plates for 36 h. Cell viability was shown by MTT assay on 36 h. After fixation of cells with 4% paraformaldehyde, distributions of caspase-3, Bcl-2 and Bax with indirect immunoperoxidase technique, apoptotic cells with TUNEL assay were investigated. All staining results were evaluated using H-score analyses method with ANOVA, statistically. As a result, hypoxic condition was toxic for human mesenchymal stem cells and the number of death cell was higher in that than normoxic condition.

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Localisation of factor XIII in human tissues using an immunoperoxidase technique.

Journal of Clinical Pathology ◽

10.1136/jcp.37.5.560 ◽

1984 ◽

Vol 37 (5) ◽

pp. 560-563 ◽

Cited By ~ 54

Author(s):

J D Fear ◽

P Jackson ◽

C Gray ◽

K J Miloszewski ◽

M S Losowsky

Keyword(s):

Factor Xiii ◽

Immunoperoxidase Technique ◽

Human Tissues

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The distribution of neoplastic and normal B-lymphoid cells in nodular lymphomasUse of an immunoperoxidase technique on frozen sections

Human Pathology ◽

10.1016/s0046-8177(82)80002-6 ◽

1982 ◽

Vol 13 (7) ◽

pp. 610-617 ◽

Cited By ~ 34

Author(s):

N HARRIS ◽

R DATA

Keyword(s):

Lymphoid Cells ◽

Immunoperoxidase Technique ◽

Frozen Sections ◽

B Lymphoid

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Application of the immunoperoxidase technique FDR the detection of Derzsy's disease virus antigen in cell culture and goslings

Avian Pathology ◽

10.1080/03079458208436132 ◽

1982 ◽

Vol 11 (4) ◽

pp. 571-578 ◽

Cited By ~ 8

Author(s):

J. Roszkowski ◽

P. Gazdziński ◽

W. Kozaczyński ◽

M. Bartoszcze

Keyword(s):

Cell Culture ◽

Disease Virus ◽

Virus Antigen ◽

Immunoperoxidase Technique

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Dual fluorescence combined with a two-color immunoperoxidase technique: A new way of visualizing diverse neuronal elements

Journal of Neuroscience Methods ◽

10.1016/0165-0270(91)90044-z ◽

1991 ◽

Vol 36 (2-3) ◽

pp. 185-193 ◽

Cited By ~ 15

Author(s):

Paul M. Pilowsky ◽

Janusz Lipski ◽

Ross Prestidge ◽

Chun Jiang

Keyword(s):

Immunoperoxidase Technique ◽

Dual Fluorescence

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Distribution of lamprey gonadotropin-releasing hormone in the brain and pituitary gland of larval, metamorphic, and adult sea lampreys, Petromyzon marinus

Canadian Journal of Zoology ◽

10.1139/z94-008 ◽

1994 ◽

Vol 72 (1) ◽

pp. 48-53 ◽

Cited By ~ 26

Author(s):

Glenda M. Wright ◽

Kim M. McBurney ◽

John H. Youson ◽

Stacia A. Sower

Keyword(s):

Pituitary Gland ◽

Preoptic Area ◽

Petromyzon Marinus ◽

Gonadotropin Releasing Hormone ◽

Immunoperoxidase Technique ◽

Dorsal Region ◽

Releasing Hormone ◽

Sea Lampreys ◽

The Brain

Lamprey gonadotropin-releasing hormone was demonstrated in the brains of larval, metamorphic, and adult sea lampreys, Petromyzon marinus, using an immunoperoxidase technique. Gonadotropin-releasing hormone was observed in the neurohypophysis and preoptic area of the brain of larval, metamorphic, juvenile, and prespawning adults. The occurrence of immunoreactive cells and the intensity of the immunostaining was lowest in larvae, but by stage 5 of metamorphosis there was a marked increase in the prevalence and staining of these cells, which continued into adults. In larvae and lampreys in metamorphic stages 1–4, most immunoreactive fibres were confined to the dorsal region of the neurohypophysis. During stage 5 there was an expansion of immunopositive fibres into the ventral portion of the neurohypophysis. Prominent immunoreactivity was observed throughout the neurohypophysis from stage 5 onward through the adult stages. Changes in immunoreactivity of these cells and fibres in the brain and neurohypophysis correlate well with increased concentrations of hormone in the brain during development and with the timing of presumed changes in activity of cells in the adenohypophysis during metamorphosis.

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Synthesis of transforming growth factor-beta 1 by megakaryocytes and its localization to megakaryocyte and platelet alpha-granules

Blood ◽

10.1182/blood.v76.10.1946.bloodjournal76101946 ◽

1990 ◽

Vol 76 (10) ◽

pp. 1946-1955 ◽

Cited By ~ 1

Author(s):

RA Fava ◽

TT Casey ◽

J Wilcox ◽

RW Pelton ◽

HL Moses ◽

...

Keyword(s):

In Situ Hybridization ◽

Growth Factor ◽

Transforming Growth Factor Beta ◽

Messenger Rna ◽

Transforming Growth Factor ◽

Immunoperoxidase Technique ◽

Storage Site ◽

Tgf Beta ◽

Growth Factor Beta

We have directly demonstrated that megakaryocytes are a major site of synthesis and storage of transforming growth factor-beta 1 (TGF/beta 1) by combined immunohistochemical, immunocytochemical, and in situ hybridization methods. The presence of TGF/beta 1 messenger RNA (mRNA) in mature megakaryocytes in adult rat spleen and bone marrow (BM) was established by in situ hybridization. Localization of TGF/beta 1 protein to intact alpha-granules of megakaryocytes, its putative storage site, was accomplished in glycol-methacrylate embedded porcine BM with an immunoperoxidase technique and light microscopy. The TGF/beta 1 was sequestered in intracytoplasmic granules in a pattern virtually identical to that of another alpha-granule marker protein, fibrinogen. This observation strongly suggests packaging of TGF/beta 1 into this organelle within megakaryocytes. That TGF/beta 1 mRNA was localized to megakaryocytes suggests that the TGF/beta 1 found in the alpha-granules in platelets originates with megakaryocyte synthesis. The alpha-granule localization of TGF/beta 1, as well as fibrinogen, was also demonstrated in isolated platelets at the ultrastructural level by electronmicroscopy (EM) and postembedding colloidal-gold immunocytochemistry, thus directly demonstrating that alpha-granules are the final storage site for TGF/beta 1 in mature platelets.

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Varicella-zoster plaque assay and plaque reduction neutralization test by the immunoperoxidase technique

Journal of Clinical Microbiology ◽

10.1128/jcm.4.5.437-442.1976 ◽

1976 ◽

Vol 4 (5) ◽

pp. 437-442

Author(s):

G Gerna ◽

R W Chambers

Keyword(s):

Neutralization Test ◽

Plaque Assay ◽

Neutralizing Antibody ◽

Immunoperoxidase Technique ◽

Serum Samples ◽

Plaque Reduction Neutralization Test ◽

Varicella Zoster ◽

Convalescent Phase ◽

Antibody Titers ◽

Indirect Immunoperoxidase

A new plaque assay for the quantitation of varicella-zoster virus and a plaque reduction neutralization test for the determination of neutralizing antibody titer have been developed using the indirect immunoperoxidase technique. As compared with the classical plaque assay using a solid overlay, the test gives earlier results since plaque counting can be performed on day 3 after the inoculation of cell cultures. In six patients with zoster infection, neutralizing antibody titers ranged from 1:20 to 1:40 before the onset of infection and reached high levels (1:320 to 1:5,120) during the convalescent phase of the disease. Complement-fixing (CF) titers were all negative (less than 1:8) in prezoster serum samples from the same patients and ranged from 1:128 to 1:2,048 in the convalescent-phase sera. In the two cases in which late serum samples were available, neutralizing antibody titers matched the preillness levels, whereas CF titers dropped to undetectable levels. Neither neutralizing nor CF antibody was detected in two sera from individuals with no history of varicella-zoster infection. No differences in virus titers or neutralizing antibody titers were observed between the immunoperoxidase and the classical plaque assays. The appropriate characterization of reagent specificity is required before routine application of the test.

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