scholarly journals Dosimetry verification of three‐dimensional printed polylactic acid template‐guided precision 125 I seed implantation for lung cancer using a desktop three‐dimensional printer

2021 ◽  
Vol 22 (10) ◽  
pp. 202-209
Author(s):  
Xiaoyan Han ◽  
Shu Fang ◽  
Rui Sheng ◽  
Yi Wang ◽  
Jinhua Zhou ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Polylactic Acid ◽  
Three Dimensional ◽  
Seed Implantation

scholarly journals Mimicking Tumor Hypoxia in Non-Small Cell Lung Cancer Employing Three-Dimensional In Vitro Models

Cells ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 141
Author(s):  
Iwona Ziółkowska-Suchanek
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Tumor Hypoxia ◽  
Three Dimensional ◽  
In Vitro Models ◽  
Small Cell ◽  
Small Cell Lung ◽  
Multicellular Tumor Spheroid

Hypoxia is the most common microenvironment feature of lung cancer tumors, which affects cancer progression, metastasis and metabolism. Oxygen induces both proteomic and genomic changes within tumor cells, which cause many alternations in the tumor microenvironment (TME). This review defines current knowledge in the field of tumor hypoxia in non-small cell lung cancer (NSCLC), including biology, biomarkers, in vitro and in vivo studies and also hypoxia imaging and detection. While classic two-dimensional (2D) in vitro research models reveal some hypoxia dependent manifestations, three-dimensional (3D) cell culture models more accurately replicate the hypoxic TME. In this study, a systematic review of the current NSCLC 3D models that have been able to mimic the hypoxic TME is presented. The multicellular tumor spheroid, organoids, scaffolds, microfluidic devices and 3D bioprinting currently being utilized in NSCLC hypoxia studies are reviewed. Additionally, the utilization of 3D in vitro models for exploring biological and therapeutic parameters in the future is described.

scholarly journals Supercritical Impregnation of PLA Filaments with Mango Leaf Extract to Manufacture Functionalized Biomedical Devices by 3D Printing

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2125
Author(s):  
José María Rosales ◽  
Cristina Cejudo ◽  
Lidia Verano ◽  
Lourdes Casas ◽  
Casimiro Mantell ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
3D Printing ◽  
Polylactic Acid ◽  
Leaf Extract ◽  
Three Dimensional ◽  
3D Printer ◽  
Pharmacological Properties ◽  
Bioactive Properties ◽  
Supercritical Impregnation ◽  
Mango Leaves

Polylactic Acid (PLA) filaments impregnated with ethanolic mango leaves extract (MLE) with pharmacological properties were obtained by supercritical impregnation. The effects of pressure, temperature and amount of extract on the response variables, i.e., swelling, extract loading and bioactivity of the PLA filaments, were determined. The analysis of the filaments biocapacities revealed that impregnated PLA filaments showed 11.07% antidenaturant capacity and 88.13% antioxidant activity, which after a 9-day incubation shifted to 30.10% and 9.90%, respectively. Subsequently, the same tests were conducted on printed samples. Before their incubation, the printed samples showed 79.09% antioxidant activity and no antidenaturant capacity was detected. However, after their incubation, the antioxidant activity went down to only 2.50%, while the antidenaturant capacity raised up to 23.50%. The persistence of the bioactive properties after printing opens the possibility of using the functionalized PLA filaments as the feed for a three-dimensional (3D) printer.

scholarly journals In Situ Vitrification of Lung Cancer Organoids on a Microwell Array

Micromachines ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 624
Author(s):  
Qiang Liu ◽  
Tian Zhao ◽  
Xianning Wang ◽  
Zhongyao Chen ◽  
Yawei Hu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Drug Sensitivity ◽  
Simple Procedure ◽  
Three Dimensional ◽  
Cancer Drug ◽  
Microwell Array ◽  
Sensitivity Tests ◽  
Anti Cancer

Three-dimensional cultured patient-derived cancer organoids (PDOs) represent a powerful tool for anti-cancer drug development due to their similarity to the in vivo tumor tissues. However, the culture and manipulation of PDOs is more difficult than 2D cultured cell lines due to the presence of the culture matrix and the 3D feature of the organoids. In our other study, we established a method for lung cancer organoid (LCO)-based drug sensitivity tests on the superhydrophobic microwell array chip (SMAR-chip). Here, we describe a novel in situ cryopreservation technology on the SMAR-chip to preserve the viability of the organoids for future drug sensitivity tests. We compared two cryopreservation approaches (slow freezing and vitrification) and demonstrated that vitrification performed better at preserving the viability of LCOs. Next, we developed a simple procedure for in situ cryopreservation and thawing of the LCOs on the SMAR-chip. We proved that the on-chip cryopreserved organoids can be recovered successfully and, more importantly, showing similar responses to anti-cancer drugs as the unfrozen controls. This in situ vitrification technology eliminated the harvesting and centrifugation steps in conventional cryopreservation, making the whole freeze–thaw process easier to perform and the preserved LCOs ready to be used for the subsequent drug sensitivity test.

scholarly journals Three Dimensional Computed Tomography Lung Modeling is Useful in Simulation and Navigation of Lung Cancer Surgery

2013 ◽  
Vol 19 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Norihiko Ikeda ◽  
Akinobu Yoshimura ◽  
Masaru Hagiwara ◽  
Soichi Akata ◽  
Hisashi Saji
Keyword(s):  
Computed Tomography ◽  
Lung Cancer ◽  
Three Dimensional ◽  
Lung Cancer Surgery ◽  
Cancer Surgery

scholarly journals Interferon Consensus Sequence-Binding Protein 8, a Tumor Suppressor, Suppresses Tumor Growth and Invasion of Non-Small Cell Lung Cancer by Interacting with the Wnt/β-Catenin Pathway

2018 ◽  
Vol 51 (2) ◽  
pp. 961-978 ◽  
Author(s):  
Lin Ye ◽  
Tingxiu Xiang ◽  
Jing Zhu ◽  
Dairong Li ◽  
Qin Shao ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Tumor Suppressor ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Consensus Sequence ◽  
Three Dimensional ◽  
Small Cell ◽  
Immunofluorescence Staining ◽  
Small Cell Lung

Background/Aims: Interferon consensus sequence-binding protein 8 (IRF8) belongs to a family of interferon (IFN) regulatory factors that modulates various important physiological processes including carcinogenesis. As reported by others and our group, IRF8 expression is silenced by DNA methylation in both human solid tumors and hematological malignancies. However, the role of IRF8 in lung carcinoma remains elusive. In this study, we determined IRF8 epigenetic regulation, biological functions, and the signaling pathway involved in non-small cell lung cancer (NSCLC). Methods: IRF8 expression were determined by Q- PCR. MSP and A+T determined promotor methylation. MTS, clonogenic, Transwell assay, Flow cytometry, three-dimensional culture and AO/EB stain verified cell function. In vivo tumorigenesis examed the in vivo effects. By Chip-QPCR, RT-PCR, Western blot and Immunofluorescence staining, the mechanisms were studied. Results: IRF8 was significantly downregulated in lung tumor tissues compared with adjacent non-cancerous tissues. Furthermore, methylation-specific PCR analyses revealed that IRF8 methylation in NSCLC was a common event, and demethylation reagent treatment proved that downregulation of IRF8 was due to its promoter CpG hypermethylation. Clinical data showed that the IRF8 methylation was associated with tumor stage, lymph node metastasis status, patient outcome, and tumor histology. Exogenous expression of IRF8 in the silenced or downregulated lung cancer cell lines A549 and H1299 at least partially restored the sensitivity of lung cancer cells to apoptosis, and arrested cells at the G0/G1 phase. Cell viability, clonogenicity, and cell migration and invasive abilities were strongly inhibited by restored expression of IRF8. A three-dimensional culture system demonstrated that IRF8 changed the cells to a more spherical phenotype. Moreover, ectopic expression of IRF8 enhanced NSCLC chemosensitivity to cisplatin. Furthermore, as verified by Chip-qPCR, immunofluorescence staining, and western blotting, IRF8 bound to the T-cell factor/lymphoid enhancer factor (TCF /LEF) promoter, thus repressing β-catenin nuclear translocation and its activation. IRF8 significantly disrupted the effects of Wnt agonist, bml284, further suggesting its involvement in the Wnt/β-catenin pathway. Conclusion: IRF8 acted as a tumor suppressor gene through the transcriptional repression of β-catenin-TCF/LEF in NSCLC. IRF8 methylation may serve as a potential biomarker in NSCLC prognosis.

Reduction of observer variation using matched CT-PET for lung cancer delineation: A three-dimensional analysis

2006 ◽  
Vol 64 (2) ◽  
pp. 435-448 ◽  
Author(s):  
Roel J.H.M. Steenbakkers ◽  
Joop C. Duppen ◽  
Isabelle Fitton ◽  
Kirsten E.I. Deurloo ◽  
Lambert J. Zijp ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Dimensional Analysis ◽  
Three Dimensional ◽  
Observer Variation
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