Ultra‐Rapid Method for Coating Electrochemical Sensors with Antifouling Conductive Nanomaterials Enables Highly Sensitive Multiplexed Detection in Whole Blood

Safety and quality are key issues for the food industry. Consequently, there is growing demand to preserve the food chain and products against substances toxic, harmful to human health, such as contaminants, allergens, toxins, or pathogens. For this reason, it is mandatory to develop highly sensitive, reliable, rapid, and cost-effective sensing systems/devices, such as electrochemical sensors/biosensors. Generally, conventional techniques are limited by long analyses, expensive and complex procedures, and skilled personnel. Therefore, developing performant electrochemical biosensors can significantly support the screening of food chains and products. Here, we report some of the recent developments in this area and analyze the contributions produced by electrochemical biosensors in food screening and their challenges.

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Highly sensitive real-time detection of intracellular oxidative stress and application in mycotoxin toxicity evaluation based on living single-cell electrochemical sensors

The Analyst ◽

10.1039/d0an02015j ◽

2021 ◽

Author(s):

Lu Gao ◽

Jiadi Sun ◽

Liping Wang ◽

Qigao Fan ◽

Gaowen Zhu ◽

...

Keyword(s):

Oxidative Stress ◽

Electrochemical Sensor ◽

Single Cell ◽

Real Time ◽

Electrochemical Sensors ◽

Living Cells ◽

Selective Detection ◽

Toxicity Evaluation ◽

Highly Sensitive ◽

Intracellular Oxidative Stress

Single-cell electrochemical sensor is used in the local selective detection of living cells because of its high spatial–temporal resolution and sensitivity, as well as its ability to obtain comprehensive cellular physiological states and processes.

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Multiplexed Detection of Sepsis Markers in Whole Blood using Nanocomposite Coated Electrochemical Sensors

10.1101/2020.11.03.20224683 ◽

2020 ◽

Author(s):

Uroš Zupančič ◽

Pawan Jolly ◽

Pedro Estrela ◽

Despina Moschou ◽

Donald E. Ingber

Keyword(s):

Electrochemical Detection ◽

Whole Blood ◽

Electrochemical Sensors ◽

Point Of Care ◽

Sample Volume ◽

Nanocomposite Coating ◽

Cross Reactivity ◽

Detection Methods ◽

Clinical Samples ◽

Undiluted Serum

ABSTRACTSepsis is a leading cause of mortality worldwide that is difficult to diagnose and manage because this requires simultaneous analysis of multiple biomarkers. Electrochemical detection methods could potentially provide a way to accurately quantify multiple sepsis biomarkers in a multiplexed manner as they have very low limits of detection and require minimal sensor instrumentation; however, affinity-based electrochemical sensors are usually hampered by biological fouling. Here we describe development of an electrochemical detection platform that enables detection of multiple sepsis biomarkers simultaneously by incorporating a recently developed nanocomposite coating composed of crosslinked bovine serum albumin containing a network of reduced graphene oxide nanoparticles that prevents biofouling. Using nanocomposite coated planar gold electrodes, we constructed a procalcitonin sensor and demonstrated sensitive PCT detection in undiluted serum and clinical samples, as well as excellent correlation with a conventional ELISA (adjusted r2 = 0.95). Sensors for two additional sepsis biomarkers — C-reactive protein and pathogen-associated molecular patterns — were developed on the same multiplexed platform and tested in whole blood. Due to the excellent antifouling properties of the nanocomposite coating, all three sensors exhibited specific responses within the clinically significant range without any cross-reactivity in the same channel with low sample volume. This platform enables sensitive simultaneous electrochemical detection of multiple analytes in human whole blood, which can be expanded further to any target analyte with an appropriate antibody pair or capturing probe, and thus, may offer a potentially valuable tool for development of clinical point-of-care diagnostics.GRAPHICAL ABSTRACT

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Fabrication of Highly Sensitive and Selective Electrochemical Sensors for Detection of Paracetamol by Using Piroxicam Stabilized Gold Nanoparticles

Journal of The Electrochemical Society ◽

10.1149/2.0811709jes ◽

2017 ◽

Vol 164 (9) ◽

pp. B427-B434 ◽

Cited By ~ 4

Author(s):

Syeda Sara Hassan ◽

Sallahuddin Panhwar ◽

Ayman Nafady ◽

Abdullah M. Al-Enizi ◽

Sirajuddin ◽

...

Keyword(s):

Gold Nanoparticles ◽

Electrochemical Sensors ◽

Highly Sensitive

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Magnetic-capture-based SERS detection of multiple serum microRNA biomarkers for cancer diagnosis

Analytical Methods ◽

10.1039/c8ay02423e ◽

2019 ◽

Vol 11 (6) ◽

pp. 783-793 ◽

Cited By ~ 4

Author(s):

Hao Zhang ◽

Chaopeng Fu ◽

Shutao Wu ◽

Youqing Shen ◽

Chunhui Zhou ◽

...

Keyword(s):

Cancer Diagnosis ◽

Multiplexed Detection ◽

Serum Microrna ◽

Magnetic Capture ◽

Highly Sensitive ◽

Sers Detection

A highly sensitive and reproducible magnetic-capture SERS assay was developed for multiplexed detection of cancer-related microRNAs in serum solution.

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Corrigendum to “A SERS nano-tag-based magnetic-separation strategy for highly sensitive immunoassay in unprocessed whole blood” [Talanta 198 (2019) 527–533]

Talanta ◽

10.1016/j.talanta.2020.121530 ◽

2020 ◽

Vol 220 ◽

pp. 121530

Author(s):

Peng Zhao ◽

Han-xia Li ◽

Da-wei Li ◽

Ya-jun Hou ◽

Leilei Mao ◽

...

Keyword(s):

Magnetic Separation ◽

Whole Blood ◽

Highly Sensitive ◽

Separation Strategy

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181-P: A rapid method to enrich specific lymphocyte populations (T cells, B cells, total lymphocytes) from whole blood

Human Immunology ◽

10.1016/j.humimm.2007.08.204 ◽

2007 ◽

Vol 68 (1) ◽

pp. S106

Author(s):

Karina L. McQueen ◽

Jenna L. Warren ◽

Allen C. Eaves ◽

Terry E. Thomas

Keyword(s):

T Cells ◽

B Cells ◽

Rapid Method ◽

Whole Blood ◽

Lymphocyte Populations

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Highly sensitive gas chromatographic analysis of ethanol in whole blood, serum, urine, and fecal supernatants by the direct injection method

Clinical Chemistry ◽

10.1093/clinchem/43.6.1003 ◽

1997 ◽

Vol 43 (6) ◽

pp. 1003-1009 ◽

Cited By ~ 34

Author(s):

Albert Tangerman

Keyword(s):

Blood Serum ◽

Whole Blood ◽

Direct Injection ◽

Glass Beads ◽

Injection Technique ◽

Large Sample ◽

Highly Sensitive ◽

Gas Chromatographic Method ◽

Ethanol Determination ◽

Gas Chromatographic Separation

Abstract A highly sensitive, reproducible, and rapid gas chromatographic method for ethanol determination in various biological specimens (human whole blood, serum, urine, and fecal supernatants) was developed. The method involves direct injection of the biological specimen into the gas chromatograph, without any pretreatment. Contamination of the gas chromatographic column with nonvolatile material was prevented by the use of a glass liner in the injector. This liner, which acted as a precolumn, was partly filled with small glass beads. Injection was performed in between the glass beads. More than 50 injections of the various biological specimens could be done before the liner had to be replaced by a new one. This injection technique between glass beads allows direct injection of large sample volumes up to 10 μL without disturbing the gas chromatographic separation. Injection of these large sample volumes made the method very sensitive. The detection limit for ethanol amounted to 0.1 mg/L (2 μmol/L) when using an injection volume of 5 μL. Attention has also been paid to simultaneously monitoring ethanol, methanol, acetaldehyde, and acetone in blood and urine of control subjects.

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