Random donor platelet crossmatching: Comparison of four platelet antibody detection methods

1988 ◽  
Vol 28 (1) ◽  
pp. 1-7 ◽  
Author(s):  
J. Freedman ◽  
M. B. Garvey ◽  
A. Hornstein ◽  
Z. Salomon De Friedberg ◽  
V. Blanchette
Keyword(s):  
Detection Methods ◽  
Antibody Detection ◽  
Platelet Antibody

Diagnosis of schistosomiasis: antibody detection, with notes on parasitological and antigen detection methods

Parasitology ◽  
1999 ◽  
Vol 117 (7) ◽  
pp. 41-57 ◽  
Author(s):  
J. V. HAMILTON ◽  
M. KLINKERT ◽  
M. J. DOENHOFF
Keyword(s):  
Recombinant Dna ◽  
Infection Intensity ◽  
Public Health Problem ◽  
Antigen Detection ◽  
Detection Methods ◽  
Antibody Detection ◽  
Native Form ◽  
Keyhole Limpet Haemocyanin ◽  
Advantages And Disadvantages ◽  
Wide Range

Schistosomiasis remains a serious world-wide public health problem with a still unfulfilled need for routine cost-effective methods of diagnosis. Such methods are required not only for people in endemic areas, but increasingly for tourists who may have become infected during visits to such places. This article reviews the wide range of immunoassays and antigenic preparations that have been shown to have potential for diagnosis of schistosomiasis by the indirect method of antibody detection. Antigens in native form derived from cercariae, adult worms and eggs are considered, as well as schistosome antigens produced by recombinant DNA technology and the schistosome cross-reactive antigen, keyhole limpet haemocyanin (KLH). Respective advantages and disadvantages of antibody detection, circulating antigen detection and parasitological methods of diagnosis are analysed. It is suggested that due to the relative insensitivity of both parasitology and antigen detection, antibody detection methods could find increasing use in situations of low infection intensity.

Antiglycolipid antibodies in Guillain-Barré and Fisher syndromes: discovery, current status and future perspective

2020 ◽  
pp. jnnp-2020-325053
Author(s):  
Susumu Kusunoki ◽  
Hugh J Willison ◽  
Bart C Jacobs
Keyword(s):  
Acute Phase ◽  
Axonal Neuropathy ◽  
Detection Methods ◽  
Antibody Detection ◽  
Current Status ◽  
Future Perspective ◽  
Acute Motor Axonal Neuropathy ◽  
Cns Diseases ◽  
Fisher Syndrome ◽  
Guillain Barré

Guillain-Barré syndrome (GBS) and Fisher syndrome (FS) are acute autoimmune neuropathies, often preceded by an infection. Antiglycolipid antibody titres are frequently elevated in sera from the acute-phase patients. Particularly, IgG anti-GQ1b antibodies are positive in as high as 90% of FS cases and thus useful for diagnosis. The development of animal models of antiglycolipid antibody-mediated neuropathies proved that some of these antibodies are directly involved in the pathogenetic mechanisms by binding to the regions where the respective target glycolipid is specifically localised. Discovery of the presence of the antibodies that specifically recognise a new conformational epitope formed by two different gangliosides (ganglioside complex) in the acute-phase sera of some patients with GBS suggested the carbohydrate–carbohydrate interaction between glycolipids. This finding indicated the need for further research in basic glycobiological science. Antiglycolipid antibodies, in particular antigangliosides antibodies, are mostly detected in acute motor axonal neuropathy type of GBS and in FS, and less frequently in the acute inflammatory demyelinating polyneuropathy (AIDP) type of GBS or in central nervous system (CNS) diseases. In the future, the search for the putative antibodies in AIDP and those that might be present in CNS diseases should continue. In addition, more efficient standardisation of antiglycolipid antibody detection methods and use as biomarkers in daily clinical practice in neurology is needed.

52-P: Correlations of donor-specific antibody detection methods

2009 ◽  
Vol 70 ◽  
pp. S37
Author(s):  
Younhee Park ◽  
Hyon-Suk Kim ◽  
Dong Il Won ◽  
Hae Jin Kim ◽  
Yu Seun Kim
Keyword(s):  
Specific Antibody ◽  
Detection Methods ◽  
Antibody Detection

Platelet antibody: Review of detection methods

1988 ◽  
Vol 29 (2) ◽  
pp. 106-114 ◽  
Author(s):  
Kenneth A. Schwartz
Keyword(s):  
Detection Methods ◽  
Platelet Antibody

C1q-Binding HLA Antibodies Do Not Predict Platelet Transfusion Failure in TRAP Study Participant

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 1562-1562
Author(s):  
Rachael P. Jackman ◽  
Douglas Bolgiano ◽  
Mila Lebedeva ◽  
Sherrill J. Slichter ◽  
Philip J. Norris
Keyword(s):  
Conflicts Of Interest ◽  
Fluorescent Antibody ◽  
Study Participant ◽  
Class I ◽  
Detection Methods ◽  
Antibody Detection ◽  
Hla Antibodies ◽  
Luminex Assay ◽  
Single Antigen ◽  
Antibody Levels

Abstract Introduction: In the Trial to Reduce Alloimmunization to Platelets (TRAP) study, 101 of the 530 subjects became clinically refractory (CR) to platelet transfusions in the absence of detectable antibodies against HLA as measured by the lymphocytotoxicity assay (LCA). Using more sensitive bead-based detection methods we have previously demonstrated that while many of these LCA- patients do have anti-HLA antibodies, that these low to moderate level antibodies do not predict refractoriness. In addition to being less sensitive then bead based methods, the LCA screen only detects complement-binding antibodies. As these antibodies could be important for platelet rejection, we assessed if previously undetected complement-binding antibodies could account for some of the refractoriness seen in LCA- patients. Methods: 169 LCA- (69 CR, 100 non-CR) and 20 LCA+ (10 CR, 10 non-CR) subjects were selected from the TRAP study. Anti-class I HLA IgG and C1q binding antibodies were measured in serum or plasma using two different multi-analyte, semi-quantitative, bead-based fluorescent antibody detection assays: the LabScreen mixed Luminex assay, and the LabScreen single antigen class I assay with or without added EDTA (One Lambda). Groups were compared using an unpaired t-test, a=0.05, and correlation between variables was also assessed. Results: New measurements of anti-class I HLA IgG antibodies reliably reproduced earlier data with a strong correlation between the old and new measurements (R2=0.9736, p<0.0001). While some of the LCA- subjects did have detectable C1q-binding anti-class I HLA antibodies, and some LCA+ subjects did not, levels of these antibodies were significantly higher among LCA+ subjects (p<0.0001). Levels of C1q-binding anti-class I HLA antibodies were not significantly different between CR and non-CR among either the LCA- or LCA+ subjects. Conclusions: While complement-binding anti-class I HLA antibody levels were higher in the LCA+ subjects, higher levels of these antibodies were not seen in CR LCA+ patients as compared with non-CR LCA+ patients. Complement-binding anti-class I HLA antibodies do not account for refractoriness seen among the LCA- TRAP subjects. This work confirms that low to mid level anti-class I antibodies do not drive refractoriness to platelets, and suggests that antibody-independent mechanisms cause refractoriness in patients lacking higher levels of anti-HLA antibodies. Disclosures No relevant conflicts of interest to declare.

scholarly journals Comparisons of Anti-dsDNA Antibody Detection Methods by Chemiluminescent Immunoassay and Enzyme-Linked Immunosorbent Assay in Systemic Lupus Erythematosus

Diagnostics ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1940
Author(s):  
Huang-Chen Chang ◽  
Yen-Ching Wu ◽  
Jun-Peng Chen ◽  
Yi-Da Wu ◽  
Wen-Nan Huang ◽  
...  
Keyword(s):  
Disease Activity ◽  
Immunosorbent Assay ◽  
Lupus Erythematosus ◽  
Predictive Power ◽  
Detection Methods ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dsdna Antibody ◽  
Chemiluminescent Immunoassay ◽  
Diagnostic Power

This study aimed to compare the test results of anti-double-stranded DNA (anti-dsDNA) antibodies obtained using chemiluminescent immunoassay (CIA) and enzyme-linked immunosorbent assay (ELISA), and investigate predictors of inconsistent results. This retrospective study included 502 patients who underwent CIA and ELISA to determine their anti-dsDNA antibody values within a year. We compared the diagnostic power for SLE, disease activity, and predictive power for lupus nephritis (LN). A multivariate analysis was performed to determine the predictors of inconsistencies. CIA and ELISA were moderately correlated in terms of their consistency (Cronbach’s α = 0.571), and yielded comparably favorable results in terms of SLE diagnostic power and SLE disease activity. However, if the patient had LN, CIA displayed higher predictive power than ELISA (0.620 vs. 0.555, p = 0.026). Compared with the CIA/ELISA double-positive group, the inconsistent group had lower anti-C1q circulating immune complexes (CIC) antibody values (OR: 0.42, 95% CI: 0.18–0.94, p = 0.036), and lower SLEDAI scores (≥4) (OR: 0.33, 95% CI: 0.14–0.79, p = 0.013). Anti-dsDNA antibody detection with CIA exhibited higher predictability for diagnosing LN than did ELISA. In the event of inconsistencies between anti-dsDNA methods, SLE disease activity and CIC test values should be considered simultaneously.

scholarly journals Feasibility Study of an Optical Caustic Plasmonic Light Scattering Sensor for Human Serum Anti-Dengue Protein E Antibody Detection

2016 ◽  
Author(s):  
A.A. Garcia ◽  
L.S. Franco ◽  
M.A. Pirez-Gomez ◽  
J.L. Pech-Pacheco ◽  
J.F. Mendez-Galvan ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Light Scattering ◽  
Human Serum ◽  
Feasibility Study ◽  
Mie Theory ◽  
Oblate Spheroid ◽  
Detection Methods ◽  
Antibody Detection ◽  
Dot Blot ◽  
Predictive Values

AbstractAntibody detection and accurate diagnosis of tropical diseases is essential to help prevent the spread of disease. However, most detection methods lack cost-effectiveness and field-portability, which are essential features for achieving diagnosis in a timely manner in developing countries. To address this problem, transparent 3D printed sample chambers with a total volume of 700 microliters and an oblate spheroid shape were fabricated to measure green light scattering of gold nanoparticles using an optical caustic focus to detect antibodies. Scattering signals from 90 degree scattering of 20, 40, 50, 60, 80, 100, and 200 nm gold nanoparticles using a green laser and standard quartz cuvette were compared to the scattering signals from a green LED light source with an oblate spheroid sample chamber and to Mie theory by fitting the data to a logistic curve. The change in signal from 60 nm to 120 nm decreased in the order of Mie Theory > Optical Caustic scattering > standard laser 90 degree scattering. These results suggested that conjugating 60 nm gold nanoparticles with Dengue Protein E and using an optical caustic system to detect plasmonic light scattering would result in a sensitive test for detecting human antibodies against Dengue Protein E in serum. To explore this possibility, we studied the light scattering response of protein E conjugated gold nanoparticles exposed to different concentrations of anti-protein E antibody, and posteriorly via a feasibility study consisting of 10 human serum samples using a modified dot blot protocol and a handheld optical caustic-based sensor device. The overall agreement between the benchtop light scattering and dot blot results and the handheld optical caustic sensor suggest that the new sensor concept shows promise to detect gold nanoparticle aggregation caused by the presence of the antibody using a homogeneous assay. Further testing and protocol optimization is needed in order to draw conclusions on the positive predictive and negative predictive values for this new testing system.

scholarly journals AET-treated platelets: their usefulness for platelet antibody detection and an examination of their altered sensitivity to immune lysis

Blood ◽  
1979 ◽  
Vol 54 (5) ◽  
pp. 1101-1108
Author(s):  
PL Cimo ◽  
SA Gerber
Keyword(s):  
Blood Cells ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Antibody Detection ◽  
Enhanced Sensitivity ◽  
Platelet Antibodies ◽  
Different Types ◽  
Drug Dependent ◽  
Altered Sensitivity ◽  
Bernard Soulier Syndrome ◽  
Platelet Antibody

2-Aminoethylisothiouronium bromide (AET) increases the sensitivity of blood cells to complement-mediated immune lysis. We compared the sensitivities of untreated or AET-treated platelets to immune lysis induced by different types of platelet antibody in the 51Cr platelet lysis test. AET platelets were 8–16 times more sensitive to autoantibody and alloantibody, but 8–16 times less sensitive to drug- dependent antibody. AET-platelets bound similar amounts of alloantibody but less drug-dependent antibody, and they lysed at higher complement dilutions than did untreated platelets. AET-platelets detected 10 of 25 autoantibodies, 9 of 9 alloantibodies, and 5 of 8 drug-dependent antibodies. Untreated platelets detected 1 of 25, 6 of 9, and 7 of 8 of these respective platelet antibodies. The use of AET-platelets in the 51Cr platelet lysis test increases its sensitivity for detecting non- drug-dependent platelet antibodies. AET-platelets resemble paroxysmal nocturnal hemoglobinuria (PNH) platelets in their enhanced sensitivity to complement-mediated lysis. They differ from PNH platelets in their insensitivity to immune lysis induced by drug-dependent antibodies and, in this respect, are similar to Bernard-Soulier syndrome platelets.

scholarly journals Clinical Value of Two Detection Methods for Mycoplasma Pneumoniae Antibody in the Diagnosis of Mycoplasma Pneumoniae Infection in North China

2021 ◽  
Author(s):  
Jupeng Wang ◽  
Lina Zhu ◽  
Mingkun Ma ◽  
Hui Chen ◽  
Suxiang Guo ◽  
...  
Keyword(s):  
Tract Infection ◽  
Respiratory Tract ◽  
Respiratory Tract Infection ◽  
Mycoplasma Pneumoniae ◽  
North China ◽  
Detection Methods ◽  
Antibody Detection ◽  
Positive Rate ◽  
Adults And Children ◽  
Males And Females

Abstract Background: To analyze the detection of Mycoplasma pneumoniae in adults and children in North China using two antibody detection methods, and to guide prevention and treatment.Methods: A retrospective study was conducted from September 2017 to May 2021. Mycoplasma pneumoniae total antibody was detected using particle agglutination (PA). Anti-Mycoplasma pneumoniae IgM in patients with respiratory tract infection was detected by indirect immunofluorescence. All patients were divided into 9 groups according to age: ≤1, 2-3, 4-6, 7-14, 15-18, 19-39, 40-59, 60-79, ≥80,Results: The positive rate of Mycoplasma pneumoniae total antibody in 5,666 patients with community-acquired pneumonia was 40.13%. In adults and children, the positive rates were 19.92% and 77.3% (p<0.05), respectively. The positive rates in males and females was 37.89% and 42.40% (p<0.05), respectively. The positive rate for anti-Mycoplasma pneumoniae IgM in 5,746 patients with respiratory tract infection was 28.56%, and 10.37% and 36.82% in adults and children (p<0.05), respectively. In males and females, the positive rate for anti-Mycoplasma pneumoniae IgM was 24.56% and 33.38% (p<0.05). The highest positive rates for total antibody and anti-Mycoplasma pneumoniae IgM were recorded in autumn. Of the 1,975 patients tested for Mycoplasma pneumoniae antibody using both methods simultaneously, 26.71% were negative for total antibody and 8.63% had titers ranging between 1:40 and 1:80 when positive for IgM antibody. When negative for anti-Mycoplasma pneumoniae IgM, total antibody titer was ≥1:160 in 34.94% of the patients.Conclusion: Mycoplasma pneumoniae is the main cause of respiratory tract infection and its incidence is highest in autumn. Because Mycoplasma pneumoniae was more commonly detected in women and children, screening should be strengthened in these groups.

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