Aims: Oncology treatments aim at selective toxicity for tumor (compared to normal) cells, and chromone-
coumarin hybrids have shown such activity.
Methods:
In this study, we test a novel series of synthetic chromone and coumarin derivatives (1–9) for cytotoxic
activity against a panel of tumor cell lines (MCF-7, A549, HepG2, HTC-116, B16 and Caco-2) opposed to
non-tumor cells (HEK-293t). Electrical impedance spectroscopy was used to monitor cell viability in real time.
Results:
Compound 8 showed the most potent activity, and it significantly diminished cancer cell proliferation
and viability in different cell lines. It induced apoptosis in a dose-dependent manner, as shown by Western blot
and flow cytometry.
Conclusion:
Electrical impedance spectroscopy appears to be a convenient tool for in vitro cytotoxicity analysis,
which could be useful for identifying drug effects and side effects during early phases of drug discovery and
development.
In vitro analysis of various cell lines responses to electroporative electric pulses by means of electrical impedance spectroscopy
