Comparative study of rapid ATP bioluminescence assay and conventional plate count method for development of rapid disinfecting activity test

Luminescence ◽  
2021 ◽  
Author(s):  
Masako Ishimaru ◽  
Hideyuki Noda ◽  
Erino Matsumoto ◽  
Hiroyuki Koshi ◽  
Hideyuki Otake
Keyword(s):  
Comparative Study ◽  
Plate Count ◽  
Activity Test ◽  
Count Method ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Plate Count Method ◽  
Conventional Plate ◽  
Atp Bioluminescence Assay

Study on the Detection of Total Colony in Medical and Health Environment Based on ATP Bioluminescence

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
Zhe Li
Keyword(s):  
Culture Method ◽  
Pass Rate ◽  
Plate Count ◽  
Count Method ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Plate Count Method ◽  
The Difference ◽  
Atp Bioluminescence Assay ◽  
Bioluminescence Method

In this paper, the application of ATP fluorescence in the detection of colonies in the health environment of hospitals was studied. Firstly, the principle of ATP bioluminescence method was described. Then, ATP bioluminescence and plate count method were used to test the density of the surface of the objects in selected area, taking the time points 2 hours after disinfection as the time nodes. The results showed that the difference between the qualified rate of ATP bioluminescence assay and the plate count method was statistically significant {P<0.01}. Therefore, ATP bioluminescence method was highly correlated with bacterial culture method. The correlation coefficient of pass rate of the two methods was 0.782, which indicated that there was a positive correlation between the two test results. Besides, the detection results showed that ATP bioluminescence method had higher sensitivity than plate counting method. Therefore, ATP bioluminescence method was more suitable for the rapid detection of the colony of hospital health environment, and helps the hospital to better manage its environmental hygiene conditions. 

Use of a Rapid Microbial ATP Bioluminescence Assay to Detect Contamination on Beef and Pork Carcasses†

1995 ◽  
Vol 58 (7) ◽  
pp. 770-775 ◽  
Author(s):  
GREGORY R. SIRAGUSA ◽  
CATHERINE N. CUTTER ◽  
WARREN J. DORSA ◽  
MOHAMMAD KOOHMARAIE
Keyword(s):  
Plate Count ◽  
Assay Sensitivity ◽  
Standard Plate Count ◽  
Critical Control Points ◽  
Processing Plants ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Commercial Processing ◽  
Atp Bioluminescence Assay

A new microbial ATP bioluminescence assay was shown to be an accurate and rapid method to determine the levels of generic bacterial contamination on beef (n = 400 and pork (n = 320) carcasses sampled in commercial processing plants. Based on in vitro fecal dilution studies, the rapid microbial ATP (R-mATP) assay is as accurate as the standard plate count method for estimating bacteria in bovine or porcine fecal samples. The correlations (r) between the R-mATP assay and the standard aerobic plate count for beef and pork carcasses sampled in commercial processing were 0.91 and 0.93, respectively. A segmented-model statistical approach to determine the lower limits of assay sensitivity was developed. By using this model to analyze the in-plant data, the R-mATP test responded in a linear fashion to levels of microbial contamination of &gt; log10 2.0 aerobic CFU/cm2 on beef carcasses and of &gt; log10 3.2 aerobic CFU/cm2 for pork carcasses. The R-mATP assay requires approximately 5 min to complete, including sampling. Given the rapidity and accuracy of the assay, processors interested in monitoring critical control points in the slaughter process could potentially use the R-mATP assay to monitor microbiological prevention and intervention procedures for minimizing carcass contamination.

Comparison of a Rapid ATP Bioluminescence Assay and Standard Plate Count Methods for Assessing Microbial Contamination of Consumers' Refrigerators

2006 ◽  
Vol 69 (10) ◽  
pp. 2534-2538 ◽  
Author(s):  
FUR-CHI CHEN ◽  
SANDRIA L. GODWIN
Keyword(s):  
Microbial Contamination ◽  
Field Studies ◽  
Plate Count ◽  
Culture Methods ◽  
Psychrotrophic Bacteria ◽  
Standard Plate Count ◽  
Plate Count Agar ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay

The feasibility of using an ATP bioluminescence assay for assessing microbial contamination of home refrigerators was evaluated and compared with the standard culture methods. Samples of refrigerator surfaces were collected from 123 households by swabbing an area of 100 cm2 on three locations in the refrigerator with premoisturized sterile swabs. Microbial contaminations were determined by aerobic plate count (APC; incubated at 35°C for 48 h) and psychrotrophic plate count (PPC; incubated at 7°C for 10 days) on plate count agar. The results were compared to the readings from the microbial ATP (mATP) bioluminescence assay. The correlation coefficient (r) between mATP and PPC (r = 0.851) was slightly higher than that between mATP and APC (r = 0.823). Our results indicated a potential discrepancy in the population of mesophilic and psychrotrophic bacteria in the refrigerator samples. Nevertheless, mATP appeared to be a reliable indication of the average of APC and PPC (r = 0.895). The mATP bioluminescence assay would provide a rapid and convenient test for researchers in field studies to assess microbial contamination in refrigerators.

scholarly journals Efficiency of cleaning and disinfection of surfaces: correlation between assessment methods

2017 ◽  
Vol 70 (6) ◽  
pp. 1176-1183 ◽  
Author(s):  
Oleci Pereira Frota ◽  
Adriano Menis Ferreira ◽  
Odanir Garcia Guerra ◽  
Marcelo Alessandro Rigotti ◽  
Denise de Andrade ◽  
...  
Keyword(s):  
Comparative Study ◽  
Emergency Care ◽  
Visual Inspection ◽  
Significant Relationships ◽  
Colony Forming Units ◽  
Before And After ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay ◽  
Cleaning And Disinfection

ABSTRACT Objective: to assess the correlation among the ATP-bioluminescence assay, visual inspection and microbiological culture in monitoring the efficiency of cleaning and disinfection (C&D) of high-touch clinical surfaces (HTCS) in a walk-in emergency care unit. Method: a prospective and comparative study was carried out from March to June 2015, in which five HTCS were sampled before and after C&D by means of the three methods. The HTCS were considered dirty when dust, waste, humidity and stains were detected in visual inspection; when ≥2.5 colony forming units per cm2 were found in culture; when ≥5 relative light units per cm2 were found at the ATP-bioluminescence assay. Results: 720 analyses were performed, 240 per method. The overall rates of clean surfaces per visual inspection, culture and ATP-bioluminescence assay were 8.3%, 20.8% and 44.2% before C&D, and 92.5%, 50% and 84.2% after C&D, respectively (p<0.001). There were only occasional statistically significant relationships between methods. Conclusion: the methods did not present a good correlation, neither quantitative nor qualitatively.

scholarly journals Antibacterial Activity and Mechanism of Action of the Silver Ion in Staphylococcus aureus and Escherichia coli

2008 ◽  
Vol 74 (7) ◽  
pp. 2171-2178 ◽  
Author(s):  
Woo Kyung Jung ◽  
Hye Cheong Koo ◽  
Ki Woo Kim ◽  
Sook Shin ◽  
So Hyun Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Mechanism Of Action ◽  
Antibacterial Effect ◽  
Silver Ion ◽  
Plate Count ◽  
E Coli ◽  
Count Method ◽  
Plate Count Method ◽  
Conventional Plate

ABSTRACT The antibacterial effect and mechanism of action of a silver ion solution that was electrically generated were investigated for Staphylococcus aureus and Escherichia coli by analyzing the growth, morphology, and ultrastructure of the bacterial cells following treatment with the silver ion solution. Bacteria were exposed to the silver ion solution for various lengths of time, and the antibacterial effect of the solution was tested using the conventional plate count method and flow cytometric (FC) analysis. Reductions of more than 5 log10 CFU/ml of both S. aureus and E. coli bacteria were confirmed after 90 min of treatment with the silver ion solution. Significant reduction of S. aureus and E. coli cells was also observed by FC analysis; however, the reduction rate determined by FC analysis was less than that determined by the conventional plate count method. These differences may be attributed to the presence of bacteria in an active but nonculturable (ABNC) state after treatment with the silver ion solution. Transmission electron microscopy showed considerable changes in the bacterial cell membranes upon silver ion treatment, which might be the cause or consequence of cell death. In conclusion, the results of the present study suggest that silver ions may cause S. aureus and E. coli bacteria to reach an ABNC state and eventually die.

Adenosine Triphosphate Bioluminescence for Hygiene Monitoring in Health Care Institutions

1994 ◽  
Vol 57 (6) ◽  
pp. 509-513 ◽  
Author(s):  
KLAUS SEEGER ◽  
MANSEL W. GRIFFITHS
Keyword(s):  
Health Care ◽  
Adenosine Triphosphate ◽  
Meat Product ◽  
Plate Count ◽  
Standard Plate Count ◽  
Atp Assay ◽  
Health Care Institutions ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay

An investigation was conducted to assess the practical use of an adenosine triphosphate (ATP) bioluminescence assay to evaluate the effectiveness of cleaning and sanitizing meat slicers in eight health care institutions. The ATP bioluminescence assay was compared to conventional swabbing techniques using standard plate count to enumerate microbial load. Assays were performed on meat slicers before use, after slicing a meat product and after sanitizing. There was a general overall agreement in results obtained by both methods but the ATP assay gave a better indication of the cleanliness of the meat slicer as it was able to detect the presence of meat residues left on the blade after improper sanitation. Results were available within 5 min using the ATP bioluminescence method, thus providing an opportunity for immediate remedial action.

Comparison between the EC plate count test at 21 °C and an accelerated plate count method for assessing the keeping quality of pasteurized milk

1992 ◽  
Vol 59 (3) ◽  
pp. 431-436 ◽  
Author(s):  
Sarah A. Langford ◽  
Rohan G. Kroll
Keyword(s):  
Plate Count ◽  
Gram Negative ◽  
Rapid Methods ◽  
Pasteurized Milk ◽  
Psychrotrophic Bacteria ◽  
Commercial Interest ◽  
Count Method ◽  
Keeping Quality ◽  
Plate Count Method

The keeping quality of properly refrigerated pasteurized milk and cream is primarily determined by post-pasteurization contamination by Gram-negative psychrotrophic bacteria (Phillips et al. 1981; Schröder et al. 1982). Reliable and rapid methods of assessing the levels of contamination by these organisms are therefore of commercial interest.

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