Electrostatic theory of the assembly of PAMAM dendrimers and DNA

Biopolymers ◽  
2016 ◽  
Vol 105 (5) ◽  
pp. 276-286 ◽  
Author(s):  
Angelo Perico
Keyword(s):  
Pamam Dendrimers ◽  
Electrostatic Theory

The Effect of PAMAM Dendrimers on Mesenchymal Stem Cell Viability and Differentiation

2012 ◽  
Vol 19 (29) ◽  
pp. 4969-4975 ◽  
Author(s):  
M. Goncalves ◽  
R. Castro ◽  
J. Rodrigues ◽  
H. Tomas
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Viability ◽  
Pamam Dendrimers

Synthesis and Characterization of Ferrocenyl Carboxilic Surface-Functionalized Resorcinaren-PAMAM Dendrimers

2015 ◽  
Vol 19 (19) ◽  
pp. 1954-1960 ◽  
Author(s):  
Sandra Cortez-Maya ◽  
Elena Klimova ◽  
R. I. Puente Lee ◽  
Andres Borja-Miranda ◽  
Marcos Martinez-Garcia
Keyword(s):  
Pamam Dendrimers ◽  
Synthesis And Characterization

Targeting Breast Cancer Cells with G4 PAMAM Dendrimers and Valproic Acid Derivative Complexes

2020 ◽  
Vol 20 (15) ◽  
pp. 1857-1872
Author(s):  
Alberto M. Muñoz ◽  
Manuel J. Fragoso-Vázquez ◽  
Berenice P. Martel ◽  
Alma Chávez-Blanco ◽  
Alfonso Dueñas-González ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Cell Lines ◽  
Water Solubility ◽  
Md Simulations ◽  
Pamam Dendrimer ◽  
Pamam Dendrimers ◽  
Force Microscopy ◽  
Micromolar Concentration ◽  
Atomic Force

Background: Our research group has developed some Valproic Acid (VPA) derivatives employed as anti-proliferative compounds targeting the HDAC8 enzyme. However, some of these compounds are poorly soluble in water. Objective: Employed the four generations of Polyamidoamine (G4 PAMAM) dendrimers as drug carriers of these compounds to increase their water solubility for further in vitro evaluation. Methods: VPA derivatives were subjected to Docking and Molecular Dynamics (MD) simulations to evaluate their affinity on G4 PAMAM. Then, HPLC-UV/VIS, 1H NMR, MALDI-TOF and atomic force microscopy were employed to establish the formation of the drug-G4 PAMAM complexes. Results: The docking results showed that the amide groups of VPA derivatives make polar interactions with G4 PAMAM, whereas MD simulations corroborated the stability of the complexes. HPLC UV/VIS experiments showed an increase in the drug water solubility which was found to be directly proportional to the amount of G4 PAMAM. 1H NMR showed a disappearance of the proton amine group signals, correlating with docking results. MALDI-TOF and atomic force microscopy suggested the drug-G4 PAMAM dendrimer complexes formation. Discussion: In vitro studies showed that G4 PAMAM has toxicity in the micromolar concentration in MDAMB- 231, MCF7, and 3T3-L1 cell lines. VPA CF-G4 PAMAM dendrimer complex showed anti-proliferative properties in the micromolar concentration in MCF-7 and 3T3-L1, and in the milimolar concentration in MDAMB- 231, whereas VPA MF-G4 PAMAM dendrimer complex didn’t show effects on the three cell lines employed. Conclusion: These results demonstrate that G4 PAMAM dendrimers are capableof transporting poorly watersoluble aryl-VPA derivate compounds to increase its cytotoxic activity against neoplastic cell lines.

Chronic administration of nano-sized PAMAM dendrimers in vivo inhibits EGFR-ERK1/2-ROCK signaling pathway and attenuates diabetes-induced vascular remodeling and dysfunction

2019 ◽  
Vol 18 ◽  
pp. 78-89 ◽  
Author(s):  
Saghir Akhtar ◽  
Bindu Chandrasekhar ◽  
Mariam HM Yousif ◽  
Waleed Renno ◽  
Ibrahim F. Benter ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Vascular Remodeling ◽  
Chronic Administration ◽  
Pamam Dendrimers

scholarly journals Use of Half-Generation PAMAM Dendrimers (G0.5–G3.5) with Carboxylate End-Groups to Improve the DACHPtCl2 and 5-FU Efficacy as Anticancer Drugs

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2924
Author(s):  
Cláudia Camacho ◽  
Helena Tomás ◽  
João Rodrigues
Keyword(s):  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Anticancer Drug ◽  
Water Solubility ◽  
Cancer Cell Lines ◽  
Pamam Dendrimers ◽  
Binding Assays ◽  
End Groups ◽  
Ic50 Values

The DACHPtCl2 compound (trans-(R,R)-1,2-diaminocyclohexanedichloroplatinum(II)) is a potent anticancer drug with a broad spectrum of activity and is less toxic than oxaliplatin (trans-l-diaminocyclohexane oxalate platinum II), with which it shares the active metal fragment DACHPt. Nevertheless, due to poor water solubility, its use as a chemotherapeutic drug is limited. Here, DACHPtCl2 was conjugated, in a bidentate form, with half-generation PAMAM dendrimers (G0.5–G3.5) with carboxylate end-groups, and the resulting conjugates were evaluated against various types of cancer cell lines. In this way, we aimed at increasing the solubility and availability at the target site of DACHPt while potentially reducing the adverse side effects. DNA binding assays showed a hyperchromic effect compatible with DNA helix’s disruption upon the interaction of the metallodendrimers and/or the released active metallic fragments with DNA. Furthermore, the prepared DACHPt metallodendrimers presented cytotoxicity in a wide set of cancer cell lines used (the relative potency regarding oxaliplatin was in general high) and were not hemotoxic. Importantly, their selectivity for A2780 and CACO-2 cancer cells with respect to non-cancer cells was particularly high. Subsequently, the anticancer drug 5-FU was loaded in a selected metallodendrimer (the G2.5COO(DACHPt)16) to investigate a possible synergistic effect between the two drugs carried by the same dendrimer scaffold and tested for cytotoxicity in A2780cisR and CACO-2 cancer cell lines. This combination resulted in IC50 values much lower than the IC50 for 5-FU but higher than those found for the metallodendrimers without 5-FU. It seems, thus, that the metallic fragment-induced cytotoxicity dominates over the cytotoxicity of 5-FU in the set of considered cell lines.

scholarly journals Substantial cell apoptosis provoked by Naked PAMAM Dendrimers in HER2-positive human breast cancer via JNK and ERK1/ERK2 signalling pathways

2021 ◽  
Author(s):  
Hadeel Kheraldine ◽  
Ishita Gupta ◽  
Hashim Alhussain ◽  
Aayesha Jabeen ◽  
Farhan S. Cyprian ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Apoptosis ◽  
Human Breast Cancer ◽  
Pamam Dendrimers ◽  
Signalling Pathways ◽  
Human Breast ◽  
Her2 Positive

scholarly journals PAMAM Dendrimers Functionalised with an Anti-TNF α Antibody and Chondroitin Sulphate for Treatment of Rheumatoid Arthritis

2021 ◽  
pp. 111845
Author(s):  
Isabel Matos Oliveira ◽  
Cristiana Gonçalves ◽  
Eduarda Pinheiro Oliveira ◽  
Rosana Simón-Vázquez ◽  
Alain da Silva Morais ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Chondroitin Sulphate ◽  
Pamam Dendrimers ◽  
Tnf Α
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