High level of expression of recombinant human granulocyte-macrophage colony stimulating factor in transgenic rice cell suspension culture

2003 ◽  
Vol 82 (7) ◽  
pp. 778-783 ◽  
Author(s):  
Yun-Ji Shin ◽  
Shin-Young Hong ◽  
Tae-Ho Kwon ◽  
Yong-Suk Jang ◽  
Moon-Sik Yang
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Transgenic Rice ◽  
Cell Suspension Culture ◽  
Colony Stimulating Factor ◽  
Rice Cell Suspension Culture ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
High Level ◽  
Stimulating Factor

Expression of human growth hormone in transgenic rice cell suspension culture

2008 ◽  
Vol 27 (5) ◽  
pp. 885-891 ◽  
Author(s):  
Tae-Geum Kim ◽  
Moon-Yeoun Baek ◽  
Eun-Kyung Lee ◽  
Tae-Ho Kwon ◽  
Moon-Sik Yang
Keyword(s):  
Growth Hormone ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Transgenic Rice ◽  
Human Growth Hormone ◽  
Cell Suspension Culture ◽  
Human Growth ◽  
Rice Cell Suspension Culture

scholarly journals Effects of recombinant GM-CSF on the blast cells of acute myeloblastic leukemia

Blood ◽  
1986 ◽  
Vol 68 (1) ◽  
pp. 313-316 ◽  
Author(s):  
T Hoang ◽  
N Nara ◽  
G Wong ◽  
S Clark ◽  
MD Minden ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Adherent Cells ◽  
Colony Stimulating Factor ◽  
Response Curves ◽  
Self Renewal ◽  
Gm Csf ◽  
Blast Cells ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor

The effects of recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) were compared to those of media conditioned by the continuous bladder carcinoma line, HTB9 (HTB9-CM), using three criteria. First, both GM-CSF and HTB9-CM stimulated blast colony formation in methylcellulose cultures, patient-to-patient variations were seen in the dose-response curves, and GM-CSF was effective, but less so that HTB9-CM. Second, GM-CSF also enhanced growth of blast progenitors in suspension culture, indicating its capacity to support self-renewal. GM-CSF was as effective as HTB9-CM in the production of adherent cells during the growth of blast cells in suspension, a finding that is interpreted to mean that GM-CSF also supports postdeterministic events in blast differentiation. Finally, colonies growing in the presence of GM-CSF were not phenotypically different than those stimulated by HTB9-CM.

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