Metabolic model for acetate uptake by a mixed culture of phosphate- and glycogen-accumulating organisms under anaerobic conditions

1,2-dibromoethane (DBE) is a common environmental contaminant; it is potentially carcinogenic and has been detected in soil and groundwater supplies. Most of the biodegradation studies to date have been performed under anaerobic conditions or in the context of soil remediation, where the pollutant concentration was in the ppb range. The aim of this work was to find a bacterial culture (axenic or mixed) capable of complete aerobic mineralisation of concentrations of DBE up to 1 g L−1 under well controlled laboratory conditions. A mixed culture capable of degrading DBE as a sole source of carbon has been enriched. In order to verify biodegradation, formation of biodegradation products as well as the disappearance of DBE were measured. The ability of this culture to degrade other chlorinated and brominated compounds has also been tested. Successful degradation of bromoethane, bromoethanol and bromochloroethane was achieved.

Download Full-text

Pseudomonas aeruginosa PAO1 Pyocin Production Affects Population Dynamics within Mixed-Culture Biofilms

Journal of Bacteriology ◽

10.1128/jb.01458-08 ◽

2008 ◽

Vol 191 (4) ◽

pp. 1349-1354 ◽

Cited By ~ 39

Author(s):

Richard D. Waite ◽

Michael A. Curtis

Keyword(s):

Pseudomonas Aeruginosa ◽

Population Dynamics ◽

Mixed Culture ◽

Bacterial Population ◽

Anaerobic Conditions ◽

Strain Pao1 ◽

Pseudomonas Aeruginosa Pao1 ◽

Sensitive Isolate ◽

Slow Growing ◽

Aerobic And Anaerobic

ABSTRACT Transcriptomic and phenotypic studies showed that pyocins are produced in Pseudomonas aeruginosa PAO1 aerobic and anaerobic biofilms. Pyocin activity was found to be high in slow-growing anaerobic biofilms but transient in aerobic biofilms. Biofilm coculture of strain PAO1 and a pyocin-sensitive isolate showed that pyocin production had a significant impact on bacterial population dynamics, particularly under anaerobic conditions.

Download Full-text

Model-based analysis of anaerobic acetate uptake by a mixed culture of polyphosphate-accumulating and glycogen-accumulating organisms

Biotechnology and Bioengineering ◽

10.1002/bit.10671 ◽

2003 ◽

Vol 83 (3) ◽

pp. 293-302 ◽

Cited By ~ 36

Author(s):

Raymond J. Zeng ◽

Zhiguo Yuan ◽

J�rg Keller

Keyword(s):

Mixed Culture ◽

Model Based ◽

Acetate Uptake ◽

Model Based Analysis

Download Full-text

Initial Creavage of Propylene Glycol with Mixed Culture under Anaerobic Conditions

Journal of Korean Society of Water Science and Technology ◽

10.17640/kswst.2021.29.1.3 ◽

2021 ◽

Vol 29 (1) ◽

pp. 3-8

Author(s):

Jong Hyuk Seok

Keyword(s):

Propylene Glycol ◽

Mixed Culture ◽

Anaerobic Conditions

Download Full-text

Acetate uptake efficiency of polyphosphate-accumulating organisms under exposure to surfactants

Water Science & Technology ◽

10.2166/wst.2009.104 ◽

2009 ◽

Vol 60 (11) ◽

pp. 2775-2780

Author(s):

K. Tsuji ◽

M. Fujita ◽

H. Furumai

Keyword(s):

Municipal Wastewater ◽

Batch Reactor ◽

Alcohol Ethoxylates ◽

Anaerobic Conditions ◽

Linear Alkylbenzene ◽

Acetate Uptake ◽

Batch Tests ◽

Linear Alkylbenzene Sulfonates ◽

Negative Impacts ◽

Polyphosphate Accumulating Organisms

We investigated the influence of surfactants such as linear alkylbenzene sulfonates (LAS) and alcohol ethoxylates (AE) on acetate uptake by polyphosphate-accumulating organisms (PAOs) under anaerobic conditions, using the phosphate requirement for acetate uptake (+ΔP/−ΔAc ratio). In order to estimate the +ΔP/−ΔAc ratio, anaerobic batch tests were conducted using activated sludge collected from an anaerobic/oxic sequencing batch reactor used to treat municipal wastewater continuously supplemented with a detergent containing LAS and AE. We demonstrated that LAS and AE have both positive and negative impacts on acetate uptake by PAOs. The disadvantage is that long-term exposure to the detergent inhibits acetate uptake by PAOs, thus deteriorating the efficiency, even if the surfactants are no longer present during the tests. Furthermore, the existence of LAS and/or AE with acetate further diminishes the efficiency. The advantage is that LAS and AE are potential sources of polyhydroxyalkanoate for PAOs, because acetate is produced from the surfactants under anaerobic conditions.

Download Full-text