Establishment ofFUT8 knockout Chinese hamster ovary cells: An ideal host cell line for producing completely defucosylated antibodies with enhanced antibody-dependent cellular cytotoxicity

2004 ◽  
Vol 87 (5) ◽  
pp. 614-622 ◽  
Author(s):  
Naoko Yamane-Ohnuki ◽  
Satoko Kinoshita ◽  
Miho Inoue-Urakubo ◽  
Machi Kusunoki ◽  
Shigeru Iida ◽  
...  
Keyword(s):  
Host Cell ◽  
Cell Line ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Cellular Cytotoxicity ◽  
Antibody Dependent Cellular Cytotoxicity ◽  
Host Cell Line ◽  
Ovary Cells

Generation of Highly Productive Chinese Hamster Ovary Cells by Application of Metabolic Pool Selection During Cell Line Development

2019 ◽  
Vol 7 (5) ◽  
pp. 355-367
Author(s):  
Michael Thiele ◽  
Norbert Arnold ◽  
Axel J. Scheidig ◽  
Karsten Winkler
Keyword(s):  
Cell Line ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Cell Line Development ◽  
Ovary Cells ◽  
Metabolic Pool

scholarly journals New cell line development for antibody-producing Chinese hamster ovary cells using split green fluorescent protein

2012 ◽  
Vol 12 (1) ◽  
pp. 24 ◽  
Author(s):  
Yeon-Gu Kim ◽  
Byoungwoo Park ◽  
Jung Ahn ◽  
Joon-Ki Jung ◽  
Hong Lee ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Cell Line ◽  
Chinese Hamster Ovary ◽  
Fluorescent Protein ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Cell Line Development ◽  
Ovary Cells ◽  
New Cell Line ◽  
Green Fluorescent

scholarly journals Enhancement of cytotoxicities of ricin and Pseudomonas toxin in Chinese hamster ovary cells by nigericin.

1981 ◽  
Vol 1 (6) ◽  
pp. 552-559 ◽  
Author(s):  
B Ray ◽  
H C Wu
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Protein Synthesis ◽  
Cell Line ◽  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Diphtheria Toxin ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Pseudomonas Aeruginosa Exotoxin

Nigericin and monensin, ionophores for Na+ and K+, have been found to enhance the cytotoxicities of abrin, ricin, and Pseudomonas aeruginosa exotoxin A in Chinese hamster ovary (CHO) cells. They do not affect the cytotoxicity of diphtheria toxin in the same cell line. Maximal sensitization of the CHO cells toward ricin and Pseudomonas toxin requires preculture of CHO cells in the presence of nigericin. Inhibition of protein synthesis in CHO cells by ricin or Pseudomonas toxin is also enhanced by preculture of CHO cells in the presence of nigericin. These results suggest a common step in the intoxication process of ricin and Pseudomonas toxin, the rate of which is facilitated by pretreatment with nigericin. This step is, however, not shared by the intoxication of CHO cells with diphtheria toxin.

Pre-UV-Treatment of Cells Results in Enhanced Host Cell Reactivation of a UV Damaged Reporter Gene in CHO-AA8 Chinese Hamster Ovary Cells but Not in Transcription-Coupled Repair Deficient CHO-UV61 Cells

2004 ◽  
Vol 24 (6) ◽  
pp. 559-576 ◽  
Author(s):  
Lili Liu ◽  
Andrew J. Rainbow
Keyword(s):  
Host Cell ◽  
Reporter Gene ◽  
Chinese Hamster Ovary ◽  
Excision Repair ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Cell Reactivation ◽  
Host Cell Reactivation ◽  
Transcription Coupled Repair

We have used a non-replicating recombinant adenovirus, Ad5MCMVlacZ, which expresses the β-galactosidase reporter gene, to examine both constitutive and inducible repair of UV-damaged DNA in repair proficient CHO-AA8 Chinese hamster ovary cells and in mutant CHO-UV61 cells which are deficient in the transcription-coupled repair (TCR) pathway of nucleotide excision repair. Host cell reactivation (HCR) of β-galactosidase activity for UV-irradiated Ad5MCMVlacZ was significantly reduced in non-irradiated CHO-UV61 cells compared to that in non-irradiated CHO-AA8 cells suggesting that repair in the transcribed strand of the UV-damaged reporter gene in untreated cells utilizes TCR. Prior UV-irradiation of cells with low UV fluences resulted in a transient enhancement of HCR for expression of the UV-damaged reporter gene in CHO-AA8 cells but not in TCR deficient CHO-UV61 cells. These results suggest the presence of an inducible DNA pathway in CHO cells that results from an enhancement of TCR or a mechanism that involves the TCR pathway.

scholarly journals High-level stable expression of recombinant 5-HT1A 5-hydroxytryptamine receptors in Chinese hamster ovary cells

1992 ◽  
Vol 285 (3) ◽  
pp. 933-938 ◽  
Author(s):  
A Newman-Tancredi ◽  
R Wootton ◽  
P G Strange
Keyword(s):  
Adenylate Cyclase ◽  
Cell Line ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Receptor Gene ◽  
Chinese Hamster ◽  
Receptor Expression ◽  
Ovary Cells ◽  
Recombinant Cell ◽  
High Level

The human 5-hydroxytryptamine 5-HT1A receptor gene was transfected into Chinese hamster ovary cells. A series of recombinant monoclonal cell lines expressing the receptor were isolated and the properties of one cell line that expressed receptors at a high level (2.8 pmol/mg) were studied in detail. In ligand binding assays with the selective 5-HT1A receptor agonist 2-(NN-di[3H]propylamino)-8-hydroxy-1,2,3,4-tetrahydronaphthalene ([3H]8-OH-DPAT) only a single class of saturable high-affinity binding sites was detected, with a pharmacological profile in competition experiments essentially identical to that of the 5-HT1A receptor of bovine hippocampus. [3H]8-OH-DPAT binding to the recombinant cell membranes was inhibited by GTP, showing that the receptors in the transfected cells couple to G-proteins. A series of 5-hydroxytryptamine agonists inhibited forskolin-stimulated adenylate cyclase activity in the cells and, despite the high level of receptor expression, their apparent efficacies were similar to those observed for inhibition of adenylate cyclase in brain. This recombinant cell line provides a complete model system for studying the 5-HT1A receptor and its transmembrane signalling system. The recombinant cells can also be grown in suspension culture for long periods but, whereas 5-HT1A receptor numbers and receptor regulation by guanine nucleotides are maintained in suspension-grown cells, the inhibition of adenylate cyclase by the 5-HT1A receptor is gradually lost.

N-Glycosylation and in vitro enzymic activity of human recombinant tissue plasminogen activator expressed in Chinese hamster ovary cells and a murine cell line

Biochemistry ◽  
1989 ◽  
Vol 28 (19) ◽  
pp. 7670-7679 ◽  
Author(s):  
Raj B. Parekh ◽  
Raymond A. Dwek ◽  
Pauline M. Rudd ◽  
Jerry R. Thomas ◽  
Thomas W. Rademacher ◽  
...  
Keyword(s):  
Cell Line ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Recombinant Tissue Plasminogen Activator ◽  
Enzymic Activity ◽  
Ovary Cells ◽  
Murine Cell Line ◽  
Tissue Plasminogen

Generation of an industrially ideal host cell line for producing completely-defucosylated antibody with enhanced antibody-dependent cellular cytotoxicity (ADCC)

2006 ◽  
pp. 39-46
Author(s):  
Mitsuo Satoh ◽  
Naoko Yamane-Ohnuki ◽  
Katsuhiro Mori ◽  
Ripei Niwa ◽  
Toyohide Shinkawa ◽  
...  
Keyword(s):  
Host Cell ◽  
Cell Line ◽  
Cellular Cytotoxicity ◽  
Antibody Dependent Cellular Cytotoxicity ◽  
Host Cell Line

Enhancement of cytotoxicities of ricin and Pseudomonas toxin in Chinese hamster ovary cells by nigericin

1981 ◽  
Vol 1 (6) ◽  
pp. 552-559
Author(s):  
B Ray ◽  
H C Wu
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Protein Synthesis ◽  
Cell Line ◽  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Diphtheria Toxin ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Pseudomonas Aeruginosa Exotoxin

Nigericin and monensin, ionophores for Na+ and K+, have been found to enhance the cytotoxicities of abrin, ricin, and Pseudomonas aeruginosa exotoxin A in Chinese hamster ovary (CHO) cells. They do not affect the cytotoxicity of diphtheria toxin in the same cell line. Maximal sensitization of the CHO cells toward ricin and Pseudomonas toxin requires preculture of CHO cells in the presence of nigericin. Inhibition of protein synthesis in CHO cells by ricin or Pseudomonas toxin is also enhanced by preculture of CHO cells in the presence of nigericin. These results suggest a common step in the intoxication process of ricin and Pseudomonas toxin, the rate of which is facilitated by pretreatment with nigericin. This step is, however, not shared by the intoxication of CHO cells with diphtheria toxin.

Sign in / Sign up

Export Citation Format

Share Document