The influence of grafted polymer architecture and fluid hydrodynamics on protein separation by entropic interaction chromatography

2007 ◽  
Vol 97 (3) ◽  
pp. 574-587 ◽  
Author(s):  
Bryan R. Coad ◽  
Bradley M. Steels ◽  
Jayachandran N. Kizhakkedathu ◽  
Donald E. Brooks ◽  
Charles A. Haynes
Keyword(s):  
Protein Separation ◽  
Polymer Architecture ◽  
Grafted Polymer ◽  
Fluid Hydrodynamics

Phenylboronic acid-sugar grafted polymer architecture as a dual stimuli-responsive gene carrier for targeted anti-angiogenic tumor therapy

Biomaterials ◽  
2016 ◽  
Vol 75 ◽  
pp. 102-111 ◽  
Author(s):  
Jinhwan Kim ◽  
Yeong Mi Lee ◽  
Hyunwoo Kim ◽  
Dongsik Park ◽  
Jihoon Kim ◽  
...  
Keyword(s):  
Phenylboronic Acid ◽  
Tumor Therapy ◽  
Gene Carrier ◽  
Stimuli Responsive ◽  
Polymer Architecture ◽  
Grafted Polymer ◽  
Responsive Gene

scholarly journals Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: Types of ion exchange adsorbent and grafted polymer architecture

2018 ◽  
Vol 41 (10) ◽  
pp. 2119-2129
Author(s):  
Hon Wei Ng ◽  
Micky Fu Xiang Lee ◽  
Gek Kee Chua ◽  
Bee Koon Gan ◽  
Wen Siang Tan ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Ion Exchange ◽  
Hepatitis B ◽  
Polymer Architecture ◽  
Grafted Polymer ◽  
Virus Like Particle ◽  
B Virus ◽  
Flow Through

Exploration of Neem Gum, Acrylamide Grafted Neem Gum and Carboxymethylated Neem Gum as Retardant in Tablet Formulation

2020 ◽  
Vol 16 (9) ◽  
pp. 1404-1410
Author(s):  
Rishabha Malviya
Keyword(s):  
Diclofenac Sodium ◽  
Tablet Formulation ◽  
Angle Of Repose ◽  
Wet Granulation ◽  
Binding Agent ◽  
Grafted Polymer ◽  
Model Drug ◽  
Tablet Dosage ◽  
Tapped Density ◽  
Derivatives Of

Background: In the previous study, investigators have synthesized acrylamide grafted and carboxymethylated derivatives of neem gum and evaluated their potential in the formulation of nanoparticles. In continuation of previous work, authors have evaluated neem gum polysaccharide (NGP), acrylamide grafted neem gum polysaccharide (NGP-g-Am) and carboxymethylated neem gum polysaccharide (CMNGP) as binding agent in the tablet dosage form. Methods: Diclofenac sodium was used as a model drug while microcrystalline cellulose and talc were used as excipient in the preparation of granules employing wet granulation technique. NGP, NGP-g-Am and CMNGP were utilized as binding agent in the preparation of granules. Prepared granules were characterized for various pre-compression and post-compression parameters. Results and Discussion: Binding agents were used in the concentration of 4-24%w/w. NGP incorporated granules showed more bulk density and lower values of tapped density, Carr’s index, bulkiness, Hausner’s ratio and angle of repose as compared to NGP-g-Am consisting granules. NGP-g-Am consisting tablets showed more hardness and zero friability as compared to NGP based tablets. Drug content was found lower for the tablets having grafted polymer in place of NGP. CMNGP were also utilized to prepare granules but granules were not be able to compress keeping all the compacting parameters same as used in the case of NGP and NGP-g-Am consisting granules. NGP and NGP-g-Am were able to sustain drug release up to 6 and 8 h, respectively. Conclusion: It can be concluded that NGP-g-Am induces better properties when used as a binder in the tablet formulation than native polymer, while CMNGP cannot be utilized as a binding agent in the preparation of a tablet.

scholarly journals Computer-supported Detection of M-Components and Evaluation of Immunoglobulins after Capillary Electrophoresis

2001 ◽  
Vol 47 (1) ◽  
pp. 110-117 ◽  
Author(s):  
Magnus Jonsson ◽  
Joyce Carlson ◽  
Jan-Olof Jeppsson ◽  
Per Simonsson
Keyword(s):  
Capillary Electrophoresis ◽  
Decision Support ◽  
Protein Separation ◽  
Serum Proteins ◽  
Cost Effective ◽  
Clinical Samples ◽  
Serum Samples ◽  
Computerized Decision Support ◽  
Cost Effective Method ◽  
Mathematical Algorithms

Abstract Background: Electrophoresis of serum samples allows detection of monoclonal gammopathies indicative of multiple myeloma, Waldenström macroglobulinemia, monoclonal gammopathy of undetermined significance, and amyloidosis. Present methods of high-resolution agarose gel electrophoresis (HRAGE) and immunofixation electrophoresis (IFE) are manual and labor-intensive. Capillary zone electrophoresis (CZE) allows rapid automated protein separation and produces digital absorbance data, appropriate as input for a computerized decision support system. Methods: Using the Beckman Paragon CZE 2000 instrument, we analyzed 711 routine clinical samples, including 95 monoclonal components (MCs) and 9 cases of Bence Jones myeloma, in both the CZE and HRAGE systems. Mathematical algorithms developed for the detection of monoclonal immunoglobulins (MCs) in the γ- and β-regions of the electropherogram were tested on the entire material. Additional algorithms evaluating oligoclonality and polyclonal concentrations of immunoglobulins were also tested. Results: CZE electropherograms corresponded well with HRAGE. Only one IgG MC of 1 g/L, visible on HRAGE, was not visible after CZE. Algorithms detected 94 of 95 MCs (98.9%) and 100% of those visible after CZE. Of 607 samples lacking an MC on HRAGE, only 3 were identified by the algorithms (specificity, 99%). Algorithms evaluating total gammaglobulinemia and oligoclonality also identified several cases of Bence Jones myeloma. Conclusions: The use of capillary electrophoresis provides a modern, rapid, and cost-effective method of analyzing serum proteins. The additional option of computerized decision support, which provides rapid and standardized interpretations, should increase the clinical availability and usefulness of protein analyses in the future.

Nanoporous polyethersulfone membranes prepared by mixed solvent phase separation method for protein separation

2021 ◽  
pp. 119507
Author(s):  
Peipei Li ◽  
Roshni L. Thankamony ◽  
Xiang Li ◽  
Zhen Li ◽  
Xiaowei Liu ◽  
...  
Keyword(s):  
Phase Separation ◽  
Protein Separation ◽  
Mixed Solvent ◽  
Separation Method ◽  
Solvent Phase

Selective Preconcentration of U(VI) and Th(IV) in Trace and Macroscopic Levels Using Malonamide Grafted Polymer from Acidic Matrices

2005 ◽  
Vol 150 (3-4) ◽  
pp. 297-304 ◽  
Author(s):  
Chinthalapati Siva Kesava Raju ◽  
Mandakolathur S. Subramanian
Keyword(s):  
Grafted Polymer ◽  
Selective Preconcentration

Graft Polymers Derived from Natural Rubber

1956 ◽  
Vol 29 (1) ◽  
pp. 99-105 ◽  
Author(s):  
G. F. Bloomfield ◽  
F. M. Merrett ◽  
F. J. Popham ◽  
P. Mc L. Swift
Keyword(s):  
Tensile Strength ◽  
Natural Rubber ◽  
Methyl Methacrylate ◽  
Transfer Reaction ◽  
Polymer Chains ◽  
Vinyl Monomers ◽  
Grafted Polymer ◽  
Polymeric Chains ◽  
Graft Polymers ◽  
Direct Growth

Abstract Graft polymers result when vinyl monomers are polymerized in the presence of natural rubber, either in solution or as latex, and some of the polymeric chains become attached to the rubber molecules. The properties of the natural rubber can be widely modified according to the nature and the amount of the grafted polymer. The polymer-modified natural rubber appears to be produced by direct growth of polymer chains on to rubber molecules rather than by a transfer reaction involving the rubber. Graft polymers of styrene and methyl methacrylate with natural rubber can be compounded and cured to give light-colored articles of good tensile strength, and rubber-methyl methacrylate graft polymers have outstanding flex-cracking and fatigue resistance.

scholarly journals Membrane manufacture for peptide separation

2016 ◽  
Vol 18 (19) ◽  
pp. 5151-5159 ◽  
Author(s):  
DooLi Kim ◽  
Octavio R. Salazar ◽  
Suzana Pereina Nunes
Keyword(s):  
Ionic Liquids ◽  
Protein Separation ◽  
Biomedical Applications ◽  
Food Industry ◽  
Green Solvent ◽  
Seawater Desalination ◽  
Peptide Separation ◽  
Polysulfone Membranes

Polysulfone membranes are key tools in biomedical applications, such as hemodialysis and protein separation, in the food industry, and in seawater desalination. Ionic liquids are proposed as green solvent for membrane manufacture with tailored peptide selectivity.

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