Nanoporous polyethersulfone membranes prepared by mixed solvent phase separation method for protein separation

2021 ◽  
pp. 119507
Author(s):  
Peipei Li ◽  
Roshni L. Thankamony ◽  
Xiang Li ◽  
Zhen Li ◽  
Xiaowei Liu ◽  
...  
Keyword(s):  
Phase Separation ◽  
Protein Separation ◽  
Mixed Solvent ◽  
Separation Method ◽  
Solvent Phase

Fabrication of NF-PLLA Scaffolds by Gel Extraction Phase Separation

2014 ◽  
Vol 1033-1034 ◽  
pp. 1090-1093
Author(s):  
Zhi Hang He ◽  
Jia Liu ◽  
Guo Jie Xu ◽  
Jian Jin Jiao ◽  
Xiu Feng Xiao
Keyword(s):  
Phase Separation ◽  
Bone Tissue ◽  
Mixed Solvent ◽  
Solvent System ◽  
Separation Method ◽  
Interconnected Network ◽  
Nanofibrous Scaffolds ◽  
And Performance ◽  
Gel Temperature ◽  
Extraction Phase

The 1, 4-dioxane/ethanol mixed solvent was used as solvent system, and the gel extraction phase separation method was adopted to prepare porous PLLA nanofibrous scaffolds. At that, we studied the effect of using different gel temperature to influence nanofibrous PLLA scaffolds’ structure and performance. The results showed that the morphology of nanofibers and the diameter of fibers could be regulated by gel temperature; a lower gel temperature resulted in higher crystallinity of PLLA scaffold. In addition, the PLLA scaffolds have excellent interconnected network when the gel temperature was in the range of-20 to-10°C. The PLLA scaffolds will become a material to repair the bone tissue, hopefully.

Phase separation in sol gel-derived ceramic films of silica-zirconia, alumina-zirconia, and titania-zirconia system

1994 ◽  
Vol 52 ◽  
pp. 646-647
Author(s):  
J. Tong ◽  
L. Eyring
Keyword(s):  
Fracture Toughness ◽  
Phase Separation ◽  
Sol Gel ◽  
Great Influence ◽  
High Strength ◽  
Chemical Durability ◽  
Separation Method ◽  
Toughening Mechanism ◽  
Ceramic Films ◽  
Zirconia Toughened Alumina

There is increasing interest in composites containing zirconia because of their high strength, fracture toughness, and its great influence on the chemical durability in glass. For the zirconia-silica system, monolithic glasses, fibers and coatings have been obtained. There is currently a great interest in designing zirconia-toughened alumina including exploration of the processing methods and the toughening mechanism.The possibility of forming nanocrystal composites by a phase separation method has been investigated in three systems: zirconia-alumina, zirconia-silica and zirconia-titania using HREM. The morphological observations initially suggest that the formation of nanocrystal composites by a phase separation method is possible in the zirconia-alumina and zirconia-silica systems, but impossible in the zirconia-titania system. The separation-produced grain size in silica-zirconia system is around 5 nm and is more uniform than that in the alumina-zirconia system in which the sizes of the small polyhedron grains are around 10 nm. In the titania-zirconia system, there is no obvious separation as was observed in die alumina-zirconia and silica-zirconia system.

scholarly journals FORMULATION AND IN VITRO, IN VIVO EVALUATION OF PRONIOSOMAL GEL OF NEOMYCIN SULPHATE

2019 ◽  
pp. 156-163
Author(s):  
AMOL SHETE ◽  
PRIYANKA THORAT ◽  
RAJENDRA DOIJAD ◽  
SACHIN SAJANE
Keyword(s):  
Phase Separation ◽  
Skin Irritation ◽  
Entrapment Efficiency ◽  
Separation Method ◽  
Gelling Agent ◽  
In Vivo Evaluation ◽  
Skin Delivery ◽  
Span 60

Objective: The objectives of present investigation were to prepare and evaluate proniosomes of neomycin sulphate (NS) by coacervation phase separation method by using sorbitan monostearate (span 60) and lecithin as a surfactant to increase the penetration through the skin and study the effect of concentration of the same. Methods: Proniosomes of neomycin sulphate (NS) were prepared by coacervation phase separation method by using span 60 and lecithin. The effect of concentration of span 60 and lecithin was studied by factorial design. The prepared proniosomes were converted to gel by using carbopol as a gelling agent. The prepared formulations were evaluated for entrapment efficiency, in vitro drug diffusion, in vitro antibacterial activity and in vivo skin irritation test etc. Results: All Formulation showed the percentage entrapment efficiency in the range 38.31±0.05% to 77.96±0.06%, good homogeneity and gel was easily spreadable with minimal of shear. Optimized formulation showed enhanced rate of diffusion in vitro, increase in zone of inhibition against staphylococcus aureus, no skin irritation and showed good stability. Conclusion: The results of present study indicates that proniosomal gel formulated by using combination of span 60, Lecithin, cholesterol can be used to enhance skin delivery of NS because of excellent permeation of drug. Developed proniosomal gel formulation was promising carrier for NS

Porous electrospun ultrafine fibers via a liquid–liquid phase separation method

2012 ◽  
Vol 291 (5) ◽  
pp. 1293-1296 ◽  
Author(s):  
Zhimei Wei ◽  
Quanchao Zhang ◽  
Lihua Wang ◽  
Xiaojun Wang ◽  
Shengru Long ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Liquid Phase Separation ◽  
Separation Method ◽  
Ultrafine Fibers ◽  
Liquid Liquid Phase Separation

Structure–property relation of porous poly (l-lactic acid) scaffolds fabricated using organic solvent mixtures and controlled cooling rates and its bio-compatibility with human adipose stem cells

2018 ◽  
Vol 33 (4) ◽  
pp. 397-415 ◽  
Author(s):  
Harish Chinnasami ◽  
Jeff Gimble ◽  
Ram V Devireddy
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Lactic Acid ◽  
Phase Separation ◽  
Mechanical Strength ◽  
Separation Method ◽  
Thermally Induced Phase Separation ◽  
Cooling Rates ◽  
Thermally Induced ◽  
Pore Sizes

Thermally induced phase separation method was used to make porous three-dimensional poly (l-lactic acid) scaffolds. The effect of imposed thermal profile during freezing of the poly (l-lactic acid) in dioxane solution on the scaffold was characterized by their micro-structure, porosity (%), pore sizes’ distribution, and mechanical strength. The porosity (%) decreased considerably with increasing concentrations of poly (l-lactic acid) in the solution, while a decreasing trend was observed with increasing cooling rates. The mechanical strength increases with increase in poly (l-lactic acid) concentration and also with increase in the cooling rate for both types of solvents. Therefore, mechanical strength was increased by higher cooling rates while the porosity (%) remained relatively consistent. Scaffolds made using higher concentrations of poly (l-lactic acid; 7% and 10% w/v) in solvent showed better mechanical strength which improved relatively with increasing cooling rates (1°C–40°C/min). This phenomenon of enhanced structural integrity with increasing cooling rates was more prominent in scaffolds made from higher initial poly (l-lactic acid) concentrations. Human adipose–derived stem cells were cultured on these scaffold (7% and 10% w/v) prepared by thermally induced phase separation at all cooling rates to measure the cell proliferation efficiency as a function of their micro-structural properties. Mean pore sizes played a crucial role in cell proliferation than percent porosity since all scaffolds were >88% porous. The viability percent of human adipose tissue–derived adult stem cells increased consistently with longer periods of culture. Thus, poly (l-lactic acid) scaffolds prepared by thermally controlled thermally induced phase separation method could be a prime candidate for making ex vivo tissue-engineered grafts for surgical implantation.

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