scholarly journals A perfusable 3D cell-matrix tissue culture chamber for in situ evaluation of nanoparticle vehicle penetration and transport

2008 ◽  
Vol 99 (6) ◽  
pp. 1490-1501 ◽  
Author(s):  
Chee Ping Ng ◽  
Suzie Hwang Pun
Keyword(s):  
Tissue Culture ◽  
Culture Chamber ◽  
Cell Matrix

The use of Lab-Tek tissue culture Chamber/ Slides and Flaskette for processing cultured monolayers for electron microscopy

1983 ◽  
Vol 41 ◽  
pp. 626-627
Author(s):  
K. Chien ◽  
R.L. Van De Velde ◽  
R.C. Heusser ◽  
J.W. Said
Keyword(s):  
Tissue Culture ◽  
Glass Slide ◽  
Culture Chamber ◽  
Major Difficulty ◽  
Cell Monolayers ◽  
Plastic Resin ◽  
Fast Procedure ◽  
In Situ Embedding ◽  
Chamber Slide

In situ embedding is a preferable means for the ultrastructural study of cultured cell monolayers. However, the major difficulty is the separation of the polymerized plastic resin from the supporting substrates. This abstract demonstrates a simple, fast procedure for using existing products for cell culture and in situ embedding of monolayers. Epoxy block surfaces up to 10 cm2 can be easily separated from an untreated glass slide and sectioned vertically or horizontally for EM studies.The Lab-Tek tissue culture Chamber/Slides or Flaskette are constructed so that 1 to 8 chamber compartments or a single flask is attached to a regular glass slide by gaskets. Different cell lines or procedures can be performed on the same chamber/slide. When the plastic chambers and gasket are removed, the glass slide can be coverslipped and filed for reference. We use these products routinely for cell cultures. When an EM study is needed, we fix the cell monolayers in situ within the chambers or flask, dehydrate with ethanol, infiltrate and embed in an epoxy resin.

Smart Tissue Culture: in Situ Monitoring of the Activity of Protease Enzymes Secreted from Live Cells Using Nanostructured Photonic Crystals

Nano Letters ◽  
2009 ◽  
Vol 9 (5) ◽  
pp. 2021-2025 ◽  
Author(s):  
Kristopher A. Kilian ◽  
Leo M. H. Lai ◽  
Astrid Magenau ◽  
Siân Cartland ◽  
Till Böcking ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Photonic Crystals ◽  
In Situ Monitoring ◽  
Live Cells

scholarly journals NewIn SituCapture Quantitative (Real-Time) Reverse Transcription-PCR Method as an Alternative Approach for Determining Inactivation of Tulane Virus

2014 ◽  
Vol 80 (7) ◽  
pp. 2120-2124 ◽  
Author(s):  
Dapeng Wang ◽  
Shuxia Xu ◽  
David Yang ◽  
Glenn M. Young ◽  
Peng Tian
Keyword(s):  
Tissue Culture ◽  
Reverse Transcription ◽  
Viral Genome ◽  
Viral Infectivity ◽  
Qrt Pcr ◽  
Reverse Transcription Pcr ◽  
Alternative Approach ◽  
Tulane Virus ◽  
Pcr Method

ABSTRACTHuman noroviruses (HuNoVs) are the major cause of epidemic nonbacterial gastroenteritis. Although quantitative (real-time) reverse transcription-PCR (qRT-PCR) is widely used for detecting HuNoVs, it only detects the presence of viral RNA and does not indicate viral infectivity. Human blood group antigens (HBGAs) have been identified as receptors/co-receptors for both HuNoVs and Tulane virus (TV) and are crucial for viral infection. We propose that viral infectivity can be evaluated with a molecular assay based on receptor-captured viruses. In this study, we employed TV as an HuNoV surrogate to validate the HBGA-based capture qRT-PCR method against the 50% tissue culture infectious dose (TCID50) method. We employed type B HBGA on an immuno-well module to concentrate TV, followed by amplification of the captured viral genome byin situqRT-PCR. We first demonstrated that thisin situcapture qRT-PCR (ISC-qRT-PCR) method could effectively concentrate and detect TV. We then treated TV under either partial or full inactivation conditions and measured the remaining infectivity by ISC-qRT-PCR and a tissue culture-based amplification method (TCID50). We found that the ISC-qRT-PCR method could be used to evaluate virus inactivation deriving from damage to the capsid and study interactions between the capsid and viral receptor. Heat, chlorine, and ethanol treatment primarily affect the capsid structure, which in turns affects the ability of the capsid to bind to viral receptors. Inactivation of the virus by these methods could be reflected by the ISC-qRT-PCR method and confirmed by TCID50assay. However, the loss of the infectivity caused by damage to the viral genome (such as that from UV irradiation) could not be effectively reflected by this method. Despite this limitation, the ISC-qRT-PCR provides an alternative approach to determine inactivation of Tulane virus. A particular advantage of the ISC-qRT-PCR method is that it is also a faster and easier method to effectively recover and detect the viruses, as there is no need to extract viral RNA or to transfer the captured virus from magnetic beads to PCR tubes for further amplification. Therefore, ISC-qRT-PCR can be easily adapted for use in automated systems for multiple samples.

scholarly journals Differential expression of multiple cell-surface heparan sulfate proteoglycans during embryonic tooth development.

1994 ◽  
Vol 42 (8) ◽  
pp. 1043-1054 ◽  
Author(s):  
X M Bai ◽  
B Van der Schueren ◽  
J J Cassiman ◽  
H Van den Berghe ◽  
G David
Keyword(s):  
Cell Surface ◽  
Heparan Sulfate ◽  
Differential Expression ◽  
Tooth Development ◽  
Multiple Forms ◽  
Dental Tissues ◽  
Cell Matrix ◽  
Heparin Sulfate ◽  
Multiple Cell

Heparan sulfate accumulates on cell surfaces and at cell-matrix interfaces, and functionally modulates several of the effector molecules that support the interactions, growth, and differentiation of developing tissues. Using heparin sulfate-specific monoclonal antibodies MAb, we obtained evidence that extracts from rodent embryos contain multiple forms of cell surface-associated heparan sulfate proteoglycan (PG). Taking tooth development in the mouse embryo as a model to further investigate the relevance of this PG redundancy and using MAb against heparan sulfate, antibodies specific for syndecan (syndecan-1) and fibroglycan (syndecan-2) (two distinct members of a larger family of cell-surface heparan sulfate PGs), and specific cDNA probes for these two cell-surface PGs, we obtained in situ evidence for regulated and differential expression of multiple cell-surface heparan sulfate PGs. The unique, distinctive, and coordinated changes in the expressions of these PGs during morphogenesis and differentiation of dental tissues suggest that the various cell-surface PGs are not truly redundant but play important, specific, and potentially complementary roles during embryonic development.

The synovial cavity as a “tissue culture in situ”—Science or nonsense?

1982 ◽  
Vol 7 (2) ◽  
pp. 196-199 ◽  
Author(s):  
Austin D. Potenza ◽  
Mary C. Herte
Keyword(s):  
Tissue Culture
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