Systematic engineering of phytochelatin synthesis and arsenic transport for enhanced arsenic accumulation inE. coli

2009 ◽  
pp. n/a-n/a ◽  
Author(s):  
Shailendra Singh ◽  
Seung Hyun Kang ◽  
Wonkyu Lee ◽  
Ashok Mulchandani ◽  
Wilfred Chen
Keyword(s):  
Arsenic Accumulation ◽  
Arsenic Transport

Exogenous IAA Alleviates Arsenic Toxicity to Rice and Reduces Arsenic Accumulation in Rice Grains

2021 ◽  
Author(s):  
Yue He ◽  
Ting Zhang ◽  
Yu Sun ◽  
Xuejing Wang ◽  
Qingqing Cao ◽  
...  
Keyword(s):  
Arsenic Toxicity ◽  
Rice Grains ◽  
Arsenic Accumulation ◽  
Exogenous Iaa

scholarly journals Chemical intervention for enhancing growth and reducing grain arsenic accumulation in rice

2021 ◽  
Vol 276 ◽  
pp. 116719
Author(s):  
Ashish Kumar Srivastava ◽  
Manish Pandey ◽  
Tejashree Ghate ◽  
Vikash Kumar ◽  
Munish Kumar Upadhyay ◽  
...  
Keyword(s):  
Arsenic Accumulation

scholarly journals A molecular switch in sulfur metabolism to reduce arsenic and enrich selenium in rice grain

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sheng-Kai Sun ◽  
Xuejie Xu ◽  
Zhong Tang ◽  
Zhu Tang ◽  
Xin-Yuan Huang ◽  
...  
Keyword(s):  
Grain Quality ◽  
Sulfur Metabolism ◽  
Physical Interaction ◽  
Rice Grain ◽  
Yield Losses ◽  
Serine Acetyltransferase ◽  
Cysteine Synthase ◽  
Arsenic Tolerance ◽  
Arsenic Accumulation ◽  
Low Levels

AbstractRice grains typically contain high levels of toxic arsenic but low levels of the essential micronutrient selenium. Anthropogenic arsenic contamination of paddy soils exacerbates arsenic toxicity in rice crops resulting in substantial yield losses. Here, we report the identification of the gain-of-function arsenite tolerant 1 (astol1) mutant of rice that benefits from enhanced sulfur and selenium assimilation, arsenic tolerance, and decreased arsenic accumulation in grains. The astol1 mutation promotes the physical interaction of the chloroplast-localized O-acetylserine (thiol) lyase protein with its interaction partner serine-acetyltransferase in the cysteine synthase complex. Activation of the serine-acetyltransferase in this complex promotes the uptake of sulfate and selenium and enhances the production of cysteine, glutathione, and phytochelatins, resulting in increased tolerance and decreased translocation of arsenic to grains. Our findings uncover the pivotal sensing-function of the cysteine synthase complex in plastids for optimizing stress resilience and grain quality by regulating a fundamental macronutrient assimilation pathway.

Cadmium and arsenic accumulation during the rice growth period under in situ remediation

2019 ◽  
Vol 171 ◽  
pp. 451-459 ◽  
Author(s):  
Jiao-feng Gu ◽  
Hang Zhou ◽  
Hui-ling Tang ◽  
Wen-tao Yang ◽  
Min Zeng ◽  
...  
Keyword(s):  
Growth Period ◽  
In Situ Remediation ◽  
Rice Growth ◽  
Arsenic Accumulation

Decreases in arsenic accumulation by the plasma membrane intrinsic protein PIP2;2 in Arabidopsis and yeast

2021 ◽  
Vol 275 ◽  
pp. 116646
Author(s):  
Mahsa Modareszadeh ◽  
Ramin Bahmani ◽  
DongGwan Kim ◽  
Seongbin Hwang
Keyword(s):  
Plasma Membrane ◽  
Plasma Membrane Intrinsic Protein ◽  
Intrinsic Protein ◽  
Arsenic Accumulation

scholarly journals Enhanced Arsenic Accumulation in Engineered Bacterial Cells Expressing ArsR

2004 ◽  
Vol 70 (8) ◽  
pp. 4582-4587 ◽  
Author(s):  
Jan Kostal ◽  
Rosanna Yang ◽  
Cindy H. Wu ◽  
Ashok Mulchandani ◽  
Wilfred Chen
Keyword(s):  
Cell Growth ◽  
Environmental Protection Agency ◽  
Arsenic Removal ◽  
Fusion Partner ◽  
Resting Cells ◽  
Bacterial Cells ◽  
High Affinity ◽  
Affinity Ligand ◽  
Arsenic Accumulation ◽  
Severe Reduction

ABSTRACT The metalloregulatory protein ArsR, which offers high affinity and selectivity toward arsenite, was overexpressed in Escherichia coli in an attempt to increase the bioaccumulation of arsenic. Overproduction of ArsR resulted in elevated levels of arsenite bioaccumulation but also a severe reduction in cell growth. Incorporation of an elastin-like polypeptide as the fusion partner to ArsR (ELP153AR) improved cell growth by twofold without compromising the ability to accumulate arsenite. Resting cells overexpressing ELP153AR accumulated 5- and 60-fold-higher levels of arsenate and arsenite than control cells without ArsR overexpression. Conversely, no significant improvement in Cd2+ or Zn2+ accumulation was observed, validating the specificity of ArsR. The high affinity of ArsR allowed 100% removal of 50 ppb of arsenite from contaminated water with these engineered cells, providing a technology useful to comply with the newly approved U.S. Environmental Protection Agency limit of 10 ppb. These results open up the possibility of using cells overexpressing ArsR as an inexpensive, high-affinity ligand for arsenic removal from contaminated drinking and ground water.

scholarly journals Identification and quantification of phytochelatins in roots of rice to long-term exposure: evidence of individual role on arsenic accumulation and translocation

2014 ◽  
Vol 65 (6) ◽  
pp. 1467-1479 ◽  
Author(s):  
Bruno Lemos Batista ◽  
Meher Nigar ◽  
Adrien Mestrot ◽  
Bruno Alves Rocha ◽  
Fernando Barbosa Júnior ◽  
...  
Keyword(s):  
Arsenic Accumulation ◽  
Individual Role ◽  
Identification And Quantification
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