scholarly journals Production of cellulase from trichoderma reesei in fed-batch fermentation from soluble carbon sources

1981 ◽  
Vol 23 (11) ◽  
pp. 2641-2645 ◽  
Author(s):  
A. L. Allen ◽  
R. E. Mortensen
2017 ◽  
Vol 55 (2) ◽  
pp. 195
Author(s):  
Truong Huu Phong ◽  
Dang Minh Khuong ◽  
Duong Van Hop ◽  
Doan Van Thuoc

Yangia sp. ND199 is a halophilic bacterium isolated from mangrove soil sample. This strain was able to produce polyhydroxyalkanoate (PHA) from different carbon sources. Only homopolymer poly(3-hydroxybutyrate) (PHB) was synthesized when fructose was used as carbon source. The bacterium can accumulate high PHB content during exponential phase. Maximum cell dry weight (CDW) of 7.8 g/l and PHB content of 49 wt% were obtained after 27 h of cultivation in batch fermentation. High CDW and PHB content were achieved by using fed-batch fermentation with different fructose feeding strategies. The highest CDW of 78.5 g/l, PHB content of 67.5 wt%, and PHB productivity of 1 g/l/h were obtained by using two-stage fed-batch fermentation, is among the highest reported so far for PHB production by halophilic bacteria.         


Author(s):  
Kaili Zhao ◽  
Yiru Gan ◽  
Zisheng Jason Zhang

Recombinant human interleukin-11(rhIL-11) is still the only therapy drug for thrombocytopenia. Although the biological properties and clinical behaviors of rhIL-11 have been studied extensively, its bioprocess development data have rarely been reported. In this work, fermentation conditions for recombinant E. coli, which expressed interleukin-11 as a fusion protein, were investigated. Batch and fed-batch fermentation experiments were performed in shake flasks and in a BioFlo fermenter with different medium and carbon sources under different operational parameters. Based on the results of the study, M9Ca medium was shown as the better medium and glycerol was identified to be a better carbon source than glucose for the expression of rhIL-11. When glycerol was used as the carbon source in fed-batch fermentation, a dry cell weight of 9.2 g/L and a rhIL-11 expression of 22%, were achieved. The results could be used as references in the optimization of the fermentation process.


2012 ◽  
Vol 18 (5) ◽  
pp. 791
Author(s):  
Xiaoyun DING ◽  
Bin ZHUGE ◽  
Huiying FANG ◽  
Hong ZONG ◽  
Xiaoxiao LIU ◽  
...  

2010 ◽  
Vol 33 (9) ◽  
pp. 1488-1494 ◽  
Author(s):  
W.-H. Huang ◽  
G. S. Shieh ◽  
F.-S. Wang

Sign in / Sign up

Export Citation Format

Share Document